LOCUS       sequence-43218-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Dmitriev PV, Vassetzky YS
  TITLE     A set of vectors for introduction of antibiotic resistance genes by 
            in vitro Cre-mediated recombination.
  JOURNAL   BMC Res Notes. 2008 Dec 23;1:135. doi: 10.1186/1756-0500-1-135.
  PUBMED    19105832
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2008-12-23"; volume: "1:135. doi"; pages: " 
            10.1186/1756-0500-1-135"
COMMENT     Sequence Label: pT-FLAG
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(150..169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(151..169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    443..564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(480..499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     534..553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(708..729)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctggttagtg aaccgtcaga tccgctagca ccatggatta caaagacgat gacgataaac
       61 ctagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
      121 tagggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac aattcactcg
      181 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat ttttaagtgt
      241 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca gtcccaaggc
      301 tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt
      361 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      421 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      541 gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat
      601 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
      661 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
      721 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
      781 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttgg
//
