LOCUS       sequence-432186-        6483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of human 20S CP proteasome subunit beta 
            type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: pACEBac1-PSMB5-PSMB6-PSMB7
FEATURES             Location/Qualifiers
     source          1..6483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(387..478)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    634..768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1721..1812)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1968..2102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3127..3218)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    3295..3328
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(3399..3404,3405..3421,3422..3427)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3616..4149
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4216..4439
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(4574..5162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4654..4673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5352..5517
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    5669..5803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gctccaggtt ggaagaaatg agctgggttg tcatgtctgt gtctgcagct gtcccagcac
       61 cacaacaata aatattagga gatatgaagt gtatttttga acagttcttg tcagcaacaa
      121 ccatcccttc agttgctctt gtatctgctc caagaactat gccatcctta tagaccaccc
      181 cagcgatggt cgtgccagtt ttccggacct ttggaagctt gtatcccctc tttgcaaaat
      241 cggcttccaa gacggcattc ctgcggcagt tatcaaaaga gaagcctcca actggtggag
      301 catacaccga cacagccgcc atgaattccg cgcgcttcgg accgggatcc gcgcccgatg
      361 gtgggacggt atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga
      421 aaacagtaaa atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct
      481 attaatattc cggagtaggt cgatttaaac atcaggcatc attaggttta tttaaatggt
      541 tatgatagtt attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta
      601 gcagccggat cttctaggct caagcagtga tcagatccag acatgataag atacattgat
      661 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      721 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      781 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      841 aacctctaca aatgtggtat ggctgattat gatcctctag tacttctcga caagcttgtc
      901 gagactgcag gctctagatt cgaaagcggc cgcttatcag gcgggtggta aagtggcaac
      961 ggcgaatttg ggtatctggt ctcccaaaag tacttgccgc tctacccctg actctgcaat
     1021 ggctgccagg cggatcactc ctccactgga gccatcccgc tccatggcca aagcgagagc
     1081 attggcagtg aattgcagac actcttcctt ggtcatgcct tcccggtagg tagcatcaac
     1141 atagccatag atgtaggagc tcccggagcc tccaatggca aaggactgcc ttaccatcat
     1201 accccccata ggcactgagt acccctgccc tccttcttga gggtcccagc ctgcgatgat
     1261 gattcccgcc atcaggtctt cccggtatcg gtaacacatc tccttaaaga ggctggctgc
     1321 tgtgtggacc agtggaggct cattcagttc aatgctgtgg aaaccgagct ggtaggtgac
     1381 agcatcagct actgcctggg tatcagcagc tgagcctgag cgacagcaga aaatgcggtc
     1441 gtgaataggt gtcagcttgt cagtcactcg attggcgatg taggacccag tggttgttct
     1501 ggagtccgcc cccagaacca cgcccccgtc aaactgcacg gccatgatag tcgtcccagt
     1561 ggaaacttct cggctttccc agtctggagt gaatgcctcc ggcccccaag ccggtgctgg
     1621 cccggctccc cgagcagcta gtaaggtagc cgccatgaat tccgcgcgct tcggaccggg
     1681 atccgcgccc gatggtggga cggtatgaat aatccggaat atttataggt ttttttatta
     1741 caaaactgtt acgaaaacag taaaatactt atttatttgc gagatggtta tcattttaat
     1801 tatctccatg atctattaat attccggagt aggtcgattt aaacgtgcaa tagtatccag
     1861 tttatttaaa tggttatgat agttattgct cagcggtggc agcagccaac tcagcttcct
     1921 ttcgggcttt gttagcagcc ggatcttcta ggctcaagca gtgatcagat ccagacatga
     1981 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2041 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2101 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2161 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagtacttc
     2221 tcgacaagct tgtcgagact gcaggctcta gattcgaaag cggccgctta tcagggggta
     2281 gagccactat acttctcatg tagatcagcc acattgtcac tggagactcg gatccagcca
     2341 tcctcccgca cgtggtagag gttgactgca cctcctgagt aggcatctct gtaggtggct
     2401 tggtagatgg ctcgacgggc cagatcatag gcctgctcca cttccaggtc ataggaatag
     2461 ccccgatcca tgaccccata tgcatacaca gagccagaac ctacagagaa ggtggcccct
     2521 gaaatccggt tcccttcact gtccacgtag tagaggccag ggcctctctt atcccagcca
     2581 cagatcatgg tgcccatgga cagccccatg cctttgtact gatacaccat gttggcaagc
     2641 agtttggagg cagctgctac agagatgcgt tccttatttc gaagctcata gattcgacat
     2701 tgccgagcca acagccgttc ccagaagctg caatccgctg cgcccccagc catggtgcct
     2761 agcaggtatg ggttgatctc tatcaccttc ttcaccgtct gggaggcaat gtaagcaccc
     2821 gctgtagccc tggagtcagc tgcaactatg actccatggc ggaacttgaa ggccagggtg
     2881 gttgttccat gaagcatttc gattcctggc tcttctggga caccccagcc tggcgcggcc
     2941 aggctcagac catcactgag actccctgga cctagatcca gcagatctgc acgaccccca
     3001 agtccgaaaa acccgcgctg gttcaccggt agcggtctct ccaacacgct cgcaagcgcc
     3061 atgaattccg cgcgcttcgg accgggatcc gcgcccgatg gtgggacggt atgaataatc
     3121 cggaatattt ataggttttt ttattacaaa actgttacga aaacagtaaa atacttattt
     3181 atttgcgaga tggttatcat tttaattatc tccatgatct attaatattc cggagtaggt
     3241 cgcgaatcga tactagtgtt taaactcgct accttaggac cgttatagtt acagataact
     3301 tcgtataatg tatgctatac gaagttatgg cgacttcgct gctgcccaag gttgccgggt
     3361 gacgcacacc gtggaaacgg atgaaggcac gaacccagtt gacataagcc tgttcggttc
     3421 gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     3481 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac
     3541 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     3601 tatggagcag caacgatgtt acgcagcagc aacgatgtta cgcagcaggg cagtcgccct
     3661 aaaacaaagt taggtggctc aagtatgggc atcattcgca catgtaggct cggccctgac
     3721 caagtcaaat ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg agacgtagcc
     3781 acctactccc aacatcagcc ggactccgat tacctcggga acttgctccg tagtaagaca
     3841 ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt
     3901 ctgcccaagt ttgagcagcc gcgtagtgag atctatatct atgatctcgc agtctccggc
     3961 gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac
     4021 gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct
     4081 ctctatacaa agttgggcat acgggaagaa gtgatgcact ttgatatcga cccaagtacc
     4141 gccacctaac aattcgttca agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa
     4201 ttgtcacaac gccgcgaata tagtctttac atgcccttgg ccacgcccct ctttaatacg
     4261 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta
     4321 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga
     4381 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac
     4441 ccgcttacgc agggcatctg cgcatccaca ggaagagcga cccaagtcaa tcaggggata
     4501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttgaccca
     5341 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     5401 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     5461 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcca
     5521 attagatggg tataccctag gaaactaagc tatgtgaacc gtttatttaa atggttatga
     5581 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     5641 cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca ttgatgagtt
     5701 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5761 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5821 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     5881 ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc ttgtcgagac
     5941 tgcaggctct agattcgaaa gcggccgctt atcaggaagt gtccattgtt tggactgttt
     6001 cttccagcac ctcaatctcc agaggagtga ttttctcagt gaggactgca gtagtccctt
     6061 tctcacacct gtaccggcca agcctggtcc ccttcttgtt gggcactgtg tatgggcgga
     6121 gaaaatccag cttgttcttg ctgatgacgc agaggtcaat gttgcttccg gagcccaggt
     6181 cgttgaagat gccagctgcg atggcttcgc tcaccagatt cttcgcttcc tcctcctcca
     6241 tgtctggcct aaacttatct tcaaatacag ccattgctgc caaggagcca gaacccatgg
     6301 tgacataagg caacttatca gttgatccat gaggatagat gctgtagagg tgaggtccag
     6361 taacatctac tccccctaaa actagggctg caccaatgta accttgatac ctgaaaagca
     6421 tctgcttcag catccgattg gctgtcacaa ctctgggaag acggccagtg gagagggagt
     6481 gga
//
