LOCUS       sequence-432188-       10627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of EcSTH which 
            is a soluble transhydrogenase from E. coli that can be used to 
            increase NADH/NAD+ ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-EcSTH-FLAG
FEATURES             Location/Qualifiers
     source          1..10627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             34..633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(34..53)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     530..550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    647..1235
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(700..720)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1118..1129)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1442..2075
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2203..2219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2203..2219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2227..2243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2251..2257,2258..2275,2276..2281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2296..2317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3364..4155,4156..4224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3987..4006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4225..4329)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4377..4511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             4679..5312
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5359..5484
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5981..6214
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6399..6443
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6706..6821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    6865..6999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7195..7218)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8653..9020)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8778..8796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8814..8832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8850..8868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8886..8904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8922..8940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8958..8976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8994..9012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9037..9547
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9466..9484
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9566..10312
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        join(10323..10627,1..25)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10503..10627,1..11)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttttggaggc ctaggctttt gcaaaacgcg accatgaccg agtacaagcc cacggtgcgc
       61 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
      121 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg
      181 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
      241 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
      301 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
      361 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
      421 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
      481 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
      541 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
      601 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtc tggaacaatc aacctctgga
      661 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
      721 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
      781 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
      841 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
      901 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
      961 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     1021 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     1081 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     1141 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     1201 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     1261 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     1321 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     1381 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     1441 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     1501 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     1561 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     1621 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     1681 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     1741 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     1801 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     1861 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     1921 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1981 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2041 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     2101 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     2161 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     2221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2281 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2341 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4321 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4501 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     4561 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     4621 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     4681 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     4741 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     4801 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     4861 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     4921 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     4981 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     5041 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     5101 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     5161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5281 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     5341 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     5401 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     5461 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     5521 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     5581 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     5641 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     5701 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     5761 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     5821 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     5881 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     5941 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6001 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6061 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6121 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6181 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6241 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     6301 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     6361 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     6421 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     6481 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     6541 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     6601 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     6661 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     6721 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     6781 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     6841 agcagagatc cagtttatcg acttaacttg tttattgcag cttataatgg ttacaaataa
     6901 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
     6961 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
     7021 cctgtcttag gttggagtga tacattttta tcacttttac ccgtctttgg attaggcagt
     7081 agctctgacg gccctcctgt cttaggttag tgaaaaatgt cactctctta cccgtcattg
     7141 gctgtccagc ttagctcgca ggggaggtgg tctggatccg ccggcaccgg tttacttgtc
     7201 atcgtcatcc ttgtaatcca tagatccacc agatccacca gatccaccga acagtctgtt
     7261 taaaccattg agggcggcca cacgataggc ttcggccatg gtggggtagt tgaaggtggt
     7321 gttcacgaag tactcgatgg tgttgccgcc gcccttctgt tccatgatag cttggccaat
     7381 gtggatgatc tcagcagctc tctctccgaa acagtggatg cccagaatct ccttggtctc
     7441 acgatgaaag aggatcttga gggtgcccac gttcatgccc acgatctggg cacgagctaa
     7501 atgtttgaac tgggctcttc ccacctcgta agggactttc atggcggtca gctgctgctc
     7561 tgtcttgccc acgctgctga tctcggggat ggtgtagatg ccggtgggaa tgtcctcgat
     7621 taaatgggcg gtggcctctc ctttgactaa agcttgagca gcgatacgac cttggtcgta
     7681 agcggcggaa gctaaagaag ggtagccgat cacatctccc acggcgtaca catggggctg
     7741 ggctgtctgg tacatgctgt tcactttcag ctggccacga gagtcggtct ccagtccgat
     7801 gttctggaga gctaaagagt cggtgttgcc ggtacgaccg ttggcataga gtaaacagtc
     7861 ggccttcagc ttcttgccgg actttaaatg catgatcacg ccgtcgtcgc agccctcaat
     7921 cttctcgtac tcctcgttgt gacgaatcac gactccggag ttccagaagt ggtagcttaa
     7981 agaatcgctc atttcttgat ccagaaaggc gagcagccta tcacgagtgt tgattaaatc
     8041 caccttcacg tccattccac gaaagatgct agcgtactcg cagccgatca cgccggcgcc
     8101 gtagatcagc acgtgtctgg gctcgtgatg catgcttaaa atgctgtcgc tgtcatagat
     8161 acgagggtgg gtgaagtcca cgtcggtggg gtggtagggt ctgctgccac aagcaatcac
     8221 gaacttctcg gctgttaaag tctccacgga gccatcgggg cagtcgaggg ctaaagtgtg
     8281 ttcgtccacg aatctagcgt taccttgtaa aatctcgcag tggttcctct cgtagaaacc
     8341 ttgcctcatt ctggtctgtt ggttgatgac gttatcggcg tggtttaaaa tgtcggcgaa
     8401 gctgcttctt aataaacgag agtggtcgga gtataaaggg ttctggttga actcaatgat
     8461 acgagacacg gcgtgcctta aagctttgct agggattgtt ccccagtggg tacagccgcc
     8521 gcccacattc tggtacctct caatgacggc caccctagca ccttgcttga ctaaacccat
     8581 ggcagcgcct tctccaccgg gtccgctgcc gatcacgatg gcgtcgtaat cgtagctgtg
     8641 gggcatgaat tctttacgag ggtaggaagt ggtacggaaa gttggtataa gacaaaagtg
     8701 ttgtggaatt gaagtttact caaaaaatca gcactctttt ataggcgccc tggtttacat
     8761 aagcaaagct tatacgttct ctatcactga tagggagtaa actggatata cgttctctat
     8821 cactgatagg gagtaaactg tagatacgtt ctctatcact gatagggagt aaactggtca
     8881 tacgttctct atcactgata gggagtaaac tccttatacg ttctctatca ctgataggga
     8941 gtaaagtctg catacgttct ctatcactga tagggagtaa actcttcata cgttctctat
     9001 cactgatagg gagtaaactc gaggtgataa ttccacgggg ttggggttgc gccttttcca
     9061 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     9121 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     9181 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     9241 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     9301 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     9361 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     9421 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     9481 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     9541 tccccagggg gatcatcgaa ttaccatgtc tagactggac aagagcaaag tcataaactc
     9601 tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     9661 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     9721 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg
     9781 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     9841 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     9901 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     9961 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
    10021 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca
    10081 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
    10141 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
    10201 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
    10261 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aaacgcgcga
    10321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10561 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10621 gaggctt
//
