LOCUS       sequence-432197-       11101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tTa-inducible miRFP703, with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11101)
  TITLE     Engineering Programmable Material-To-Cell Pathways Via Synthetic 
            Notch Receptors To Spatially Control Cellular Phenotypes In 
            Multi-Cellular Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHR-TRE-miRFP703_PGK-Puro
FEATURES             Location/Qualifiers
     source          1..11101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      911..1499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1400..1419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1653..1670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1740..1760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1745..2074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1925..2060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3208..3273
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3403..3423
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3848..3982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3885..3904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3939..3958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4150..4783
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4830..4955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5452..5685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5870..5914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6199..6316
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6378..6727
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    6386..6404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6422..6440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6458..6476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6494..6512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6530..6548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6566..6584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6602..6620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6666..6690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6737..6746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6743..7690
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    7788..8376
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7841..7861)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8259..8270)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8379..8395)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8380..8396)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        8427..8926
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8462..8485)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8859..8878
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8936..9535
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8936..8955)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9432..9452
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9812..10045
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10070..10088)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10071..10088)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10378..10397)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10611..10630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10730..10752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10790..10808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10876..10980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10981..11049,11050..11101,1..740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
       61 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     1741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1801 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1981 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2041 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     2101 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     2161 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     2221 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     2281 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     2341 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     2401 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     2461 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     2521 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     2581 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     2641 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     2701 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     2761 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     2821 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     2881 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     2941 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     3001 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     3061 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     3121 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     3181 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     3241 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     3301 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     3361 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     3421 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     3481 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     3541 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     3601 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     3661 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     3721 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     3781 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     3841 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3961 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     4021 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     4081 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     4141 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     4201 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     4261 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     4321 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     4381 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     4441 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     4501 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     4561 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     4621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     4801 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     4861 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     4921 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     4981 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     5041 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     5101 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     5161 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     5221 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     5281 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     5341 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     5401 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     5461 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     5521 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     5581 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     5641 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     5701 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     5761 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     5821 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     5881 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     5941 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     6001 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     6061 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     6121 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     6181 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     6241 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     6301 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt atcgatgagg
     6361 ccctttcgtc ttcactcgag tttactccct atcagtgata gagaacgtat gaagagttta
     6421 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     6481 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     6541 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     6601 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa
     6661 agcagagctc gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct
     6721 tatacttacg cgtgccgcca ccatggtagc aggtcatgcc tctggcagcc ccgcattcgg
     6781 gaccgcctct cattcgaatt gcgaacatga agagatccac ctcgccggct cgatccagcc
     6841 gcatggcgcg cttctggtcg tcagcgaaca tgatcatcgc gtcatccagg ccagcgccaa
     6901 cgccgcggaa tttctgaatc tcggaagcgt actcggcgtt ccgctcgccg agatcgacgg
     6961 cgatctgttg atcaagatcc tgccgcatct cgatcccacc gccgaaggca tgccggtcgc
     7021 ggtgcgctgc cggatcggca atccctctac ggagtactgc ggtctgatgc atcggcctcc
     7081 ggaaggcggg ctgatcatcg aactcgaacg tgccggcccg tcgatcgatc tgtcaggcac
     7141 gctggcgccg gcgctggagc ggatccgcac ggcgggttca ctgcgcgcgc tgtgcgatga
     7201 caccgtgctg ctgtttcagc agtgcaccgg ctacgaccgg gtgatggtgt atcgtttcga
     7261 tgagcaaggc cacggcctgg tattctccga gtgccatgtg cctgggctcg aatcctattt
     7321 cggcaaccgc tatccgtcgt cgctggtccc gcagatggcg cggcagctgt acgtgcggca
     7381 gcgcgtccgc gtgctggtcg acgtcaccta tcagccggtg ccgctggagc cgcggctgtc
     7441 gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc ctgcgctcga tgtcgccgat
     7501 ccatctgcag ttcctgaagg acatgggcgt gcgcgccacc ctggcggtgt cgctggtggt
     7561 cggcggcaag ctgtggggcc tggttgtctg tcaccattat ctgccgcgct tcatccgttt
     7621 cgagctgcgg gcgatctgca aacggctcgc cgaaaggatc gcgacgcgga tcaccgcgct
     7681 tgagagctaa gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact
     7741 ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg
     7801 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7861 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7921 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7981 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8041 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8101 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8161 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8221 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8281 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8341 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga
     8401 attaattcga gctcggtacc tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     8461 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     8521 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     8581 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     8641 caggacgtga caaatggaag tagcacgact cactagtctc gtgcagatgg acagcaccgc
     8701 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     8761 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     8821 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     8881 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgtccg ccaccatgac
     8941 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9001 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9061 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9121 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9181 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9241 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9301 gtggttcctg gccaccgtcg gcgtgtcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9361 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9421 atccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9481 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgactcgc
     9541 tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact
     9601 gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt
     9661 cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga atgtgggagg
     9721 tcagtgcatt taaaacataa agaaacttta agaccaatga cttacaaggc agctgtagat
     9781 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9841 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     9901 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9961 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10021 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
    10081 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
    10141 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
    10201 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
    10261 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
    10321 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
    10381 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
    10441 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
    10501 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
    10561 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
    10621 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
    10681 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    10741 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
    10801 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
    10861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    10921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    10981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    11041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    11101 c
//
