LOCUS       sequence-432199-       10696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of mitoEcSTH 
            which is a soluble transhydrogenase from E. coli that can be used to
            increase NADH/NAD+ ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-mitoEcSTH-FLAG
FEATURES             Location/Qualifiers
     source          1..10696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(371..415)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    600..833
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1330..1455
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1502..2135
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2303..2437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2485..2589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2590..2658,2659..3450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2808..2827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3621..4209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4110..4129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4363..4380
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4497..4518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4533..4538,4539..4556,4557..4563)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4571..4587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4576..4598
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4595..4611
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4595..4611
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4739..5372
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5579..6167
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5685..5696
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6094..6114
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6181..6780)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6264..6284)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6761..6780
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6789..7118)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6803..6938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7129..7875)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7894..8404)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7957..7975)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8421..8788
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8429..8447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8465..8483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8501..8519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8537..8555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8573..8591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8609..8627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8645..8663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             10292..10315
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    10511..10645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(10689..10696,1..108)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
       61 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaagg cgataccgtc
      121 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
      181 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
      241 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
      301 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
      361 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
      421 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
      481 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
      541 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
      601 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
      661 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
      721 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
      781 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
      841 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
      901 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
      961 ctcctccagg tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt
     1021 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
     1081 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
     1141 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
     1201 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     1261 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     1321 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     1381 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     1441 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     1501 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     1561 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     1621 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     1681 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt
     1741 cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg
     1801 atatcagcag ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag
     1861 gcggctgtca aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct
     1921 cacagggtgt aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc
     1981 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg
     2041 tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat
     2101 atcttgtctt ctttgggagt gaattagccc ttccaactac taagtttgta gtacatattt
     2161 aacaaataca atttctttaa aatgaaaata attcagagga atcacaggtt tagagtaaat
     2221 gaaaccacag gtaattggca gtggtaatag ggtatggggt gggaagtttg ggatgatttt
     2281 ggttagcttg agttatccag ttgatccaga catgataaga tacattgatg agtttggaca
     2341 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2401 tttatttgta accattataa gctgcaataa acaagttgat ctcccgatcc gtcgacgtca
     2461 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2821 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4321 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4381 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4441 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4501 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4561 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     4621 gccaagctct agctagaggt cgacggtaaa cgctagcaat gtcaaggcct ctcactctct
     4681 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg
     4741 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     4801 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     4861 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     4921 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     4981 gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc aagctcgata
     5041 tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg
     5101 gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat gcaggctcac
     5161 agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat ctggctcaac
     5221 tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg gccctggtgt
     5281 gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat caaggatatc
     5341 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccctc ttttctttta aaaagtggct
     5401 aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctgaggtg tgactggaaa
     5461 acccacctcc tcctcctctt gtgcttctag ccaggcacaa tcagcattgg tagctgctgt
     5521 attgctactt gtgattgctc catgtttttc taggtctcga ctgcagaatt aattccaggc
     5581 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     5641 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     5701 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga
     5761 aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     5821 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     5881 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     5941 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     6001 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     6061 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     6121 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtt ccagacgcgt
     6181 tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
     6241 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc
     6301 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct
     6361 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg
     6421 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga
     6481 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct
     6541 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat
     6601 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg
     6661 gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg
     6721 tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat
     6781 ggtcgcgttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     6841 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     6901 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     6961 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     7021 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     7081 ggggagcctg gggactttcc acaccctaac tgacacacat tcgcgcgttt acccggggag
     7141 catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt caaagtcgtc
     7201 aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc
     7261 gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc cgaaaaggaa
     7321 ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat
     7381 agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct gttcctccaa
     7441 tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt tctccaggga
     7501 gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact gtttctctgt
     7561 tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag cacagcggta
     7621 tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg
     7681 ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc gcttgttctt
     7741 cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt
     7801 caggccttcg ataccgactc cattgagtaa ttccagagca gagtttatga ctttgctctt
     7861 gtccagtcta gacatggtaa ttcgatgatc cccctgggga gagaggtcgg tgattcggtc
     7921 aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc
     7981 gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg
     8041 gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg
     8101 gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc
     8161 ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg
     8221 agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg
     8281 aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg
     8341 cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa
     8401 ccccgtggaa ttatcacctc gagtttactc cctatcagtg atagagaacg tatgaagagt
     8461 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     8521 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     8581 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     8641 ttactcccta tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat
     8701 aaaagagtgc tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac
     8761 tttccgtacc acttcctacc ctcgtaaaga attcatgctg gccaccaggg tctttagcct
     8821 ggtgggcaaa agagccatct ccacatccgt ctgcgtgaga gcccatcccc acagctacga
     8881 ttacgacgcc atcgtgatcg gcagcggacc cggtggagaa ggcgctgcca tgggtttagt
     8941 caagcaaggt gctagggtgg ccgtcattga gaggtaccag aatgtgggcg gcggctgtac
     9001 ccactgggga acaatcccta gcaaagcttt aaggcacgcc gtgtctcgta tcattgagtt
     9061 caaccagaac cctttatact ccgaccactc tcgtttatta agaagcagct tcgccgacat
     9121 tttaaaccac gccgataacg tcatcaacca acagaccaga atgaggcaag gtttctacga
     9181 gaggaaccac tgcgagattt tacaaggtaa cgctagattc gtggacgaac acactttagc
     9241 cctcgactgc cccgatggct ccgtggagac tttaacagcc gagaagttcg tgattgcttg
     9301 tggcagcaga ccctaccacc ccaccgacgt ggacttcacc caccctcgta tctatgacag
     9361 cgacagcatt ttaagcatgc atcacgagcc cagacacgtg ctgatctacg gcgccggcgt
     9421 gatcggctgc gagtacgcta gcatctttcg tggaatggac gtgaaggtgg atttaatcaa
     9481 cactcgtgat aggctgctcg cctttctgga tcaagaaatg agcgattctt taagctacca
     9541 cttctggaac tccggagtcg tgattcgtca caacgaggag tacgagaaga ttgagggctg
     9601 cgacgacggc gtgatcatgc atttaaagtc cggcaagaag ctgaaggccg actgtttact
     9661 ctatgccaac ggtcgtaccg gcaacaccga ctctttagct ctccagaaca tcggactgga
     9721 gaccgactct cgtggccagc tgaaagtgaa cagcatgtac cagacagccc agccccatgt
     9781 gtacgccgtg ggagatgtga tcggctaccc ttctttagct tccgccgctt acgaccaagg
     9841 tcgtatcgct gctcaagctt tagtcaaagg agaggccacc gcccatttaa tcgaggacat
     9901 tcccaccggc atctacacca tccccgagat cagcagcgtg ggcaagacag agcagcagct
     9961 gaccgccatg aaagtccctt acgaggtggg aagagcccag ttcaaacatt tagctcgtgc
    10021 ccagatcgtg ggcatgaacg tgggcaccct caagatcctc tttcatcgtg agaccaagga
    10081 gattctgggc atccactgtt tcggagagag agctgctgag atcatccaca ttggccaagc
    10141 tatcatggaa cagaagggcg gcggcaacac catcgagtac ttcgtgaaca ccaccttcaa
    10201 ctaccccacc atggccgaag cctatcgtgt ggccgccctc aatggtttaa acagactgtt
    10261 cggtggatct ggtggatctg gtggatctat ggattacaag gatgacgatg acaagtaaac
    10321 cggtgccggc ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
    10381 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
    10441 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
    10501 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
    10561 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
    10621 cattataagc tgcaataaac aagttaagtc gataaactgg atctctgctg tccctgtaat
    10681 aaacccgaaa attttg
//
