LOCUS       sequence-432200-       11687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..11687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..667
                     /label=chicken beta-actin promoter
     intron          668..1684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1608..1630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1692..1711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2872..3460
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2925..2945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3343..3354)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3551..3570)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3616..3671
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3670..3689)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4032..4048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4032..4048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4045..4067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4056..4072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4080..4086,4087..4104,4105..4110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4125..4146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4235..4268
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4729..6654
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6822..6855
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7042..8831
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        9301..9614
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     9533..9553
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(9765..9782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9936..10524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10016..10035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10695..11486,11487..11555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11318..11337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11556..11660)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgc
     1741 ccttcaccat ggcgggacac ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg
     1801 gaggtgatgg gccagggggg ccggagccgg gctgggttga tcctcggacc tggctaagct
     1861 tccaaggccc tcctggaggg ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt
     1921 gggggattcc cccatgcccc ccgccgtatg agttctgtgg ggggatggcg tactgtgggc
     1981 cccaggttgg agtggggcta gtgccccaag gcggcttgga gacctctcag cctgagggcg
     2041 aagcaggagt cggggtggag agcaactccg atggggcctc cccggagccc tgcaccgtca
     2101 cccctggtgc cgtgaagctg gagaaggaga agctggagca aaacccggag gagtcccagg
     2161 acatcaaagc tctgcagaaa gaactcgagc aatttgccaa gctcctgaag cagaagagga
     2221 tcaccctggg atatacacag gccgatgtgg ggctcaccct gggggttcta tttgggaagg
     2281 tattcagcca aacgaccatc tgccgctttg aggctctgca gcttagcttc aagaacatgt
     2341 gtaagctgcg gcccttgctg cagaagtggg tggaggaagc tgacaacaat gaaaatcttc
     2401 aggagatatg caaagcagaa accctcgtgc aggcccgaaa gagaaagcga accagtatcg
     2461 agaaccgagt gagaggcaac ctggagaatt tgttcctgca gtgcccgaaa cccacactgc
     2521 agcagatcag ccacatcgcc cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt
     2581 tctgtaaccg gcgccagaag ggcaagcgat caagcagcga ctatgcacaa cgagaggatt
     2641 ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc ctttcctctg gccccagggc
     2701 cccattttgg taccccaggc tatgggagcc ctcacttcac tgcactgtac tcctcggtcc
     2761 ctttccctga gggggaagcc tttccccctg tctctgtcac cactctgggc tctcccatgc
     2821 attcaaactg aggtaagggc gaattcaagc ttcggggact agtcatatga taatcaacct
     2881 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2941 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3001 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3061 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3121 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3181 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3241 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3301 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3361 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3421 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcggtacaa ttcactcctc
     3481 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     3541 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     3601 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3661 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     3721 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     3781 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     3841 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     3901 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     3961 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     4021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     4201 atctcaattc cgatcatatt caataaccct taatataact tcgtataatg tatgctatac
     4261 gaagttatta ggtctgaaga ggagtttacg tccagccaag cttaggatca attctcatgt
     4321 ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
     4381 ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
     4441 catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
     4501 acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
     4561 acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
     4621 aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
     4681 tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
     4741 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
     4801 ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
     4861 agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
     4921 agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
     4981 acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
     5041 gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggaggggca
     5101 ggagcaggag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     5161 ggaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga
     5221 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
     5281 ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
     5341 ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggaggggca
     5401 ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg
     5461 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     5521 ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca
     5581 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     5641 gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca
     5701 ggaggtggag gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc
     5761 cggggtcgag gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga
     5821 agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc
     5881 agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca
     5941 tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat
     6001 ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa
     6061 ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg
     6121 tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt
     6181 ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc
     6241 gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact
     6301 gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc
     6361 cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc
     6421 tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg
     6481 acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta
     6541 gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga
     6601 gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac
     6661 ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa
     6721 atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat
     6781 tttcgtcctc ccaacatggg gcaattgccg gaacccttaa tataacttcg tataatgtat
     6841 gctatacgaa gttattaggt ccctcgaaga ggttcactag cggatctcaa ttgggcatac
     6901 ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga aaacattagc
     6961 gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc cttaaattca
     7021 cctaagaatg ggagcaacca gcaggaaaag gacaagcagc gaaaattcac gcccccttgg
     7081 gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat catatgctga
     7141 ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag catatactac
     7201 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     7261 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     7321 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctac
     7381 ccagatatag attaggatag catatgctat ccagatattt gggtagtata tgctacccag
     7441 atataaatta ggatagcata tactacccta atctctatta ggatagcata tgctacccgg
     7501 atacagatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
     7561 atatagatta ggatagccta tgctacccag atataaatta ggatagcata tactacccag
     7621 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
     7681 atatagatta ggatagcata tgctatccag atatttgggt agtatatgct acccatggca
     7741 acattagccc accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt
     7801 ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc
     7861 ttggcccgcc cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc
     7921 aagtggtttg accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt
     7981 ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt
     8041 tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt
     8101 taattttcgg gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct
     8161 ttccccttgt tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg
     8221 acacatctta ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata
     8281 aattcgtgtg agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt
     8341 gttaaagata ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg
     8401 agggttatat tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc
     8461 tgggggcgtc acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac
     8521 tcagcagtta ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag
     8581 agttcactgc ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac
     8641 cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat
     8701 cgctgttcct taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt
     8761 aacatatgct attgaattag ggttagtctg gatagtatat actactaccc gggaagcata
     8821 tgctacccgt ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga
     8881 gggtccgctt atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta
     8941 gtcttcctgg gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa
     9001 gagttacaca taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga
     9061 tgaaagccac tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc
     9121 agagaacccc tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc
     9181 aagttgtacc aaccaactga agggattaca tgcactgccc cgcgaagaag gggcagagat
     9241 gtcgtagtca ggtttagttc gtccggggcg gggatcgatc ctctagagtc gacgctagcg
     9301 gatccgacgc cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag
     9361 ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc
     9421 ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga
     9481 taggcttgga tttctataag agatacaaat actaaattat tattttaaaa aacagcacaa
     9541 aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg
     9601 agaaaagcct tgtttgggcc gactccagtg gtaatctact tcaagagagt agattaccac
     9661 tggagtcttt tttggaattc ctgcagcccg ggggatccgc tgcattaatg aatcggccaa
     9721 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10801 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgg
//
