LOCUS       sequence-432203-        9858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-color fluorescent reporter for CD63-positive exosome secretion 
            and uptake.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, 
            Pelletier R, Zijlstra A, Ponik SM, Weaver AM
  TITLE     A live cell reporter of exosome secretion and uptake reveals 
            pathfinding behavior of migrating cells.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2092. doi: 10.1038/s41467-020-15747-2.
  PUBMED    32350252
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2092. doi"; pages: " 
            10.1038/s41467-020-15747-2"
COMMENT     Sequence Label: pLenti-pHluorin_M153R-CD63-mScarlet
FEATURES             Location/Qualifiers
     source          1..9858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    613..637
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             787..1497
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIRADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             787..1497
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIRADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(814..842)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1437..1457
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2089..2784
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2222..2240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2490..2509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2810..2834)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2865..3453
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2918..2938)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3336..3347)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3504..3524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3509..3838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3689..3824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3886..3933
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3952..4350
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4437..4670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4742..4863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4779..4798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4833..4852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4903..5038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5058..5077)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5059..5077)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5087..5104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5087..5103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5096..5118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5245..5700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5332..5351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5542..5563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5726..5830
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5831..5899,5900..6691)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6049..6068)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6862..7450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7351..7370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7604..7621
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7738..7759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7774..7779,7780..7797,7798..7804)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7812..7828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7817..7839
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7836..7852
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7836..7852
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7871..7891
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7873..7891
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7919..8145
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8146..8326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8373..8498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8991..9224
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9409..9453
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9720..9836
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg
      661 gcggtggaag gaggaatgaa atgtgtgaag ttcttgctct acgtcctcct gctggccttt
      721 tgcgcctgtg cagtgggact gattgccgtg ggtgtcgggg cacagcttgt cctgagtcag
      781 accataagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      841 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      901 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      961 cttgtcacta ctctcactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
     1021 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1081 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1141 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1201 aaattggaat acaactataa cgatcaccag gtgtacatcc gggcagacaa acaaaagaat
     1261 ggaatcaaag ctaacttcaa aattagacac aacattgaag atggaggcgt tcaactagca
     1321 gaccattatc aacaaaatac tccaattggc gatgggcccg tccttttacc agacaaccat
     1381 tacctgttta caacttctac tctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1441 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaatc
     1501 cagggggcta cccctggctc tctgttgcca gtggtcatca tcgcagtggg tgtcttcctc
     1561 ttcctggtgg cttttgtggg ctgctgcggg gcctgcaagg agaactattg tcttatgatc
     1621 acgtttgcca tctttctgtc tcttatcatg ttggtggagg tggccgcagc cattgctggc
     1681 tatgtgttta gagataaggt gatgtcagag tttaataaca acttccggca gcagatggag
     1741 aattacccga aaaataacca cactgcttcg atcctggaca ggatgcaggc agattttaag
     1801 tgctgtgggg ctgctaacta cacagattgg gagaaaatcc cttccatgtc gaagaaccga
     1861 gtccccgact cctgctgcat taatgttact gtgggctgtg ggattaattt caacgagaag
     1921 gcgatccata aggagggctg tgtggagaag attgggggct ggctgaggaa aaatgtgctg
     1981 gtggtagctg cagcagccct tggaattgct tttgtcgagg ttttgggaat tgtctttgcc
     2041 tgctgcctcg tgaagagtat cagaagtggc tacgaggtga tggaattcat ggtgagcaag
     2101 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     2161 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2221 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     2281 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
     2341 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     2401 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     2461 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     2521 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
     2581 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     2641 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
     2701 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     2761 ggcggcatgg acgagctgta caagggatcc tagaagggtg ggcgcgccga cccagctttc
     2821 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt
     2881 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2941 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3001 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3061 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3121 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3181 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3241 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3301 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3361 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3421 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggttagt
     3481 aatgagtttg gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     3781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3841 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     3901 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     3961 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     4021 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     4081 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     4141 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     4201 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     4261 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     4321 ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcggtac ctttaagacc
     4381 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4441 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4501 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4561 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4621 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4681 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4741 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4861 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5041 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5101 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5161 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5221 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5281 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5341 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5401 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5461 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5521 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5581 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5641 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5701 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5821 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5881 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5941 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6001 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6061 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6121 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6181 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6241 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6301 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6361 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6421 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6481 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6541 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6601 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6661 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6721 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6781 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7021 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7081 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7141 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7201 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7261 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7321 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7441 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7501 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7561 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7621 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7681 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7741 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7801 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7861 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7921 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     7981 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8041 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     8101 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     8161 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8221 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8281 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     8341 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8401 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     8461 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     8521 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     8581 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     8641 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     8701 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     8761 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8821 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8881 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8941 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9001 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9061 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9121 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9181 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9241 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     9301 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9361 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9421 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9481 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     9541 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     9601 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     9661 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     9721 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9781 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     9841 ataagcttgg gagttccg
//
