LOCUS       sequence-43222-e        6316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-Puro-MEK-DD
FEATURES             Location/Qualifiers
     source          1..6316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             281..750
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(879..896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1050..1638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1130..1149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1798..2589,2590..2658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2421..2440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2659..2763)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2794..3263
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3328..3527
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3728..4144
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4074..4096
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4112..4128
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5433..5453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5438..5767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5618..5753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5777..6316,1..60)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5777..5796)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6273..6293
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
       61 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata
      121 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
      181 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
      241 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
      301 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
      361 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
      421 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
      481 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
      541 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
      601 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
      661 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
      721 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
      781 tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg
      841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1741 atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2761 gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac aggatatctg
     2821 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     2881 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     2941 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     3001 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     3061 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     3121 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     3181 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     3241 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     3301 gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt gtctgtccga
     3361 ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
     3421 ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg
     3481 gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat
     3541 gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt
     3601 cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct
     3661 gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga
     3721 aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
     3781 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     3841 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     3901 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     3961 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     4021 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     4081 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     4141 cgccggccgg atccatgccc aagaagaagc cgacgcccat ccagctgaac ccggcccccg
     4201 atggctcggc ggttaacggg accagctcgg ccgagaccaa cctggaggcc ttgcagaaga
     4261 agctggagga gctggagctt gacgagcagc agcggaagcg gctcgaggcc tttctgacgc
     4321 agaagcagaa ggtgggggaa ctgaaggatg atgactttga gaagatcagc gaactgggag
     4381 ctggcaacgg tggagtggtc ttcaaggtct cccacaagcc atctggcctg gttatggcta
     4441 gaaagctgat ccacctggag atcaaacccg caatccggaa ccagatcatc cgggagctgc
     4501 aggtactgca cgagtgcaac tccccgtaca tcgtgggctt ctacggggcc ttctacagcg
     4561 acggcgagat cagcatctgc atggagcaca tggatggtgg gtccttggat caagttctga
     4621 agaaagctgg aagaattcct gagcaaattt taggaaaagt tagcattgct gtgataaaag
     4681 gcctgaccta tcttcgggag aagcacaaga ttatgcacag agatgtcaag ccatccaaca
     4741 ttctagtgaa ctcacgtggg gagatcaaac tctgtgattt tggggtcagc gggcagctaa
     4801 ttgacgatat ggccaacgat ttcgtgggca cgagatccta catgtcgcct gagagactcc
     4861 aggggactca ctactctgtg cagtcggaca tctggagcat ggggctctct ctggtggaga
     4921 tggcagttgg gagatacccc attcctcctc ctgatgccaa ggagctggag ctactgtttg
     4981 gatgccatgt ggaaggagac gcagccgaaa caccacccag gccaaggacc cctgggaggc
     5041 ctctcagctc atatggaatg gacagccgac ctcccatggc aatttttgag ttgttggatt
     5101 acattgtcaa tgagcctcct ccaaaactgc ccagtggagt attcagtctg gagtttcagg
     5161 attttgtgaa taaatgctta ataaagaacc ctgcagagag agcagatctg aagcagctca
     5221 tggtacatgc tttcatcaaa agatctgacg ccgaggaggt agacttcgca ggctggctct
     5281 gctccaccat tgggcttaac cagcccagca caccaaccca cgctgccagc atctaggcgg
     5341 ccgctcgagt ctagagggcc cgtttgtacc accacactgg gatcccagtg tggtggtacg
     5401 taggaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa
     5461 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5521 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5581 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5641 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5701 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5761 ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5821 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5881 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5941 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6001 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6061 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6121 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6181 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6241 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6301 cttcaccgtc accgcc
//
