LOCUS       sequence-432229-        9819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector with lacIq-Ptac expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
  TITLE     A series of vectors for inducible gene expression in 
            multidrug-resistant Acinetobacter baumannii.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 
            10.1128/aem.00474-24. Epub 2024 Aug 20.
  PUBMED    39162403
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-09-18"; volume: "90(9):e0047424. 
            doi"; pages: " 10.1128/aem.00474-24. Epub 2024 Aug 20"
COMMENT     Sequence Label: pMApra-Ptac
FEATURES             Location/Qualifiers
     source          1..9819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..840
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             827..1678
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1955..3037)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2999..3018
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3038..3115)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(3345..3350,3351..3366,3367..3373)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3381..3397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3386..3408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    3414..3470
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3503..3520)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3673..3759
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3851..3878
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3897..3988
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4431..4533
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4600..4619)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4946..5746
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      6276..6670
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7012..7099
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8338..9309
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2041 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     2101 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     2161 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     2221 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     2281 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     2341 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     2401 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     2461 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     2521 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     2581 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     2641 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2701 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2761 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2821 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2881 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2941 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     3001 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     3061 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
     3121 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
     3181 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     3241 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
     3301 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     3361 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
     3421 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg
     3481 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     3541 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     3601 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     3661 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3721 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3781 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     3841 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4141 cggtaagatc cttgagagtt ttcgccccga agaacgtgat gtccggcggt gcttttgccg
     4201 ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact
     4261 ggcctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
     4321 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     4381 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     4441 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     4501 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     4561 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     4621 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttatc aaggcccgat
     4681 ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga
     4741 cccgcagttg caaaccctca ctgatccggc tcacggtaac tgatgccgta tttgcagtac
     4801 cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg
     4861 tgcagctcca tcagcaaaag gggatgataa gtttatcacc acctactatt tgcaacagtg
     4921 ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata
     4981 cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg
     5041 tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg
     5101 actgatcgag gccctgcgtg ctgcgctggg tccgggaggg acgctcgtca tgccctcgtg
     5161 gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg
     5221 agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc
     5281 ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc
     5341 tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct
     5401 cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc
     5461 ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac gcgtcgatta
     5521 tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa
     5581 gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc gctcccgcga
     5641 cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc
     5701 agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag ctcatgaaaa
     5761 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     5821 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     5881 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     5941 gtaatttttt taaggcagtt attgtcttca aattcccgtt gcacatagcc cggcaattcc
     6001 tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag
     6061 agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc
     6121 gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc
     6181 tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg
     6241 ccagccagtg cccgtcactc ccgtactaac tgtcacgaac ccctgcaata actgtcacgc
     6301 ccccctgcaa taactgtcac gaacccctgc aataactgtc acgcccccaa acctgcaaac
     6361 ccagcagggg cgggggctgg cggggtgttg gaaaaatcca tccatgatta tctaagaata
     6421 atccactagg cgcggttatc agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc
     6481 cggccagagg ccggatagct ggtctattcg ctgcgctagg ctacacaccg ccccaccgct
     6541 gcgcggcagg gggaaaggcg ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat
     6601 acgctggagc gcttttagcc gctttagcgg cctttccccc tacccgaagg gtggggccgc
     6661 gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag cccggctcat ccttctggcg
     6721 tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc
     6781 catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac
     6841 cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg
     6901 ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc
     6961 cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat
     7021 catggaggca cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg
     7081 tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca
     7141 ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg
     7201 gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag
     7261 cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg
     7321 ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc
     7381 aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag
     7441 cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat
     7501 acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag
     7561 cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag
     7621 accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag
     7681 cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac
     7741 gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac
     7801 ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg
     7861 gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca
     7921 atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga
     7981 gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat
     8041 gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca
     8101 gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag
     8161 cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac
     8221 cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct
     8281 cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg
     8341 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
     8401 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
     8461 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
     8521 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
     8581 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
     8641 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     8701 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     8761 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     8821 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     8881 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     8941 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     9001 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     9061 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     9121 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     9181 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     9241 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     9301 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     9361 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
     9421 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
     9481 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
     9541 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
     9601 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
     9661 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
     9721 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
     9781 cagcgcctaa ccaccaactg cctgcaaagg aggcaatca
//
