LOCUS       sequence-432235-       13595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all 
            with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 13595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1561
FEATURES             Location/Qualifiers
     source          1..13595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3961..4627
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4497..4520
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      6291..6478
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6346..6370)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             9868..10671
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(11313..12128)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11313..12128)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12036..12055
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12120..12139
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      12353..12941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12842..12861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgtgtgg agtgtttgct tggattcttt agtaaaaggg
       61 gaagaacagt tggaagggcc aaagtggaag tcacaaaaca gtggtcctat ataaaagaac
      121 aagaaaaaga ttatttatat acaactgcgg tcacaagaag caacgcgaga gagcacaaca
      181 cgctgttatc acgcaaacta tgttttgaca ccgagccata gccgtgattg tgcgtcacat
      241 tgggcgataa tgaacgctaa atgaccaact cccatccgta ggagcccctt agggcgtgcc
      301 aatagtttca cgcgcttaat gcgaagtgct cggaacggac aactgtggtc gtttggcacc
      361 gggaaagtgg tactagaccg agagtttcgc atttgtatgg caggacgttc tgggagcttc
      421 gcgtctaaag ctttttcggg cgcgaaatgc agaccagacc agaacaaaac aactgacaag
      481 aaggcgttta atttaatatg ttgttcactc gcgcctgggc tgttgttatt cggctagata
      541 catacgtgtt tgtgcgtatg tagttatatc atatataagt atattaggat gaggcggtga
      601 aagagatttt ttttttttcg cttaatttat tcttttctct atcttttttc ctacatcttg
      661 ttcaaaagag tagcaaaaac aacaatcaat acaataaaat aagatctatg actgccgaca
      721 acaatagtat gccccatggt gcagtatcta gttacgccaa attagtgcaa aaccaaacac
      781 ctgaagacat tttggaagag tttcctgaaa ttattccatt acaacaaaga cctaataccc
      841 gatctagtga aacgtcaaat gacgaaagcg gagaaacatg tttttctggt catgatgagg
      901 agcaaattaa gttaatgaat gaaaattgta ttgttttgga ttgggacgat aatgctattg
      961 gtgccggtac caagaaagtt tgtcatttaa tggaaaatat tgaaaagggt ttactacatc
     1021 gtgcattctc cgtctttatt ttcaatgaac aaggtgaatt acttttacaa caaagagcca
     1081 ctgaaaaaat aactttccct gatctttgga ctaacacatg ctgctctcat ccactatgta
     1141 ttgatgacga attaggtttg aagggtaagc tagacgataa gattaagggc gctattactg
     1201 cggcggtgag aaaactagat catgaattag gtattccaga agatgaaact aagacaaggg
     1261 gtaagtttca ctttttaaac agaatccatt acatggcacc aagcaatgaa ccatggggtg
     1321 aacatgaaat tgattacatc ctattttata agatcaacgc taaagaaaac ttgactgtca
     1381 acccaaacgt caatgaagtt agagacttca aatgggtttc accaaatgat ttgaaaacta
     1441 tgtttgctga cccaagttac aagtttacgc cttggtttaa gattatttgc gagaattact
     1501 tattcaactg gtgggagcaa ttagatgacc tttctgaagt ggaaaatgac aggcaaattc
     1561 atagaatgct aggatccttg ggaagaggta gaagatccaa attgtaataa ctcgagtggc
     1621 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
     1681 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
     1741 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
     1801 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgc caaaccagat
     1861 gtcaacacag ctacaacgca cccatgaacc acacggttag tccaaaaggg gcagttcaga
     1921 ttccagatgc gggaattagc ttgctgccac cctcacctca ctaacgctgc ggtgtgcgga
     1981 tacttcatgc tatttataga cgcgcgtgtc ggaatcagca cgcgcaagaa ccaaatggga
     2041 aaatcggaat gggtccagaa ctgctttgag tgctggctat tggcgtctga tttccgtttt
     2101 gggaatcctt tgccgcgcgc ccctctcaaa actccgcaca agtcccagaa agcgggaaag
     2161 aaataaaacg ccaccaaaaa aaaaaaaata aaagccaatc ctcgaagcgt gggtggtagg
     2221 ccctggatta tcccgtacaa gtatttctca ggagtaaaaa aaccgtttgt tttggaattt
     2281 cccatttcgc ggccacctac gccgctatct ttgcaacaac tatctgcgat aactcagcaa
     2341 attttgcata ttcgtgttgc agtattgcga taatgggagt cttacttcca acataacggc
     2401 agaaagaaat gtgagaaaat tttgcatcct ttgcctccgt tcaagtatat aaagtcggca
     2461 tgcttgataa tctttctttc catcctacat tgttctaatt attcttattc tcctttattc
     2521 tttcctaaca taccaagaaa ttaatcttct gtcattcgct taaacactat atcaataaag
     2581 atctatggct tcagaaaaag aaattaggag agagagattc ttgaacgttt tccctaaatt
     2641 agtagaggaa ttgaacgcat cgcttttggc ttacggtatg cctaaggaag catgtgactg
     2701 gtatgcccac tcattgaact acaacactcc aggcggtaag ctaaatagag gtttgtccgt
     2761 tgtggacacg tatgctattc tctccaacaa gaccgttgaa caattggggc aagaagaata
     2821 cgaaaaggtt gccattctag gttggtgcat tgagttgttg caggcttact ggttggtcgc
     2881 cgatgatatg atggacaagt ccattaccag aagaggccaa ccatgttggt acaaggttcc
     2941 tgaagttggg gaaattgcca tctgggacgc attcatgtta gaggctgcta tctacaagct
     3001 tttgaaatct cacttcagaa acgaaaaata ctacatagat atcaccgaat tgttccatga
     3061 ggtcaccttc caaaccgaat tgggccaatt gatggactta atcactgcac ctgaagacaa
     3121 agtcgacttg agtaagttct ccctaaagaa gcactccttc atagttactt tcaagactgc
     3181 ttactattct ttctacttgc ctgtcgcatt ggccatgtac gttgccggta tcacggatga
     3241 aaaggatttg aaacaagcca gagatgtctt gattccattg ggtgaatact tccaaattca
     3301 agatgactac ttagactgct tcggtacccc agaacagatc ggtaagatcg gtacagatat
     3361 ccaagataac aaatgttctt gggtaatcaa caaggcattg gaacttgctt ccgcagaaca
     3421 aagaaagact ttagacgaaa attacggtaa gaaggactca gtcgcagaag ccaaatgcaa
     3481 aaagattttc aatgacttga aaattgaaca gctataccac gaatatgaag agtctattgc
     3541 caaggatttg aaggccaaaa tttctcaggt cgatgagtct cgtggcttca aagctgatgt
     3601 cttaactgcg ttcttgaaca aagtttacaa gagaagcaaa ggatccgggt ccttgggaag
     3661 aggtagaaga tccaaattgt agtaatggcg ccaattggtg cggcaattga taataacgaa
     3721 aatgtctttt aatgatctgg gtataatgag gaattttccg aacgttttta ctttatatat
     3781 atatatacat gtaacatata ttctatacgc tatagagaaa ggaaattttt caattaaaaa
     3841 aaaaatagag aaagagtttc acttcttgat tatcgctaac actaatggtt gaagtactgc
     3901 tactttaatt ttatgctgga tgcacacact ggcttaagat gacaacgcag ttcgagttta
     3961 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
     4021 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
     4081 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
     4141 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
     4201 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
     4261 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
     4321 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
     4381 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
     4441 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
     4501 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
     4561 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
     4621 aaacaaaaga tctatgatcg ctgccccatt agttagagat aacggttcag cattgaactt
     4681 cttcccacaa gccccacaag tagaaatcga tgagtcatca agaataatgg agttagttga
     4741 agcaaccagg agaactttaa gaaatgaatc atctgattct actgagaaaa tgagattgat
     4801 cgactctttg caaagattag gtttgaacca tcactttgaa caagatataa aagaaatgtt
     4861 acaggatttt gcaaacgaac acaagaatac caatcaagat ttgttcacta catctttgag
     4921 atttaggttg ttaagacaca atggtttcaa tgtaacacca gacgtcttca ataaatttac
     4981 cgaggaaaat ggtaagttca aggaatcatt aggtgaggat acaatcggaa tattgtcttt
     5041 atacgaagcc tcttatttag gaggtaaagg tgaagagatt ttatctgagg ctatgaagtt
     5101 ttctgaatct aaattgaggg aatcttctgg acacgtagca ccacatatca gaaggcaaat
     5161 atttcagtca ttggaattgc caaggcattt gaggatggct agattagaat caagaaggta
     5221 catcgaagaa gactactcta acgaaatcgg agccgattca tcattattag agttagctaa
     5281 attggatttc aattctgttc aagcattgca tcaaatggaa ttaactgaga ttagtagatg
     5341 gtggaagcag ttaggtttgt ctgataaatt acctttcgca agagacagac ctttggagtg
     5401 cttcttatgg actgtaggat tattaccaga accaaaatac tcaggttgca gaatcgagtt
     5461 ggccaagact attgctgttt tgttggttat tgacgacatc tttgacacct atggatctta
     5521 tgatcagttg atattattta caaacgctat aagacgttgg gatttagacg ccatggacga
     5581 gttgcctgaa tatatgaaaa tctgttacat ggcattatac aacactacaa atgagatatg
     5641 ttataaagtt ttgaaagaaa atggatggtc tgtattacct tacttagaga gaacctggat
     5701 tgacatggtt gagggtttta tgttagaggc taaatggtta aactcaggtg agcaaccaaa
     5761 tttagaagct tatatagaaa acggtgtaac cacagctgga tcatacatgg ctcttgttca
     5821 tctcttcttt cttataggtg acggagttaa tgatgaaaac gtaaaattgt tattggaccc
     5881 atatccaaag ttattctcat ctgccggtag aattttgaga ttatgggacg atttaggaac
     5941 tgcaaaggaa gagcaagaaa ggggtgacgt atcatcttca atccaattat acatgaagga
     6001 gaagaatgtt aggtcagaat ctgaaggaag ggagggtata gtcgagatta tttacaattt
     6061 gtggaaagat atgaatggtg agttaattgg ttctaatgct ttgccacaag ccattattga
     6121 gacatctttt aacatggcga gaacttctca agtagtctac caacacgagg atgacacata
     6181 cttttcttct gttgataatt acgtccaatc attgttcttc acaccagtat ctgtttctgt
     6241 tggatccggg tccttgggaa gaggtagaag atccaaattg tagtaatggc gcgaatttct
     6301 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     6361 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     6421 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     6481 tgccgagcaa atgcctgcaa atcgctcccc atttcgctgg ttctgtatgg gcacagacaa
     6541 cctaaacgaa gaggatgtcc aatatttttt ttaaggaata aggatacttc aagactagat
     6601 tcccccctgc attcccatca gaaccgtaaa ccttggcgct ttccttggga agtattcaag
     6661 aagtgccttg tccggtttct gtggctcaca aaccagcgcg cccgatatgg ctttcttttc
     6721 acttatgaat gtaccagtac gggacaatta gaacgctcct gtaacaatct ctttgcaaat
     6781 gtggggttac attctaacca tgtcacactg ctgacgaaat tcaaagtaaa aaaaaatggg
     6841 accacgtctt gagaacgata gattttcttt attttacatt gaacagtcgt tgtctcagcg
     6901 cgctttatgt tttcattcat acttcatatt ataaaataac aaaagaagaa tttcatattc
     6961 acgcccaaga aatcaggctg ctttccaaat gcaattgaca cttcattagc catcacacaa
     7021 aactctttct tgctggagct tcttttaaaa aagacctcag tacaccaaac acgttacccg
     7081 acctcgttat tttacgacaa ctatgataaa attctgaaga aaaaataaaa aaattttcat
     7141 acttcttgct tttatttaaa ccattgaatg atttcttttg aacaaaacta cctgtttcac
     7201 caaaggaaat agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag atctatggct
     7261 tcagaaaaag aaattaggag agagagattc ttgaacgttt tccctaaatt agtagaggaa
     7321 ttgaacgcat cgcttttggc ttacggtatg cctaaggaag catgtgactg gtatgcccac
     7381 tcattgaact acaacactcc aggcggtaag ctaaatagag gtttgtccgt tgtggacacg
     7441 tatgctattc tctccaacaa gaccgttgaa caattggggc aagaagaata cgaaaaggtt
     7501 gccattctag gttggtgcat tgagttgttg caggcttact tcttggtcgc cgatgatatg
     7561 atggacaagt ccattaccag aagaggccaa ccatgttggt acaaggttcc tgaagttggg
     7621 gaaattgcca tcaatgacgc attcatgtta gaggctgcta tctacaagct tttgaaatct
     7681 cacttcagaa acgaaaaata ctacatagat atcaccgaat tgttccatga ggtcaccttc
     7741 caaaccgaat tgggccaatt gatggactta atcactgcac ctgaagacaa agtcgacttg
     7801 agtaagttct ccctaaagaa gcactccttc atagttactt tcaagactgc ttactattct
     7861 ttctacttgc ctgtcgcatt ggccatgtac gttgccggta tcacggatga aaaggatttg
     7921 aaacaagcca gagatgtctt gattccattg ggtgaatact tccaaattca agatgactac
     7981 ttagactgct tcggtacccc agaacagatc ggtaagatcg gtacagatat ccaagataac
     8041 aaatgttctt gggtaatcaa caaggcattg gaacttgctt ccgcagaaca aagaaagact
     8101 ttagacgaaa attacggtaa gaaggactca gtcgcagaag ccaaatgcaa aaagattttc
     8161 aatgacttga aaattgaaca gctataccac gaatatgaag agtctattgc caaggatttg
     8221 aaggccaaaa tttctcaggt cgatgagtct cgtggcttca aagctgatgt cttaactgcg
     8281 ttcttgaaca aagtttacaa gagaagcaaa ggatccgggt ccggatccgg gtccggatcc
     8341 gggtccatgg cttcagaaaa agaaattagg agagagagat tcttgaacgt tttccctaaa
     8401 ttagtagagg aattgaacgc atcgcttttg gcttacggta tgcctaagga agcatgtgac
     8461 tggtatgccc actcattgaa ctacaacact ccaggcggta agctaaatag aggtttgtcc
     8521 gttgtggaca cgtatgctat tctctccaac aagaccgttg aacaattggg gcaagaagaa
     8581 tacgaaaagg ttgccattct aggttggtgc attgagttgt tgcaggctta cttcttggtc
     8641 gccgatgata tgatggacaa gtccattacc agaagaggcc aaccatgttg gtacaaggtt
     8701 cctgaagttg gggaaattgc catcaatgac gcattcatgt tagaggctgc tatctacaag
     8761 cttttgaaat ctcacttcag aaacgaaaaa tactacatag atatcaccga attgttccat
     8821 gaggtcacct tccaaaccga attgggccaa ttgatggact taatcactgc acctgaagac
     8881 aaagtcgact tgagtaagtt ctccctaaag aagcactcct tcatagttac tttcaagact
     8941 gcttactatt ctttctactt gcctgtcgca ttggccatgt acgttgccgg tatcacggat
     9001 gaaaaggatt tgaaacaagc cagagatgtc ttgattccat tgggtgaata cttccaaatt
     9061 caagatgact acttagactg cttcggtacc ccagaacaga tcggtaagat cggtacagat
     9121 atccaagata acaaatgttc ttgggtaatc aacaaggcat tggaacttgc ttccgcagaa
     9181 caaagaaaga ctttagacga aaattacggt aagaaggact cagtcgcaga agccaaatgc
     9241 aaaaagattt tcaatgactt gaaaattgaa cagctatacc acgaatatga agagtctatt
     9301 gccaaggatt tgaaggccaa aatttctcag gtcgatgagt ctcgtggctt caaagctgat
     9361 gtcttaactg cgttcttgaa caaagtttac aagagaagca aaggatccta actcgaggcg
     9421 atttaatctc taattattag ttaaagtttt ataagcattt ttatgtaacg aaaaataaat
     9481 tggttcatat tattactgca ctgtcactta ccatggaaag accagacaag aagttgccga
     9541 cagtctgttg aattggcctg gttaggctta agtctgggtc cgcttcttta caaatttgga
     9601 gaatttctct taaacgatat gtatattctt ttcgttggaa aagctgagca gttacagaga
     9661 tgttacgaac cactagtgca ctgcagtaca cggtttcctt gaaatttttt tgattcggta
     9721 atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc
     9781 atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca
     9841 aaaacgtgca ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct
     9901 actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac
     9961 ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta
    10021 ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag
    10081 ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac
    10141 agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga
    10201 atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc
    10261 ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca
    10321 gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt
    10381 gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga
    10441 gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagat
    10501 gcattgggtc aacagtatag aaccgtggat gatgtggttt ctacaggatc tgacattatt
    10561 attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac
    10621 agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta
    10681 ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag
    10741 ttattacccg agtagagcac ttgaatccac tgccccggga atctcggtcg taatgatttc
    10801 tataatgacg aaaaaaaaaa aattggaaag aaaaagcttc atggccttta taaaaaggaa
    10861 ctatccaata cctcgccaga accaagtaac agtattttac ggggcacaaa tcaagaacaa
    10921 taagacagga ctgtaaagat ggacgcattg aactccaaag aacaacaaga gttccaaaaa
    10981 gtagtggaac aaaagcaaat gaaggatttc atgcgtttgt actctaatct ggtagaaaga
    11041 tgtttcacag actgtgtcaa tgacttcaca acatcaaagc taaccaataa ggaacaaaca
    11101 tgcatcatga agtgctcaga aaagttcttg aagcatagcg aacgtgtagg gcagcgtttc
    11161 caagaacaaa acgctgcctt gggacaaggc ttgggccgat aaggtgtact ggcgtatata
    11221 tatctaatta tgtatctctg gtgtagccca tttttagcat gtaaatataa agaccgagcg
    11281 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
    11341 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
    11401 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
    11461 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
    11521 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
    11581 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
    11641 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
    11701 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
    11761 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
    11821 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
    11881 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
    11941 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
    12001 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
    12061 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
    12121 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
    12181 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
    12241 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
    12301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    12361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    12421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    12481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    12541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    12601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    12661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    12781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    13021 ctgattctgt ggataaccgt gcggccgcca atgctaaatt cgagtgaaac acaggaagat
    13081 cagaaaatcc tcatttcatc catattaaca ataatttcaa atgtttattt gcattatttg
    13141 aaactaggca agacaagcaa cgaaacgttt ttgaaaattt tgagtatttt caataaattt
    13201 gtagaggact cagatattga aaaaaagcta cagcaattaa tacttgataa gaagagtatt
    13261 gagaagggca acggttcatc atctcatgga tctgcacatg aacaaacacc agagtcaaac
    13321 gacgttgaaa ttgaggctac tgcgccaatt gatgacaata cagacgatga taacaaaccg
    13381 aagttatctg atgtagaaaa ggattaaaga tgctaagaga tagtgatgat atttcataaa
    13441 taatgtaatt ctatatatgt taattacctt ttttgcgagg catatttatg gtgaaggata
    13501 agttttgacc atcaaagaag gttaatgtgg ctgtggtttc agggtccata aagcccacat
    13561 ggataacatt acccctgaat tcgcatctag actga
//
