LOCUS       sequence-432240-        2323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express separately the Rotavirus SA11 NSP3 protein and the Rotavirus
            SA11 NSP5 fused with SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2323)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP3SA11-P2A-NSP5(wt)-SNAP
FEATURES             Location/Qualifiers
     source          1..2323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             874..930
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1555..2100
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gctgcaacct cagctcttga gaatatggga atagaatatg attatcagga tatatattct
       61 agagtaaaga ataaatttga ttttgtgatg gacgattctg gtgttaaaaa taatctgatt
      121 ggtaaagcaa taactattga tcaagctttg aataataaat ttggatctgc tataagaaat
      181 agaaactggc ttgctgatac ttctagagca gctaaattag atgaggatgt aaacaaacta
      241 agaatgatgt tatcatcaaa aggaattgat caaaaaatga gagttttaaa cgcatgcttc
      301 agtgtaaaaa gaatacctgg aaaatcatca tctattatta aatgcacaaa attgatgcgt
      361 gataaattgg aacgtggtga agttgaagtg gatgattcat ttgtggatga aaaaatggaa
      421 gtggatacca ttgactggaa atcgcgctat gagcaattgg agcaaaggtt tgaatcattg
      481 aaatccaggg taaatgaaaa atataataat tgggtgttga aagcaagaaa aatgaatgaa
      541 aatatgcatt ctcttcaaaa tgtcatctct caacagcaag cacatatagc tgagcttcaa
      601 gtgtacaata ataaactaga acgtgatttg caaaataaaa ttggatccct tacttcttcg
      661 attgaatggt atttaagatc aatggaatta gaccctgaaa taaaggcaga cattgaacag
      721 caaattaact caattgatgc gataaatcca ttgcacgctt ttgatgactt agaatcagta
      781 atacgtaatt tgatatctga ttatgacaaa ttattcctta tgttcaaagg attaatacag
      841 agatgtaatt atcaatattc atttggttgc gaagcaacga acttctctct gttaaagcaa
      901 gcaggagacg tggaagaaaa ccccggtcct atgtcactta gtatagacgt tacttcctta
      961 ccttccatac ctagtacaat atataagaat gaaagtagta gtacaacaag taccctaagt
     1021 ggaaaaagta taggaagaag tgaacaatat ataagcccag acgccgaagc gttcaataaa
     1081 tacatgttat ccaaatcccc cgaagatatc ggaccctcag actccgcatc aaatgatcct
     1141 ttaacaagtt tttcaatccg aagtaacgca gtgaaaacaa atgccgatgc aggtgtaagc
     1201 atggacagtt ctgcacaatc aagaccttca tctaacgtag gctgcgatca agtagatttt
     1261 tcattaaata aaggcttaaa agtaaaagcc aatttagata gtagtatatc tataagcaca
     1321 gatacaaaaa aagagaaatc aaaacaaaac cataaaagta ggaaacatta tccaagaata
     1381 gaagcagaaa gtgattctga cgattatgta ttagacgata gcgattcaga tgatggaaaa
     1441 tgtaaaaact gtaaatataa gaagaaatat ttcgcactaa ggatgagaat gaaacaagtt
     1501 gccatgcaac ttatagaaga tttaggttct ggatccggtt ctggaagtgg atccatggac
     1561 aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct ggaactgtct
     1621 gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc tgccgccgac
     1681 gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact gatgcaggcc
     1741 accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca
     1801 gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct gtggaaactg
     1861 ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggccgc cctggccggc
     1921 aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt gcccattctg
     1981 atcccctgcc accgggtggt gcagggcgac ctggacgtgg ggggctacga gggcgggctc
     2041 gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc tgggctgggt
     2101 taaccatttt gatacatgtt gaacaatcaa atacagtgtt agtatgttgt catctatgca
     2161 taaccctcta tgagcacaat agttaaaagc taacactgtc aaaaacctaa atggctatag
     2221 gggcgttatg tggccgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat
     2281 ccgaaggagg acgtcgtcca ctcggatggc taagggagag ctc
//
