LOCUS       sequence-432242-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the Rotavirus SA11 delta103C NSP1 protein fused with the 
            sfGFP (Pedelacq et al., 2006).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP1delta103CSA11-GFP
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..786
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     YFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     DVTAAGITHGMDELYK"
     primer_bind     complement(103..131)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttaattaaaa gattaatttg gagtttgttt actaatcaca tggatcaaac aattgaagaa
       61 gtgtttaatc acatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      121 gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat
      181 gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg
      241 tggccaacac ttgtcactac tctgacctat ggtgttctat gcttttcccg ttatccggat
      301 cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc
      361 actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt
      421 gatacccttg ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      481 ctcggacaca aactggagta caactttaac tcacacaatg tatacatcac ggcagacaaa
      541 caaaagaatg gaatcaaagc gtacttcaaa attcgccaca acgttgaaga tggttccgtt
      601 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      661 gacaaccatt acctctcgac acaatctgtt ctttcgaaag atcccaacga aaagcgtgac
      721 cacatggtcc ttcttgagga tgtaactgct gctgggatta cacatggcat ggatgaactc
      781 tacaaatgaa attatgtcac tatctaatta tacagtattt agccatcaca agaccgtcca
      841 gactagagta gcgcctagct ggcaaaatac tgtgaaccgg gtcggcatgg catctccacc
      901 tcctcgcggt ccgacctggg catccgaagg aggacgtcgt ccactcggat ggctaaggga
      961 gagctc
//
