LOCUS       sequence-432251-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that 
            lacks all methionines fused with the full-length SA11 NSP5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1noMSA11-NSP5(wt)
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(195..206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atatcgatcc cgggttaata cgactcacta tagggctttt ttttgaaaag tcttgtgtta
       61 gccaaggcta cttttaaaga agcaagcttt cattatcgta gattaactgc tttaaatcgg
      121 agattaagca acattggtgc aaattctatt tggaagccag ttcctgccat gtctctcagt
      181 attgacgtga cgagtcttcc ttctattcct tcaactatat ataagaatga atcgtcttca
      241 acaacgtcaa ctctttctgg aaaatctatt ggtaggagtg aacagtacat ttcaccagat
      301 gcagaagcat tcaataaata catgctgtcg aagtctccag aggatattgg accatctgat
      361 tctgcttcaa acgatccact caccagtttt tcgattagat cgaatgcagt taagacaaac
      421 gcagacgctg gcgtgtctat ggattcatca gcacaatcac gaccttcaag taatgtcgga
      481 tgcgatcaag tggatttctc cttaaataaa ggcttaaaag taaaagctaa tttggactca
      541 tcaatatcaa tatctacgga tactaaaaag gagaaatcaa aacaaaacca taaaagtagg
      601 aagcactacc caagaattga agcagagtct gattcagatg attatgtact ggatgattca
      661 gatagtgatg atggtaaatg taagaactgt aaatataaga agaaatactt cgcattaaga
      721 atgagaatga aacaagtcgc aatgcaattg attgaagatt tgtaaaatta tgtcactatc
      781 taattataca gtatttagcc atcacaagac cgtccagact agagtagcgc ctagctggca
      841 aaatactgtg aaccgggtcg gcatggcatc tccacctcct cgcggtccga cctgggcatc
      901 cgaaggagga cgtcgtccac tcggatggct aagggagagc tcaaaaaaaa ggatccggct
      961 g
//
