LOCUS       sequence-432253-        1447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express separately the short Rotavirus SA11 NSP1 protein (35 amino 
            acids) and the full-length recoded NSP5 protein fused with GS-linker
            and smURFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1447)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP
FEATURES             Location/Qualifiers
     source          1..1447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             169..225
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             850..1251
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
ORIGIN
        1 atatcgatcc cgggttaata cgactcacta tagggctttt ttttgaaaag tcttgtgtta
       61 gccatggcta cttttaaaga tgcatgcttt cattatcgta gattaactgc tttaaatcgg
      121 agattatgca acattggtgc aaattctatt tggatgccag ttcctgatgc tactaacttc
      181 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgag tttaagtata
      241 gatgttacat cacttccctc gatcccctca acaatatata aaaatgaaag ttcaagtaca
      301 acaagtacac tatccggaaa atctatagga agaagtgaac aatatataag cccagatgca
      361 gaagctttta ataaatatat gctaagtaaa tcacctgaag atatagggcc aagtgatagt
      421 gcatcaaacg atcccctcac aagtttttca atacgaagta acgcagtaaa aactaatgcc
      481 gatgcaggtg taagcatgga ctcctcagca caatcaagac cttcatctaa cgtaggatgt
      541 gatcaagtcg atttctcatt aaacaaagga ttaaaagtta aagcaaattt agattcatct
      601 ataagtataa gcacagatac aaaaaaagaa aaaagtaaac aaaatcataa aagtcgaaaa
      661 cactatccaa gaatagaagc cgaaagtgat agcgatgatt atgtactaga cgacagcgat
      721 tcagatgatg gaaaatgtaa aaattgcaaa tacaaaaaaa aatattttgc actaagaatg
      781 agaatgaaac aagttgccat gcaattaatt gaagatttag gttctggatc cggttctgga
      841 agtggatcca tggctaagac ttccgaacag agggtgaaca ttgctacact gctgacagaa
      901 aataagaaga aaatcgtgga taaggcttcc caggatctgt ggcggagaca cccagacctg
      961 atcgcaccag gaggaattgc tttctctcag agggaccgcg ctctgtgcct gcgagattac
     1021 ggctggttcc tgcatctgat caccttttgt ctgctggccg gagataaggg ccccatcgag
     1081 tctattgggc tgatcagtat tcgagaaatg tataactcac tgggagtgcc cgtccctgca
     1141 atgatggaga gcattagatg cctgaaagaa gccagcctgt ccctgctgga cgaagaggac
     1201 gccaacgaga ccgcacccta ctttgattac attattaagg ctatgagcta aaattatgtc
     1261 actatctaat tatacagtat ttagccatca caagaccgtc cagactagag tagcgcctag
     1321 ctggcaaaat actgtgaacc gggtcggcat ggcatctcca cctcctcgcg gtccgacctg
     1381 ggcatccgaa ggaggacgtc gtccactcgg atggctaagg gagagctcaa aaaaaaggat
     1441 ccggctg
//
