LOCUS       sequence-432255-        7485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1505
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1853..2040
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1908..1932)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2516..2923
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2924..4018
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5049..5864)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5049..5864)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5772..5791
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5856..5875
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6089..6677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6578..6597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga aacacaatcg
      721 tccaaatggt actgggaaag cagtttctgg cttcaagcag ataataagaa ggcttctact
      781 cctcttgaac aagaagagaa gaaaacaact cgtcatcatc ttgaaaagaa taacccaagt
      841 ctatggaata aacctagtat tttatgtcaa aaaatggaaa ttgaaaaaat tacagggtga
      901 aaatattcaa ataaatgata tcatgccatg gctaagagag tctactatat tagtcttgct
      961 gaacatacta tatccaactt tgatgaagtt cccattttta aaaaatcatt atattcactg
     1021 gtcttccatt gtaggaatat cgttaatgct taccaagggt gaggtacctt cgtggattat
     1081 cgcacacttt cttgtggaag ctatagcctc caaattaaag atcgcaaaat taacacaatg
     1141 gttgaaaaaa aaaaattttt cccagggtac attaataaaa ttccagcaaa ttcttgtgtg
     1201 tttagcgatt attgtattat ttgccaaact ggacaggagt tcgttaccat tccgtgtgct
     1261 ttttgaccat aggccatttt taatagattt cttcacaatc aatgccattt tcacagtttt
     1321 ggcagtatat cacagaacat taaaattctt ttttacttca gggacgaaat caaataagaa
     1381 tgtcggcggc cacgaagtca ggaacttttc tcaatacctt ggggtcaaga accataatga
     1441 ttggcccata tcttcatcaa atttgaagca tgtaatggac agactaaatg aaatacatga
     1501 agttacaatt gatgataatt atgcaaatat aaacgaaaag ataataaatt catatttcac
     1561 caagggtttt tttccaagtc taaaatggac tatattaagg cagtgtatcg aatatttatt
     1621 cgtcacgaaa aggcgcagat taatgggcaa caaattgcgg tgcatcgtga tgcttcttac
     1681 attcaccttc gtggatccaa caagtaaaat gaaaatcagc ccatttttcg caaaattctt
     1741 tgcgaagtcc ttggttaatg tttacttgaa aaagtattgg cattgcaact ttgggaaata
     1801 tatactattc tttttgtttc agcttagtat tatgtgagga tcctaactcg aggcgaattt
     1861 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     1921 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     1981 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     2041 catgccgagc aaatgcctgc aaatcgctcc ccatttcgct ggaaatctgc tcgtcagtgg
     2101 tgctcacact gacgaatcat gtacagatca taccgatgac tgcctggcga ctcacaacta
     2161 agcaagacag ccggaaccag cgccggcgaa caccactgca tatatggcat atcacaacag
     2221 tccacgtctc aagcagttac agagatgtta cgaaccacta gtgcactgca gtacatcgag
     2281 gagaacttct agtatatcta catacctaat attattgcct tattaaaaat ggaatcccaa
     2341 caattacatc aaaatccaca ttctcttcaa aatcaattgt cctgtacttc cttgttcatg
     2401 tgtgttcaaa aacgttatat ttataggata attatactct atttctcaac aagtaattgg
     2461 ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat atcttgaccg cagttaactg
     2521 tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt
     2581 ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg
     2641 gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt
     2701 gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt
     2761 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     2821 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     2881 ttcagcaata tatatatata tatttcaagg atataccatt ctaatgtctg cccctaagaa
     2941 gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag ccattaaggt
     3001 tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat
     3061 tggtggtgct gctatcgatg ctacaggtgt tccacttcca gatgaggcgc tggaagcctc
     3121 caagaaggct gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg gaaccggtag
     3181 tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt acgccaactt
     3241 aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca agccacaatt
     3301 tgctaaaggt actgacttcg ttgttgtcag agaattagtg ggaggtattt actttggtaa
     3361 gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca ccgttccaga
     3421 agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc caccattgcc
     3481 tatttggtcc ttggataaag ctaatgtttt ggcctcttca agattatgga gaaaaactgt
     3541 ggaggaaacc atcaagaacg aatttcctac attgaaggtt caacatcaat tgattgattc
     3601 tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa tcaccagcaa
     3661 catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt
     3721 gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc
     3781 atgccacggt tctgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt
     3841 gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta aggccattga
     3901 agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa
     3961 cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa
     4021 agattctctt tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa
     4081 cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct
     4141 acatacattt atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg
     4201 atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag
     4261 gaggagggca ccacacaaaa agttaggtgt aacagaaaat catgaaacta tgattcctaa
     4321 tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa
     4381 tgacctgacc atttgatgga gtttaagtca ataccttctt gaaccatttc ccataatggt
     4441 gaaagttccc tcaagaattt tactctgtca gaaacggcct taacgacgta gtcgagagta
     4501 ctcgtatcgc atgtcggtgc gacacgaaat tacaaaatgg aatatgttca tagggtagac
     4561 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg
     4621 aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt
     4681 taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc
     4741 atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca
     4801 acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg
     4861 aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt
     4921 aacgacgtag tcgacctcct cttcagtact aaatctacca ataccaaatc tgatggaaga
     4981 atgggctaat gcatcatcct tacccagcgc cgagcggccg cgtgttacaa ccaattaacc
     5041 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     5101 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     5161 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     5221 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     5281 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     5341 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     5401 cgtgattgcg cctgagcgag gcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     5461 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     5521 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     5581 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     5641 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     5701 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     5761 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     5821 aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     5881 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     5941 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     6001 ttgaaggatc agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     6781 ccgccaatac ccagaagaac agtaaaataa agcaaggtac gtgaaattaa tatttttaaa
     6841 tggttctaac cgatgccgaa gaactgcgca gtccggttat aacgtctgac atgtcctttt
     6901 ttgatttgga atccaaccac tcaagtgact ctgttcattt actttgcgaa aaatataccc
     6961 acaaattgcc catcgaaagt gaatcgcaaa ccaccttcag actggcaccg acaaagcaaa
     7021 gattatacag acagagtact ttatacgtac cgttaagtct caagcaaagg gttttcttat
     7081 ttactgaacg ggtaaagagt atctgggccg gcttgccaag atgcaaaccg aataagtatt
     7141 tcaaagttgc atttgcctta gccgtcctga caccattggc tatttggata ttttatattg
     7201 actttcgtgt acattgatca catcgactgt tctattggca aatgaaccac gggcattgac
     7261 tatttttcag gttactacta tatattatca tcacgggcaa ggattgtacc ctgaattcgc
     7321 atctagactg atgagacgtg gtagagccac aaacagccgg tacaagcaac gatctccagg
     7381 accatctgaa tcatgcgcgg atgacacgaa ctcacgacgg cgatcacaga cattaaccca
     7441 cagtacagac actgcgacaa cgtggcaatt cgtcgcaata caacg
//
