LOCUS       sequence-432266-        8019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex29.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1502
FEATURES             Location/Qualifiers
     source          1..8019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2170..2181
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2387..2574
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2442..2466)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3050..3457
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3458..4552
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5583..6398)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5583..6398)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6306..6325
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6390..6409
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6623..7211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7112..7131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg actcggtgac
      721 aaattttttc tggaatgaca cctataatgc tggtactcca actagatcaa ctctcaaggg
      781 gaagaaagtg caaaatggca tagatggaaa aagtcaggca aagaaagaga gcatatctag
      841 tggttctaga actagtgatc ctacacgtgg tagtctgcca tcttccagtg gacagccaac
      901 tagtggtggc ggttttccct cgacatcaaa catccaaaag atgatggcag ataccctggt
      961 agagaagata atcaaaatgg cattaccacc atcttccaaa acggctgttg atacaattca
     1021 ccatcgtatg gttgctggta aagaaaggcc taagctgtct gtgcagatca cgagtaggaa
     1081 ttttatacag atgaactcga gactaggggt accattcatg attatggatg aattaataaa
     1141 gatattaaac tggactaacc cagcgtatac agtatctatc atgttcttat acacgctcat
     1201 tatattgaaa ccttttcaaa tgctttcttc gctccctata ttctatttgt tattctgcgt
     1261 aatggtcccg caataccttt acattcataa gccaaaccct acaagctatc tagataataa
     1321 tcaaacacca gcacaaggcc cacctttgcg aagacctgag gttcctaaac cagtccctga
     1381 actgagccaa gaatttgtct taaacctcac ggatttacaa aaccatatgc tgctatatgt
     1441 taaattctac gattttactt tactcatatt acaaaagttt gcattcttca ccaacgaggc
     1501 tatttcgtcg ttttatttta ttgtattgtt aataatagca acattaaatt ttctgtacat
     1561 ggataaattt atcaagctca ttccaatgag gcccgtactg atactgctag gttggggttt
     1621 ttttattgcc tcgcatccgt ccaatcgtga atatttgttg actaaattga attcggagga
     1681 gacaagatta aaaactttga ctataagtac taatctggaa tctaagattt tgcagcattt
     1741 gaaactcatt gaggctaggg agcaccgact tgttatgatc tttgagatcc aaaagtatct
     1801 tcctgaatac aaagaatggc gcccagttgg attctctgat gacgattatt ctcttttctc
     1861 tagtttgaga atataccaga ggcgtattga ggaaaattca gtgaagtcat tagaagaaat
     1921 tgagccacct aaggattggg aatgggaagc caattctcat tgggtgcttg atttggatcc
     1981 aaaagaatgg gtagaggacg aatttattca gtatgtcgag atagattcag aaacaaaatg
     2041 ggtctatgac ctcaatcttg atggacaaag gggctcatat agaaggcgaa tgtggaccaa
     2101 cagttgcgtg cggaaaaaac tggattctgg tatttcttca aaccttggtg aggaggaagt
     2161 agttaatcca ttgagggaag aaacatatag gcaaggcgta catggcgtga cgaagggttc
     2221 aatgagtggc ggtctaactc acagtagtga tgatgaccgc gctgatgagg aaagtataaa
     2281 cggtactatt cctaatctca acaatatcga cgcggatgca tcttatccgt caatcgaaga
     2341 gctaacagac actctcaatt caactatata aggatcctaa ctcgaggcga atttcttatg
     2401 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     2461 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     2521 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     2581 gagcaaatgc ctgcaaatcg ctccccattt cgctggaaat ctgctcgtca gtggtgctca
     2641 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     2701 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     2761 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacat cgaggagaac
     2821 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta
     2881 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt
     2941 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt
     3001 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa
     3061 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc
     3121 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
     3181 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
     3241 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
     3301 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
     3361 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
     3421 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt
     3481 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa
     3541 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg
     3601 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa
     3661 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggaaccg gtagtgttag
     3721 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc
     3781 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa
     3841 aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa
     3901 ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca
     3961 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg
     4021 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga
     4081 aaccatcaag aacgaatttc ctacattgaa ggttcaacat caattgattg attctgccgc
     4141 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt
     4201 tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc
     4261 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca
     4321 cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc
     4381 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc
     4441 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac
     4501 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc
     4561 tcttttttta tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac
     4621 gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac
     4681 atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta
     4741 atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag
     4801 ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata
     4861 tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct
     4921 gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt
     4981 tccctcaaga attttactct gtcagaaacg gccttaacga cgtagtcgag agtactcgta
     5041 tcgcatgtcg gtgcgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
     5101 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
     5161 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
     5221 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
     5281 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
     5341 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
     5401 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
     5461 gtagtcgacc tcctcttcag tactaaatct accaatacca aatctgatgg aagaatgggc
     5521 taatgcatca tccttaccca gcgccgagcg gccgcgtgtt acaaccaatt aaccaattct
     5581 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     5641 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     5701 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     5761 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     5821 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
     5881 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     5941 tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     6001 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     6061 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     6121 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     6181 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     6241 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     6301 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     6361 ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     6421 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     6481 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     6541 gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgcca
     7321 atacccagaa gaacagtaaa ataaagcaag gtacgtgaaa ttaatatttt taaatggttc
     7381 taaccgatgc cgaagaactg cgcagtccgg ttataacgtc tgacatgtcc ttttttgatt
     7441 tggaatccaa ccactcaagt gactctgttc atttactttg cgaaaaatat acccacaaat
     7501 tgcccatcga aagtgaatcg caaaccacct tcagactggc accgacaaag caaagattat
     7561 acagacagag tactttatac gtaccgttaa gtctcaagca aagggttttc ttatttactg
     7621 aacgggtaaa gagtatctgg gccggcttgc caagatgcaa accgaataag tatttcaaag
     7681 ttgcatttgc cttagccgtc ctgacaccat tggctatttg gatattttat attgactttc
     7741 gtgtacattg atcacatcga ctgttctatt ggcaaatgaa ccacgggcat tgactatttt
     7801 tcaggttact actatatatt atcatcacgg gcaaggattg taccctgaat tcgcatctag
     7861 actgatgaga cgtggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc
     7921 tgaatcatgc gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac
     7981 agacactgcg acaacgtggc aattcgtcgc aatacaacg
//
