LOCUS       sequence-432267-        7743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1504
FEATURES             Location/Qualifiers
     source          1..7743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2111..2298
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2166..2190)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2774..3181
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3182..4276
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5307..6122)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5307..6122)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6030..6049
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6114..6133
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6347..6935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6836..6855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga gcgaaataaa
      721 taatgaaaat ctagagccaa cctcaagtac agtagctgag tctacagaaa gcaagaataa
      781 acacattcgt agcgctttaa gaaaacgaag aggtaaatta agcgcccaaa cttatgagga
      841 agatcaagag gctattttat catctccttt attgacttca acacctaaaa cagtctccag
      901 gtcattagtg aggttatacc catatttaat tgttgtggat aactttctaa gtatcatcac
      961 ttggtccaat gacaatgtct ctgcaaactt gcttggaata ttcttattta cggtttgtgt
     1021 tctatatttt ggatttatta caaggtattt tggacattta atgatcgttg gtataatatg
     1081 ggtatatctt ttaattgaca agcacgttca agaaacaatg gcatcttgcc cttcattgga
     1141 tgatataata cacgttatgg atagggtttc tatgaaatct agcgctgtgt tatctcctat
     1201 tacgattttg agcgctcagg atgttagaag actgttattt accattgcct tcctatcccc
     1261 agtttatatt tttctaacag tgtttgtgct atctccaaac tatttaatgt taataggggg
     1321 tctgtatgtg cttacgtacc attctaaact aatccgaaga atgagaagat acttgtggaa
     1381 atttcgcgtt gttagattac ttgtattttt cataacagga ttagaccttg gtggaccaga
     1441 taataatagg cgcttgtttg cttctgtcaa taagaaaata aggtcctttg tttggaatga
     1501 ggtagggaac acatctaaca ctaaaaagac tgtacttttc aaggttgctc tgtttgaaaa
     1561 ccagcgccgc tggcttggca tcggatggac atcaactatg ttgagttatg agcgcgcatc
     1621 ttggacagat gaattcctaa atacttctcc aagcccagaa gtgtttactt taccagagga
     1681 gcagtcggga atggcttggg aatggcacga taaagattgg atgcttgacc taacaaatga
     1741 tgggatcatt caacttcctg cttctgctgc taagacaaaa gttaagccag gtgcagatga
     1801 aggtttcata tattatgata acacctggaa taatccctct gcaacggaca catataaaaa
     1861 gtacacaaga agaaggaggt ggatcaggac tgcaacagta acaacaacgt atgacgatga
     1921 gcccactgta gaaaaagcta caccgaattc tcatgctctg aagagtgaag aaaataatag
     1981 agttagaaaa agaaaagtgt catttagtac agcaaatgaa gtgcatatca taccttcctc
     2041 ggacagtagc aaattaatac aaatatctga tgtttcaatg tctccttctc tataaggatc
     2101 ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     2161 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     2221 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     2281 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctgg
     2341 aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata ccgatgactg
     2401 cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca ccactgcata
     2461 tatggcatat cacaacagtc cacgtctcaa gcagttacag agatgttacg aaccactagt
     2521 gcactgcagt acatcgagga gaacttctag tatatctaca tacctaatat tattgcctta
     2581 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
     2641 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
     2701 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
     2761 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     2821 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     2881 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     2941 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
     3001 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     3061 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     3121 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
     3181 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
     3241 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
     3301 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
     3361 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
     3421 taaatgggga accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
     3481 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
     3541 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
     3601 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
     3661 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
     3721 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
     3781 attatggaga aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaggttca
     3841 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     3901 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     3961 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     4021 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
     4081 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     4141 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     4201 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     4261 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     4321 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     4381 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     4441 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     4501 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     4561 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     4621 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
     4681 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     4741 acgacgtagt cgagagtact cgtatcgcat gtcggtgcga cacgaaatta caaaatggaa
     4801 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     4861 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     4921 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     4981 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     5041 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     5101 taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac
     5161 tctgtcagaa acggccttaa cgacgtagtc gacctcctct tcagtactaa atctaccaat
     5221 accaaatctg atggaagaat gggctaatgc atcatcctta cccagcgccg agcggccgcg
     5281 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     5341 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     5401 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     5461 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     5521 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     5581 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     5641 accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta
     5701 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     5761 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     5821 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     5881 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     5941 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     6001 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     6061 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
     6121 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     6181 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     6241 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
     6301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7021 ctgtggataa ccgtgcggcc gccaataccc agaagaacag taaaataaag caaggtacgt
     7081 gaaattaata tttttaaatg gttctaaccg atgccgaaga actgcgcagt ccggttataa
     7141 cgtctgacat gtcctttttt gatttggaat ccaaccactc aagtgactct gttcatttac
     7201 tttgcgaaaa atatacccac aaattgccca tcgaaagtga atcgcaaacc accttcagac
     7261 tggcaccgac aaagcaaaga ttatacagac agagtacttt atacgtaccg ttaagtctca
     7321 agcaaagggt tttcttattt actgaacggg taaagagtat ctgggccggc ttgccaagat
     7381 gcaaaccgaa taagtatttc aaagttgcat ttgccttagc cgtcctgaca ccattggcta
     7441 tttggatatt ttatattgac tttcgtgtac attgatcaca tcgactgttc tattggcaaa
     7501 tgaaccacgg gcattgacta tttttcaggt tactactata tattatcatc acgggcaagg
     7561 attgtaccct gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta
     7621 caagcaacga tctccaggac catctgaatc atgcgcggat gacacgaact cacgacggcg
     7681 atcacagaca ttaacccaca gtacagacac tgcgacaacg tggcaattcg tcgcaataca
     7741 acg
//
