LOCUS       sequence-432289-       10745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCas9 for CRISPR-interference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
  TITLE     A series of vectors for inducible gene expression in 
            multidrug-resistant Acinetobacter baumannii.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 
            10.1128/aem.00474-24. Epub 2024 Aug 20.
  PUBMED    39162403
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-09-18"; volume: "90(9):e0047424. 
            doi"; pages: " 10.1128/aem.00474-24. Epub 2024 Aug 20"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-hph-dCas9
FEATURES             Location/Qualifiers
     source          1..10745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  28..193
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            352..460
                     /note="incP origin of transfer"
     rep_origin      complement(692..1280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(772..791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1451..2242,2243..2311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2074..2093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2312..2416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2484..2502
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2540..2562)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2662..2681
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3095..3111
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3096..3112
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3140..3234
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3337..3423
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(3477..3524)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3607..3981)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        4001..4105
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(5358..5405)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    complement(5516..5563)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(5601..6227)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     6132..6151
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        6243..6298
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    6279..6297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             6316..6327
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(6334..6360,6361..6363,6364..8850,8851..8853,
                     8854..10440)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      10464..10535
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10551..10578
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cggtacctcg cgaaggcctt gcaggccaac cagataagtg aaatctagtt ccaaactatt
       61 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
      121 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
      181 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tgggcgctgc attaatgaat
      241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      301 tgacccgctg cgctcggtcg ttcggctgcg gcgagcggta tcagagctta tcggccagcc
      361 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      421 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      481 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      541 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaagtatacc
      601 ttaaggaatc ccctgataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      721 atcacaaaaa tcgacgctcg agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2461 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     2521 tcggtgacga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2641 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     2701 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     2761 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     2821 tagaggacca gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc
     2881 tgcgtaagca ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
     2941 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     3001 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     3061 aaattgcccg tcgtattaaa gaggggcgtg gggttcgagg tcgacggtat cgataagcta
     3121 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     3181 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     3241 tagactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
     3301 aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg
     3361 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     3421 aatccgccgc taggagcttg cggcccggac gatcgaatta gcttcaaaag cgctctgaag
     3481 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaagatc ccctgattcc
     3541 ctttgtcaac agcaatggat ttatctctga tgttacattg cacaagataa aaatatatca
     3601 tcatgatcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag
     3661 ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc
     3721 ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac
     3781 ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag
     3841 ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc
     3901 gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac
     3961 ggcactggtc aacttggcca tgaaagcatg cggaaatgtg cgcggaaccc ctatttgttt
     4021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4081 tcaataatat tgaaaaagga agagtatgag ttgaaaattt tttttttgac tcaatatcta
     4141 gacttgcaag agcttggaac tttgagattg ttctaagatg catacaaatt caaaatgctt
     4201 aaacaaaaat aatttaacaa aggagtaaga ttcatatgac acaagaatcc ctgttacttc
     4261 tcgaccgtat tgattcggat gattcctacg cgagcctgcg gaacgaccag gaattctggg
     4321 agccgctggc ccgccgagcc ctggaggagc tcgggctgcc ggtgccgccg gtgctgcggg
     4381 tgcccggcga gagcaccaac cccgtactgg tcggcgagcc cggcccggtg atcaagctgt
     4441 tcggcgagca ctggtgcggt ccggagagcc tcgcgtcgga gtcggaggcg tacgcggtcc
     4501 tggcggacgc cccggtgccg gtgccccgcc tcctcgtccg cggcgagctg cggcccggca
     4561 ccggagcctg gccgtggccc tacctggtga tgagccggat gaccggcacc acctggcggt
     4621 ccgcgatgga cggcacgacc gaccggaacg cgctgctcgc cctggcccgc gaactcggcc
     4681 gggtgctcgg ccggctgcac agggtgccgc tgaccgggaa caccgtgctc accccccatt
     4741 ccgaggtctt cccggaactg ctgcgggaac gccgcgcggc gaccgtcgag gaccaccgcg
     4801 ggtggggcta cctctcgccc cggctgctgg accgcctgga ggactggctg ccggacgtgg
     4861 acacgctgct ggccggccgc gaaccccggt tcgtccacgg cgacctgcac gggaccaaca
     4921 tcttcgtgga cctggccgcg accgaggtca ccgggatcgt cgacttcacc gacgtctatg
     4981 cgggagaccc ccgctacagc ctggtgcaac tgcatctcaa cgccttccgg ggcgaccgcg
     5041 agatcctggc cgcgctgctc gacggggcgc agtggaagcg gaccgaggac ttcgcccgcg
     5101 aactgctcgc cttcaccttc ctgcacgact tcgaggtgtt cgaggagacc ccgctggatc
     5161 tctccggctt caccgatccg gaggaactgg cgcagttcct ctgggggccg ccggacaccg
     5221 cccccggcgc ctgacaatcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg
     5281 taaattgtca caacgccgcg gccaattcgc atcgattgtc agaccaagca tgctttcatg
     5341 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     5401 tcaacacgtg ctgatcatga ccaaaatccc ttaacgtgag ttacgcgtcg aagccgaata
     5461 tctagaatta ttccattgag taagttttta agcacatcag cttcaaaagc gctctgaagt
     5521 tcctatactt tctagagaat aggaacttcg gaataggtac ttcaagatcc ccaattcgat
     5581 catgcatgag ctcactagtc ttaagaccca ctttcacatt taagttgttt ttctaatccg
     5641 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
     5701 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
     5761 ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg
     5821 ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
     5881 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
     5941 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc
     6001 cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct
     6061 aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca
     6121 cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc
     6181 tctaatgcgc tgttaatcac tttactttta tctaatctag acatcattaa ttcctaattt
     6241 ttgttgacac tctatcgttg atagagttat tttaccactc cctatcagtg atagagaaaa
     6301 gaattcaaaa gatctaaaga ggagaaagga tctatggata agaaatactc aataggctta
     6361 gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
     6421 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
     6481 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
     6541 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
     6601 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
     6661 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
     6721 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
     6781 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
     6841 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
     6901 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
     6961 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
     7021 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
     7081 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
     7141 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
     7201 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
     7261 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     7321 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     7381 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     7441 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     7501 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     7561 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
     7621 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     7681 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     7741 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     7801 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     7861 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     7921 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     7981 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     8041 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     8101 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     8161 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     8221 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     8281 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     8341 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     8401 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     8461 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     8521 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     8581 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     8641 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
     8701 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     8761 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     8821 attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac
     8881 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     8941 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     9001 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     9061 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     9121 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     9181 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     9241 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
     9301 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     9361 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
     9421 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     9481 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     9541 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     9601 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     9661 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     9721 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     9781 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     9841 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     9901 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     9961 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
    10021 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
    10081 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
    10141 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
    10201 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
    10261 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
    10321 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
    10381 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactaa
    10441 ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
    10501 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
    10561 cgggtgggcc tttctgcgtt tatacctagg gatatattcc gcttcctcgc tcactgactc
    10621 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
    10681 cctggaagat gccaggaaga tacttaacag ggaagtctgc aggaattcct cgagaagctt
    10741 gggcc
//
