LOCUS       sequence-432321-       11871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express 
            HaloTag-MDC1 with a deletion of the BRCT domain in human cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Heyza JR, Mikhova M, Bahl A, Broadbent DG, Schmidt JC
  TITLE     Systematic analysis of the molecular and biophysical properties of 
            key DNA damage response factors.
  JOURNAL   Elife. 2023 Jun 21;12:e87086. doi: 10.7554/eLife.87086.
  PUBMED    37341699
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-21"; volume: "12:e87086. doi"; pages: " 
            10.7554/eLife.87086"
COMMENT     Sequence Label: Halo-MDC1 BRCT domain deletion
FEATURES             Location/Qualifiers
     source          1..11871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1070..1135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1136..1169
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1178..2068
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2081..2101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(7822..7840)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7872..7993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7883..7902)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7937..7956
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8238..8595
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8238..8429
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8261..8281)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8446..8581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8646..9440
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8700..8719)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9310..9329
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9504..9552
                     /note="synthetic polyadenylation signal"
     promoter        9708..9812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9813..9881,9882..10673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10031..10050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10831..11419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10911..10930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11535..11818
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg actacaaaga
     1081 ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg acaagataac
     1141 ttcgtataat gtatgctata cgaagttatc cggtaccatg gcagaaatcg gtactggctt
     1201 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     1261 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     1321 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     1381 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     1441 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     1501 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     1561 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     1621 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1681 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1741 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1801 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1861 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1921 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     1981 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     2041 ctggctgtcg acgctcgaga tttccggcga gccaaccact gaggatctgt actttcagag
     2101 cgataacgcg atcgcttcca tggaggacac ccaggctatt gactgggatg ttgaagaaga
     2161 ggaggagaca gagcaatcca gtgaatcctt gaggtgtaac gtggagccag tagggcggct
     2221 acatatcttt agtggtgccc atggaccaga aaaagatttc ccactacacc tcgggaagaa
     2281 tgtggtaggc cgaatgcctg actgctctgt ggccctgccc tttccatcta tctccaaaca
     2341 acatgcagag attgaaatct tagcctggga caaggcacct atcctccgag actgtgggag
     2401 ccttaatggt actcaaatcc tgagacctcc taaggttttg agccctgggg tgagtcaccg
     2461 tctgagggac caggaattga ttctctttgc tgacttgctc tgccagtacc atcgcctgga
     2521 tgtctctctg ccctttgtct cccggggccc tctgacagta gaagagacac ccagagtaca
     2581 gggagaaact caaccccaga ggcttctgtt ggctgaggac tcggaggagg aagtagattt
     2641 tctttctgaa aggcgtatgg taaaaaaatc aaggaccaca tcttcctctg tgatagttcc
     2701 agagagtgat gaagaggggc attccccggt cctgggcggc cttgggccgc cttttgcctt
     2761 caatttgaac agtgacacag atgtggaaga aggtcagcaa ccagccacag aggaggcctc
     2821 ctcagctgcc agaagaggtg ccactgtaga ggcaaagcag tctgaagctg aagttgtaac
     2881 tgaaatccag cttgaaaagg atcagccttt agtgaaggag agggacaatg atacaaaagt
     2941 caagaggggt gcagggaatg gggtggttcc agctggggtg attctggaga ggagccaacc
     3001 tcctggagag gacagtgaca cagatgtgga tgatgacagc aggcctcctg gaaggccagc
     3061 tgaggtccat ttggaaaggg ctcagccttt tggcttcatc gacagcgaca ctgatgcgga
     3121 agaagagagg atcccagcaa ccccagttgt cattcctatg aagaagagga agatcttcca
     3181 tggagtaggt acaaggggtc ctggagcacc aggcctggcc catctgcagg agagccaggc
     3241 tggtagtgat acagatgtgg aagaaggcaa ggccccacag gctgtccctc tggagaaaag
     3301 ccaagcttcc atggttatca acagcgatac agatgacgag gaagaagtct cagcagcgct
     3361 gactttggca catctgaaag agagccagcc tgctatatgg aacagagatg cagaagagga
     3421 catgccccaa cgtgtggtcc ttctgcagcg aagccaaacc accactgaga gagacagtga
     3481 cacagacgtg gaggaggaag agctcccagt ggaaaataga gaagctgtcc tcaaggatca
     3541 cacaaagatt agagcccttg ttagagcaca ttcagaaaag gaccaacctc cttttgggga
     3601 cagtgatgac agtgtggaag cagataagag ctcacctggg atccacctgg agagaagcca
     3661 agcctccacc acagtggaca tcaacacaca agtggagaag gaagtcccgc cagggtcagc
     3721 cattatgcat ataaagaagc atcaggtgtc tgtggagggg acaaatcaaa cagatgtgaa
     3781 agcagttggg ggaccagcaa agctgcttgt ggtatctcta gaggaagcct ggcctctgca
     3841 tggggactgt gaaacagatg cagaggaggg cacctcccta acagcctcag tagttgcaga
     3901 tgtaagaaag agccagcttc cagcagaagg ggatgctggg gcagagtggg ctgcagctgt
     3961 tcttaagcag gagagagctc atgaggtggg ggcccagggt gggccacctg tggcacaagt
     4021 ggagcaggac ctccctatct caagagagaa cctcacagat ctggtggtgg acacagacac
     4081 tctaggggaa tccacccagc cacagagaga gggagcccag gtccccacag gaagggagag
     4141 agaacaacat gtgggtggga ccaaggactc tgaagacaac tatggtgatt ctgaagatct
     4201 ggacctacaa gctacccagt gctttctgga gaatcagggc ctggaagcag tccagagcat
     4261 ggaggatgaa cctacccagg ccttcatgtt gactccaccc caagagcttg gcccttccca
     4321 ttgcagcttc cagacaacag gtaccctaga tgaaccatgg gaggtcctgg ctacacagcc
     4381 attctgtctg agagagtctg aggactctga gacccagcct tttgacacgc accttgaggc
     4441 ctatggacct tgcctgtctc cacctagggc aataccagga gaccaacatc cagagagccc
     4501 agttcacaca gagccaatgg ggattcaagg cagagggagg cagactgtgg ataaagtcat
     4561 gggtatacca aaagaaacag cagagagggt gggccctgag agagggccat tggagagaga
     4621 aactgagaaa ctgctaccag aaagacagac agatgtgaca ggagaggaag aattaaccaa
     4681 ggggaaacag gacagagaac aaaaacagtt gttagctaga gacacccaga gacaagaatc
     4741 tgacaaaaat ggggaaagtg caagtcctga aagagatagg gagagtttga aggtagaaat
     4801 tgagacatct gaggaaatac aagagaaaca agtacagaag cagacccttc caagcaaagc
     4861 atttgagaga gaagtagaga gaccagtagc aaacagagag tgcgatccag ccgagttaga
     4921 agagaaggtg cccaaagtga tcctggagag agatacacag agaggggagc cagagggagg
     4981 gagccaggac cagaaagggc aggcctccag cccaacacca gagcctgggg tgggggcggg
     5041 ggaccttccg ggacctacct cagcccccgt accttctggg agccagtcag gtggaagggg
     5101 atccccagtg agccccagga ggcatcagaa aggcctcctg aattgcaaga tgccacctgc
     5161 tgagaaggct tccaggatca gagctgctga gaaggtttcc aggggcgatc aggaatctcc
     5221 agatgcttgt ctgcctcctg cagtacctga agccccagcc ccaccccaaa agccccttaa
     5281 ctctcagagc cagaaacatc ttgcacctcc gccccttctt tctccccttt taccttctat
     5341 caagccaacc gttcgtaaga ccaggcaaga tgggagtcag gaagctccag aggctccctt
     5401 gtcctcagag ctggagcctt tccacccaaa gcctaaaatt agaactcgga agtcctccag
     5461 aatgacaccc tttccagcta cctctgctgc ccctgagccc cacccttcca cctccacagc
     5521 ccagccagtc actcccaagc ccacatctca ggccactagg agcaggacaa ataggtcctc
     5581 tgtcaagacc cctgaaccag ttgtccccac agcccctgag ctccagcctt ccacctccac
     5641 agaccagcct gtcacctctg agcccacatc tcaggttact aggggaagaa aaagtagatc
     5701 ctctgtcaag acccctgaaa cagttgtgcc cacagccctt gagctccagc cttccacctc
     5761 caccgaccga cctgtcacct ctgaacccac ctctcaggct actaggggaa gaaaaaatag
     5821 atcctctgtc aagacccctg aaccagttgt ccccacagcc cctgagctcc agccttccac
     5881 ctccacagac cagcctgtca cttctgagcc cacatatcag gctactaggg gaagaaaaaa
     5941 tagatcctct gtcaagaccc ctgaaccagt tgtgcccaca gcccctgagc tccggccttc
     6001 cacctccaca gaccgacctg tcacccccaa gcccacatct cggaccacta ggagcaggac
     6061 aaatatgtcc tctgtcaaga cccctgaaac agttgtcccc acagcccctg agctccagat
     6121 ttccacctcc acagaccaac ctgtcacccc taagcccaca tctcggacca ctaggagcag
     6181 gacaaatatg tcctctgtga agaaccctga atcaactgtc cctatagccc ctgagctccc
     6241 accttccacc tccacagagc agcctgtcac ccctgagccc acatctcggg ctactagggg
     6301 aagaaaaaat agatcctctg gcaagacccc tgaaacactt gtccccacag cccctaagct
     6361 cgagccttcc acttccacag accaacctgt cactcctgag cccacatctc aggccaccag
     6421 gggcaggaca aataggtcct ctgtgaagac ccctgaaaca gttgtcccca cagcccctga
     6481 gctccagcct tccacctcca cagaccagcc tgttacccct gagcctacgt ctcaggctac
     6541 taggggaaga acagatagat cctctgtcaa gactcctgaa acagttgtcc ccacagcccc
     6601 tgagctacag gcttccgcct ccacagacca gcctgtcacc tctgagccca catctcggac
     6661 cactagggga agaaaaaatc ggtcctctgt caagacccct gaaacagttg tgcccgcagc
     6721 ccctgagctc cagcctccca cctccacaga ccgacctgtc acccctgagc ccacatctcg
     6781 ggccactagg ggcaggacaa ataggtcctc tgtcaagacc cctgaatcaa ttgtccctat
     6841 agcccctgag cttcagcctt ccacctccag aaaccagctt gtcacccctg agcccacatc
     6901 tcgggccact aggtgcagga caaataggtc ctctgtcaag acccctgagc cagttgtccc
     6961 cacagcccct gagccccatc ctaccacctc cacagaccag cctgtcaccc ccaagctcac
     7021 atctagggcc actaggagaa agacaaatag gtcctctgtc aagactccca aaccagttga
     7081 accagcagcc tctgatcttg agccttttac ccccacagac cagtccgtca cccctgaggc
     7141 catagctcag ggtggtcaga gcaaaacact gaggtcttcc acagtaagag ctatgccggt
     7201 tcctaccacc cctgaattcc aatctcctgt caccacagac cagcctattt cccctgagcc
     7261 tattactcaa cccagttgca tcaagaggca gagagccgct gggaaccctg gctccctcgc
     7321 agctcccatt gaccataagc cttgctctgc acccttggaa cctaaatccc aggcctcaag
     7381 gaaccaaaga tggggagcag tgagagcagc tgaatccctt acagccattc ctgagcctgc
     7441 ctctccccag cttcttgaga caccaattca tgcctcccag atccaaaagg tggaaccagc
     7501 aggtagatct aggttcaccc cggagctcca gcctaaggcc tctcaaagcc gcaagaggtc
     7561 tttagctacc atggattcac caccacatca aaaacagccc caaagagggg aagtctccca
     7621 gaagacagtg attatcaagg aagaggaaga agatactgca gagaagccag ggaaggaaga
     7681 ggatgtcgtg actccaaaac caggcaagag aaagagagac caggcagagg aggagcccaa
     7741 cagaatacca agccgcagcc tccgacggac caaacttaac caagaatcat gagcggccgc
     7801 ttgctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggccgcttcg
     7861 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7921 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7981 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     8041 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca
     8101 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8161 ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
     8221 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     8281 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8341 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8401 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8581 ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt aaggctagag
     8641 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     8701 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     8761 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     8821 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     8881 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     8941 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     9001 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     9061 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     9121 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     9181 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     9241 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     9301 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     9361 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     9421 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     9481 caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     9541 gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt tcccttgtcc
     9601 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     9661 acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10021 tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10201 acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta accgcttttt
    10261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    10321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    10381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    10441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    10501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    10561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    10621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taattcgaaa
    10681 tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa tacggttatc
    10741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    11281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11401 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11521 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
    11581 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
    11641 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
    11701 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
    11761 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
    11821 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
