LOCUS       sequence-432325-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-MYBBP1A
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             759..782
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             786..809
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             810..836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    852..876
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4873..4897)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4950..4991
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(5048..5072)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5125..5166
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5246..5263)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5252..5476
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5609..5628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5760..5807
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5815..6837
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5815..6837
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6967..7088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7004..7023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7058..7077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7137..7153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7137..7153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7150..7172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7161..7177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7185..7191,7192..7209,7210..7215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7230..7251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7368..7385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7539..8127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7619..7638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8298..9089,9090..9158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9159..9263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9338..9357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccatgca ccaccaccac caccaccacc
      781 acatggacta caaggacgac gatgacaagt acccttatga cgtgcccgat tacgctttga
      841 accagatatc aacaagtttg tacaaaaaag caggctccac catggagagc cgggatcccg
      901 cccagccgat gtcgcctgga gaagcgacgc agagtggcgc ccggcctgcc gaccgctatg
      961 gcctattgaa gcacagtcgc gagttcttgg acttcttctg ggacattgcg aagcctgagc
     1021 aggagacgcg acttgcggcc acggagaagc tgctggagta tctgcgtggc aggccgaagg
     1081 ggtccgagat gaaatatgcc ctgaagcgtc taatcacggg actcggggtc gggcgagaaa
     1141 cagcccggcc ctgctacagt ttggccctgg cacagctgtt acagtctttt gaagacctcc
     1201 ccttgtgcag catcctgcag cagatacaag aaaaatatga cctgcatcag gtgaagaagg
     1261 caatgctgag acctgctctc tttgcaaacc tgtttggagt gctcgccctc tttcagtcag
     1321 gtcggctggt gaaggaccag gaggcactga tgaagtcggt gaagctgctg caggccctgg
     1381 cccagtacca aaaccacttg caggagcagc cccggaaggc cctggtggac atcctctccg
     1441 aggtctcgaa ggccacattg caggagatcc tgccggaggt cctcaaagcc gacttgaata
     1501 taatactcag ctcccctgaa cagctagagc tcttcctcct ggcccagcag aaggtgccct
     1561 ccaagctcaa gaagctggtg ggatccgtga acctattctc agatgagaat gtccccaggc
     1621 tggtgaatgt gctgaagatg gccgcctcct ctgtgaagaa ggaccgcaag ctgcccgcca
     1681 ttgctctgga cctgctccgc ctggcgctca aggaagacaa gttcccacgg ttctggaagg
     1741 aggtggtgga acaagggctg ctgaagatgc agttctggcc agccagctac ctgtgtttcc
     1801 gcctgctggg cgcggccctg cccctgctga ccaaggagca gctgcacctg gtgatgcagg
     1861 gagacgtgat ccgccattac ggggagcacg tgtgcactgc taagctccca aagcagttca
     1921 agtttgcccc agagatggac gattacgtgg gcaccttcct agaggggtgc caggatgacc
     1981 ctgagcggca gctggccgtg ctagtggcct tctcatctgt caccaaccaa ggcctccctg
     2041 tcacgcctac tttctggcgg gtcgtgcggt tcctgagccc tccggccctg cagggctatg
     2101 tggcctggct gcgggccatg tttctccagc cagacctgga ctccttggtt gacttcagca
     2161 ccaacaacca gaagaaagcc caggattcat cgctccacat gcctgagcga gctgtgttcc
     2221 ggctgaggaa atggatcatc tttcgattgg tgagcattgt ggacagcctg cacctggaga
     2281 tggaggaggc cttgactgag caggtggcca ggttttgttt gttccactcg ttctttgtca
     2341 caaagaagcc cacatcccag atccctgaga caaagcaccc gttctccttc cctttggaaa
     2401 accaggcccg agaggctgtc agcagtgcct tcttcagtct gttgcagacc ctcagcacgc
     2461 agttcaagca ggcaccgggc cagacccagg gtgggcagcc ctggacctac cacctggtgc
     2521 agttcgcaga cctcctgttg aatcacagcc acaacgtgac caccgtgaca cccttcactg
     2581 cgcagcagcg ccaggcctgg gaccggatgc tgcagactct gaaggagctg gaggcccact
     2641 ccgcagaggc cagggctgct gccttccagc accttctgct cctcgtgggc atccacctcc
     2701 tcaagtcccc tgcagagagc tgtgacctgc tgggtgacat ccagacctgc atcaggaaaa
     2761 gtctgggaga gaagccccgc cggagccgca ccaagaccat cgacccccag gaacccccgt
     2821 gggtagaggt gctggtggag atcttgctgg ccctgttggc ccagcccagc cacctcatgc
     2881 gccaggtggc ccggagcgtg tttggccaca tctgctccca cctgaccccg cgtgccctgc
     2941 agctaattct ggatgtgctg aaccccgaga ccagtgagga tgagaatgac cgtgtggtgg
     3001 tgacggacga ttctgatgag cggcggctga agggtgcaga ggacaagagc gaggaaggtg
     3061 aggacaacag aagctcagag agtgaagagg agagcgaggg ggaggagagc gaggaggagg
     3121 agcgcgacgg ggacgtggat cagggcttcc gggaacagct gatgaccgtg ctgcaggctg
     3181 ggaaggcgct gggtggagag gacagtgaga acgaggagga gctgggggat gaggccatga
     3241 tggccctgga ccagagcctc gccagcctct ttgccgagca gaagctgcgt atccaggccc
     3301 ggcgagacga gaagaacaag ctgcagaagg agaaggctct gcggcgcgac ttccagatcc
     3361 gggtgctgga cctggtggag gtgctagtga ccaagcagcc cgagaatgcc ctggtcctgg
     3421 agctgctgga gccgctgctg agcatcatcc ggcgcagcct gcgcagcagc agctccaaac
     3481 aggagcagga ccttctgcac aagacggcgc gcatcttcac gcaccacctg tgccgtgccc
     3541 ggcgctactg ccacgacttg ggtgagcgcg caggggccct gcacgcccag gtggagcggt
     3601 tggtgcagca ggctggccgc cagcccgact cccccaccgc cctctaccac ttcaacgcct
     3661 ctctctacct gctccgggtc ttgaagggca acactgctga gggctgcgtg catgagacac
     3721 aggagaagca gaaagctggc actgacccca gccacatgcc cacgggcccg caggctgcca
     3781 gctgcttgga cttgaacctg gtgacccggg tgtactcgac agcactgagc tccttcctga
     3841 ccaagcgcaa cagccccctc acagttccca tgttcctcag cctcttctcc cggcacccgg
     3901 tgctctgtca gagcctgctc cccatcctgg tccagcatat cacgggcccg gtgcggcccc
     3961 gtcgtcaggc ctgcctgctg ctccagaaga ccctgtccat gcgggaggtg aggtcgtgct
     4021 ttgaggaccc cgagtggaag cagctgatgg gccaggtcct agcaaaggtc accgagaact
     4081 tgcgcgtgct gggggaggcg cagaccaagg cgcagcatca gcaggcactg tcctccctgg
     4141 agctgctcaa cgttctcttc aggacctgca aacatgagaa gctgaccttg gacctgacgg
     4201 tgctcctggg tgtgctgcag gggcaacagc agagcctaca gcagggggca cactccaccg
     4261 gctccagccg cctgcacgac ctctactggc aggccatgaa aaccctggga gtccagcgcc
     4321 ccaagttgga gaagaaggat gccaaggaga tccccagtgc cacccagagc cccatcagta
     4381 agaagcggaa gaaaaaggga ttcttgccag agacgaagaa gcgcaagaaa cgcaagtcag
     4441 aggatggcac gccagcggag gatggcacac ctgcagccac cggcgggagc cagcccccca
     4501 gcatgggcag gaagaagagg aacaggacaa aggctaaggt cccagcccag gcaaacggga
     4561 cgccaaccac caagagtcca gcccctggcg cccccacccg gagccccagc acccctgcca
     4621 aatccccaaa actgcagaag aaaaaccaga agccgtccca ggtgaatgga gctcccgggt
     4681 cccccacgga acctgcaggc caaaagcagc atcagaaggc tcttcccaaa aagggggtct
     4741 tgggcaaatc accactgtcc gcgctggcac ggaaaaaggc aaggctgtct ttggtcatca
     4801 ggagtcccag cctgcttcag agtggggcca agaagaaagc acaggtgagg aaggcaggga
     4861 agccctgaat ccacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     4921 gctcaagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     4981 tcgattctac gcgtacctga tccgcggccg catagataac tgatccagtg tgcctcgaga
     5041 tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctca
     5101 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     5161 tctacgcgta cctgatccgc ggccgcatag ataactgatc cagtgtgcct cgagtctaga
     5221 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5281 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5341 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5401 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5461 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5521 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5581 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5641 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5701 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     5761 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     5821 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     5881 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     5941 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6001 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6061 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6121 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     6181 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     6241 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     6301 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     6361 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     6421 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     6481 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     6541 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     6601 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     6661 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     6721 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     6781 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     6841 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6901 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6961 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7081 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9301 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     9361 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     9421 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     9481 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
