LOCUS       sequence-432341-       10679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human G9a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Wang L, Xu S, Lee JE, Baldridge A, Grullon S, Peng W, Ge K
  TITLE     Histone H3K9 methyltransferase G9a represses PPARgamma expression 
            and adipogenesis.
  JOURNAL   EMBO J. 2012 Nov 23. doi: 10.1038/emboj.2012.306.
  PUBMED    23178591
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2012 Nov 23. doi: 10.1038/emboj.2012.306."
COMMENT     Sequence Label: MSCVhygro-F-G9a
FEATURES             Location/Qualifiers
     source          1..10679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1420..1429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1429..1452
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        5174..5673
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5209..5232)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5606..5625
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5691..6716
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5691..6716
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7074..7588
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7750..7766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7774..7780,7781..7798,7799..7804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7819..7840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7957..7974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8128..8716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8208..8227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8887..9678,9679..9747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9510..9529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9748..9852)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9920..9938
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9976..9998)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10098..10117
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10311..10333
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10461..10480
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatcctgg ccaccatgga ctacaaggac
     1441 gacgatgaca agatggcggc ggcggcggga gctgcagcgg cggcggccgc cgagggggag
     1501 gcccccgctg agatgggggc gctgctgctg gagaaggaaa ccagaggagc caccgagaga
     1561 gttcatggct ctttggggga cacccctcgt agtgaagaaa ccctgcccaa ggccaacccc
     1621 gactccctgg agcctgctgg cccctcatct ccagcctctg tcactgtcac tgttggtgat
     1681 gagggggctg acacccctgt aggggctaca ccactcattg gggatgaatc tgagaatctt
     1741 gagggagatg gggacctccg tgggggccgc atcctgctgg gccatgccac aaagtcattc
     1801 ccctcttccc ccagcaaggg gggttcctgt cctagccggg ccaagatgtc aatgacaggg
     1861 gcgggaaaat cacctccatc tgtccagagt ttggctatga ggctactgag tatgccagga
     1921 gcccagggag ctgcagcagc agggtctgaa ccccctccag ccaccacgag cccagaggga
     1981 cagcccaagg tccaccgagc ccgcaaaacc atgtccaaac caggaaatgg acagcccccg
     2041 gtccctgaga agcggccccc tgaaatacag catttccgca tgagtgatga tgtccactca
     2101 ctgggaaagg tgacctcaga tctggccaaa aggaggaagc tgaactcagg aggtggcctg
     2161 tcggaggagt taggttctgc ccggcgttca ggagaagtga ccctgacgaa aggggacccc
     2221 gggtccctgg aggagtggga gacggtggtg ggtgatgact tcagtctcta ctatgattcc
     2281 tactctgtgg atgagcgcgt ggactccgac agcaagtctg aagttgaagc tctaactgaa
     2341 caactaagtg aagaggagga ggaggaagag gaggaagaag aagaagagga agaggaggag
     2401 gaagaggaag aagaagagga agatgaggag tcagggaatc agtcagatag gagtggttcc
     2461 agtggccggc gcaaggccaa gaagaaatgg cgaaaagaca gcccatgggt gaagccgtct
     2521 cggaaacggc gcaagcggga gcctccgcgg gccaaggagc cacgaggagt gaatggtgtg
     2581 ggctcctcag gccccagtga gtacatggag gtccctctgg ggtccctgga gctgcccagc
     2641 gaggggaccc tctcccccaa ccacgctggg gtgtccaatg acacatcttc gctggagaca
     2701 gagcgagggt ttgaggagtt gcccctgtgc agctgccgca tggaggcacc caagattgac
     2761 cgcatcagcg agagggcggg gcacaagtgc atggccactg agagtgtgga cggagagctg
     2821 tcaggctgca atgccgccat cctcaagcgg gagaccatga ggccatccag ccgtgtggcc
     2881 ctgatggtgc tctgtgagac ccaccgcgcc cgcatggtca aacaccactg ctgcccgggc
     2941 tgcggctact tctgcacggc gggcaccttc ctggagtgcc accctgactt ccgtgtggcc
     3001 caccgcttcc acaaggcctg tgtgtctcag ctgaatggga tggtcttctg tccccactgt
     3061 ggggaggatg cttctgaagc tcaagaggtg accatccccc ggggtgacgg ggtgacccca
     3121 ccggccggca ctgcagctcc tgcaccccca cccctgtccc aggatgtccc cgggagagca
     3181 gacacttctc agcccagtgc ccggatgcga gggcatgggg aaccccggcg cccgccctgc
     3241 gatcccctgg ctgacaccat tgacagctca gggccctccc tgaccctgcc caatgggggc
     3301 tgcctttcag ccgtggggct gccactgggg ccaggccggg aggccctgga aaaggccctg
     3361 gtcatccagg agtcagagag gcggaagaag ctccgtttcc accctcggca gttgtacctg
     3421 tccgtgaagc agggcgagct gcagaaggtg atcctgatgc tgttggacaa cctggacccc
     3481 aaattccaga gcgaccagca gagcaagcgc acgcccctgc atgcagccgc ccagaagggc
     3541 tccgtggaga tctgccatgt gctgctgcag gctggagcca acataaatgc agtggacaaa
     3601 cagcagcgga cgccactgat ggaggccgtg gtgaacaacc acctggaggt agcccgttac
     3661 atggtgcagc gtggtggctg tgtctatagc aaggaggagg acggttccac ctgcctccac
     3721 cacgcagcca aaatcgggaa cttggagatg gtcagcctgc tgctgagcac aggacaggtg
     3781 gacgtcaacg cccaggacag tggggggtgg acgcccatca tctgggctgc agagcacaag
     3841 cacatcgagg tgatccgcat gctactgacg cggggcgccg acgtcaccct cactgacaac
     3901 gaggagaaca tctgcctgca ctgggcctcc ttcacgggca gcgccgccat cgccgaagtc
     3961 cttctgaatg cgcgctgtga cctccatgct gtcaactacc atggggacac ccccctgcac
     4021 atcgcagctc gggagagcta ccatgactgc gtgctgttat tcctgtcacg tggggccaac
     4081 cctgagctgc ggaacaaaga gggggacaca gcatgggacc tgactcccga gcgctccgac
     4141 gtgtggtttg cgcttcaact caaccgcaag ctccgacttg gggtgggaaa tcgggccatc
     4201 cgcacagaga agatcatctg ccgggacgtg gctcggggct atgagaacgt gcccattccc
     4261 tgtgtcaacg gtgtggatgg ggagccctgc cctgaggatt acaagtacat ctcagagaac
     4321 tgcgagacgt ccaccatgaa catcgatcgc aacatcaccc acctgcagca ctgcacgtgt
     4381 gtggacgact gctctagctc caactgcctg tgcggccagc tcagcatccg gtgctggtat
     4441 gacaaggatg ggcgattgct ccaggaattt aacaagattg agcctccgct gattttcgag
     4501 tgtaaccagg cgtgctcatg ctggagaaac tgcaagaacc gggtcgtaca gagtggcatc
     4561 aaggtgcggc tacagctcta ccgaacagcc aagatgggct ggggggtccg cgccctgcag
     4621 accatcccac aggggacctt catctgcgag tatgtcgggg agctgatctc tgatgctgag
     4681 gctgatgtga gagaggatga ttcttacctc ttcgacttag acaacaagga tggagaggtg
     4741 tactgcatag atgcccgtta ctatggcaac atcagccgct tcatcaacca cctgtgtgac
     4801 cccaacatca ttcccgtccg ggtcttcatg ctgcaccaag acctgcgatt tccacgcatc
     4861 gccttcttca gttcccgaga catccggact ggggaggagc tagggtttga ctatggcgac
     4921 cgcttctggg acatcaaaag caaatatttc acctgccaat gtggctctga gaagtgcaag
     4981 cactcagccg aagccattgc cctggagcag agccgtctgg cccgcctgga cccacaccct
     5041 gagctgctgc ccgagctcgg ctccctgccc cctgtcaaca catgagaacg gaccacaccc
     5101 tctctcccca gcatggatgg ccacagctca gccgcctcct ctgccacctg actcgaggtt
     5161 aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5221 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5281 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5341 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     5401 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     5461 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     5521 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     5581 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     5641 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc
     5701 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     5761 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     5821 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     5881 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     5941 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     6001 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     6061 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     6121 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     6181 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     6241 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     6301 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     6361 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     6421 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     6481 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     6541 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     6601 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     6661 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     6721 gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct
     6781 agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc
     6841 gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga
     6901 ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc
     6961 cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatccgtc gacctgcagc
     7021 caagcttatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     7081 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     7141 tacataactg agaatagaga agttcagatc aaggttagga acagagagac agcagaatat
     7201 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     7261 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
     7321 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     7381 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     7441 cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc aataaaccct
     7501 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     7561 tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg gagaacaaca
     7621 ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact gttttgaatc
     7681 cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga gctggggaga
     7741 attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9181 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9241 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9301 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9361 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9421 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9481 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9541 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9601 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9661 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9721 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9781 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9841 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     9901 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     9961 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10021 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10081 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10141 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10201 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10261 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10321 acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
    10381 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
    10441 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
    10501 gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca atttgttaaa
    10561 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
    10621 atagagtacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggg
//
