LOCUS       sequence-432343-        6355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated rat TH promoter expressing iCre fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Lycas MD, Ejdrup AL, Sorensen AT, Haahr NO, Jorgensen SH, Guthrie 
            DA, Stoier JF, Werner C, Newman AH, Sauer M, Herborg F, Gether U
  TITLE     Nanoscopic dopamine transporter distribution and conformation are 
            inversely regulated by excitatory drive and D2 autoreceptor 
            activity.
  JOURNAL   Cell Rep. 2022 Sep 27;40(13):111431. doi: 
            10.1016/j.celrep.2022.111431.
  PUBMED    36170827
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-09-27"; volume: "40(13):111431. doi"; pages: " 
            10.1016/j.celrep.2022.111431"
COMMENT     Sequence Label: pAAV-pTH-iCre:EGFP-WPREpA
FEATURES             Location/Qualifiers
     source          1..6355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      324..912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     813..832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   974..1103
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             1639..1659
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2692..2694,2695..3408)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2692..2694,2695..3405)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2734..2755)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2995..3014)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3342..3363
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3455..4043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3508..3528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3926..3937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4075..4551
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4274..4293)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4591..4731
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4591..4720
                     /label=AAV2 ITR
     rep_origin      4806..5261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4893..4912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5103..5124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5278..5297)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5397..5419
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5457..5475)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5543..5647
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5648..5716,5717..6355,1..153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5866..5885)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
       61 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      121 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      181 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      241 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      961 tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg
     1021 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     1081 caactccatc actaggggtt cctgcggccg cacgcgtata gatagtacac tttgttacat
     1141 gggctggggg gaacatggcc catgtcctgg aggggacttt atgacagaca tccaaaaatc
     1201 cagtgagagg gcttctagat ttgtctccaa aggttatagt tctaacatga gcccttagga
     1261 aatccagcat ggttctccct gtgtgccctg gtttggttag agagctctag cggtctcctg
     1321 tcccacagaa taccagccag cccctgccct acgtcgtgcc tcgggctgag ggtgattcag
     1381 aggcaggtgc ctgtgacagt ggatgcaatt agatctaatg ggacggaggc ctttctcgtc
     1441 gccctcgctc catgcccacc cccgcctccc tcaggcacag caggcgtgga gaggatgcgc
     1501 aggaggtagg aggtggggga cccagagggg ctttgacgtc agcctggcct ttaaagaggg
     1561 cgcctgcctg gcgagggctg tggagacaga actcgggacc accagggatc cttcgaaacc
     1621 ggtgctagca ccatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac
     1681 caaaacctgc ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg
     1741 gacatgttca gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg
     1801 tgcagatcct gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct
     1861 gaggatgtga gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc
     1921 caacagcacc tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct
     1981 gactccaatg ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg
     2041 gagagagcca agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg
     2101 atggagaact ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac
     2161 aacaccctgc tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc
     2221 gatggtggga gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt
     2281 gtggagaagg ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct
     2341 ggtgtggctg atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct
     2401 gccccttctg ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc
     2461 caccgcctga tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc
     2521 cactctgcca gagtgggtgc tgccagggac atggccaggg ctggtgtgtc catccctgaa
     2581 atcatgcagg ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg
     2641 gactctgaga ctggggccat ggtgaggctg ctcgaggatg gggacgttaa cgtgagcaag
     2701 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2761 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2821 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2881 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2941 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3001 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3061 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3121 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3181 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3241 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3301 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3361 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagt cgacggcgcg
     3421 cccctgcagg gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3481 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3541 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3601 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3661 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3721 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3781 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3841 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     3901 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3961 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4021 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     4081 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4141 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4201 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4261 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4321 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     4381 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     4441 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4501 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     4561 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4621 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4681 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4741 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4801 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4861 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4921 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4981 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5041 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5101 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     5161 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5221 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5281 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5341 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5401 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5461 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5521 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5581 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5641 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5701 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5761 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5821 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5881 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5941 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6001 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6061 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6121 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6181 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6241 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6301 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggt
//
