LOCUS       sequence-432344-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-229E NSP15_stop
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1151..1250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1267..1286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1738..2529
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2622..3210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3111..3130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3364..3381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3540..3567)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3659..3745)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3794..3816
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3808..3825
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3809..3825
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgggtttaga gaacattgct
      121 tttaacgttg ttaataaagg ttcatttgtt ggtgctgatg gtgaattacc agtagccatt
      181 agtggtgata aagtgttcgt acgtgatggt aacactgata atttagtctt tgttaacaaa
      241 acatcactgc ctacaaacat agcatttgaa ctttttgcta agaggaaggt tggtttaaca
      301 ccacctctca gtattctcaa aaaccttggt gttgtcgcca catataagtt tgtcttgtgg
      361 gattatgaag ctgagcgtcc cttgacaagc tttactaagt ctgtttgtgg ttatacagac
      421 tttgcagagg atgtttgtac ttgttacgat aatagtatac aaggttcata cgaacgtttt
      481 actctgtcaa ctaatgctgt gttattctct gctactgctg tgaaaacagg tggtaagagt
      541 ttgccggcta ttaaattgaa ttttggaatg cttaatggta atgcaattgc tactgtcaaa
      601 tcagaagatg gtaacataaa aaatattaac tggtttgttt acgtacgcaa agatggcaaa
      661 cctgttgatc attatgatgg tttttatacc caaggtcgta atttacaaga ctttttgcct
      721 cgcagcacaa tggaagaaga ctttttgaac atggatatag gcgtgtttat tcaaaagtat
      781 ggtctagagg atttcaactt cgagcacgtt gtgtatggtg atgtttcaaa aactactcta
      841 ggcggtttac acttgttgat ttcacaagta cgtctgagta aaatgggcat cttaaaggca
      901 gaggagtttg tggcagcatc tgacataaca ctcaaatgtt gtactgtgac ttatcttaat
      961 gatcctagtt ctaagactgt ttgtacttac atggatttgt tgttggatga ttttgtttct
     1021 gtattgaagt ctttggattt gactgttgta tccaaggttc atgaggtcat aattgacaac
     1081 aaaccatgga gatggatgct atggtgtaaa gataatgccg ttgctacatt ctatcctcag
     1141 ttgcagtaat acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1201 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     1381 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     1441 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1501 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1561 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1621 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1681 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     1741 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1801 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1861 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1921 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     1981 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2041 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2101 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2161 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2221 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2281 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2341 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2401 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2461 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2521 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     2581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3421 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3481 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3541 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3601 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3661 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3721 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3781 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
