LOCUS       sequence-432383-       11109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse integrin a5 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Yuxin Hao, Jiabin Yan, Courtney Fraser, Aiping Jiang, Murali 
            Anuganti, Roushu Zhang, Kenneth Lloyd, Joseph Jardine, Jessica 
            Coppola, Rob Meijers, Jing Li, Timothy A.
  TITLE     Synthetic integrin antibodies discovered by yeast display reveal 
            alpha-V subunit pairing preferences with beta subunits
  JOURNAL   bioRxiv 2024.01.26.577394
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.26.577394"
COMMENT     Sequence Label: mouse a5 full length
FEATURES             Location/Qualifiers
     source          1..11109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3063..3119
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    5433..5488
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5487..5506)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6198..6786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6278..6297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6872..7681)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7595..7614
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7700..7747)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(8173..8772)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8256..8276)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8753..8772
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8782..9281)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8830..8849)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9223..9246
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        9365..9668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9670..9945
                     /label=chicken beta-actin promoter
     intron          9946..10962
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10886..10908
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10970..10989
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ctggcgccct cgcgttcaac ctagacgcgg aggccccggc ggtgctctcc gggccccccg
       61 gctccctttt tggcttctcc gtggagtttt accggccggg aagggacgga gtcagtgtgc
      121 tggttggggc acccaaggct aacactagcc agccaggtgt actgcaaggt ggtgctgtct
      181 atgtgtgtcc ctggggcacc agtcctatcc agtgcaccac cattcaattt gacagcaaag
      241 gctcccggat tctggagtcc tcactgtaca gtgccaaggg agaggagcct gtggagtaca
      301 agtccttgca gtggttcgga gcaacagttc gggcccatgg ctcctccatc ttggcatgtg
      361 ctccactgta tagctggcgc acagaaaagg acccacagaa tgacccagtg ggcacctgct
      421 acctctccac agaaaacttc acccggattc tggagtacgc accttgccgc tcagattttg
      481 gcagtgcagc agggcagggc tactgccaag ggggcttcag tgctgagttc accaagactg
      541 gccgtgtggt cctgggtgga cctggaagct acttctggca aggccagatc ctgtccgcca
      601 ctcaagagca gatctcggag tcctattacc cagagtatct catcaaccct gttcaggggc
      661 agctgcagac ccgccaggcc agctccgtct atgatgacag ctacttggga tactctgtgg
      721 ctgtgggtga attcagtggt gatgacacag aagactttgt tgctggcgtg cccaagggga
      781 acctcaccta tggctatgtc accgtcctta atggctcaga catccactcc ctctacaacg
      841 tctcaggaga acagatggcc tcctacttcg gctatgctgt ggctgccact gataccaatg
      901 gagatgggct agatgaccta ctggtagggg cacccctgct catggagcgg acagctgatg
      961 ggagacctca ggaggtgggc agggtctaca tctatctgca gcgcccagcg ggcatagatc
     1021 ccacacccac cctcaccctc actgggcaag atgagttcag ccgattcggc agctccttga
     1081 cacccttggg ggacctggac caagacggct acaatgatgt cgccattggg gctccatttg
     1141 gtggggaggc ccagcaggga gtcgtattta tattcccggg aggcccagga ggactgagca
     1201 ctaaaccttc ccaggttttg cagcctcttt gggcagctgg ccgtacccca gacttctttg
     1261 gctctgccct tcgaggagga cgagatctgg atggcaatgg ataccctgat ctaatcgttg
     1321 gatcctttgg tgtggacaag gctctggtgt acagagggcg gcccatcata tctgccagcg
     1381 catctctcac catcttcccc tccatgttca acccagagga gcgcagttgc agcctggaag
     1441 ggaaccctgt gtcctgcatc aaccttagct tctgcctcaa tgcctctgga aaacatgtcc
     1501 ccaactctat aggcttcgag gtggaactcc aactggactg gcagaagcaa aagggagggg
     1561 tccggcgggc actgttcctg acttccaagc aggccaccct tacccagacc ctgcttatcc
     1621 agaatggggc tcgggaggac tgcagggaga tgaagatcta cctcaggaat gaatcagaat
     1681 tcagagacaa actctcccca attcacattg ccctcaactt ctccttggac cccaaagctc
     1741 ccatggacag ccatggcctc cggccagttc tacactacca aagcaaaagc aggatagagg
     1801 acaaggccca gatcttgctg gactgtggtg aagacaatat ctgtgtgcct gacctgcagc
     1861 tggatgtgta tggggagaag aaacatgtgt acctgggtga caagaacgca ctgaacctga
     1921 cattccatgc ccaaaatctg ggtgagggcg gtgcctatga agccgagctt cgggtcacag
     1981 cccctctaga ggccgagtac tcaggacttg tcagacaccc agggaacttc tccagcctga
     2041 gctgtgacta ctttgctgtg aaccagagcc gccagctggt gtgtgacctg ggcaacccca
     2101 tgaaggcagg caccagtctc tggggtggcc ttcggttcac tgttcctcat cttcaagaca
     2161 caaagaaaac catccagttt gactttcaga tcctcagcaa gaacctgaac aactcacaaa
     2221 gcaacgtggt ctccttccca ctctcggtgg aggctcaagc ccaggtctcc cttaatggtg
     2281 tctccaagcc tgaagctgtg attttcccag tcagcgactg gaatcctcaa gaccagcctc
     2341 agaaggagga agacttgggc ccagctgtcc accatgtcta cgagctcatc aaccaggggc
     2401 ccagctccat cagccagggt gtgctggagc tcagctgtcc acaggctctg gaaggccaac
     2461 agctcctcta tgtgaccaag gtgacaggac tcagcaactg cacctccaac tacaccccca
     2521 actcacaggg cctggagttg gatccagaga cctctccaca ccacctgcag aaacgagagg
     2581 ctccagggag gagttctact gcctcaggaa cacaagttct gaaatgccct gaagccaagt
     2641 gtttcaggct gcgctgtgag tttgggccac tgcaccgcca agagagccgt agtctgcagc
     2701 tgcatttccg agtctgggcc aagaccttct tgcagcggga ataccagcca tttagccttc
     2761 agtgtgaggc tgtatatgaa gctctgaaga tgccctacca gatcctgcct cggcagcttc
     2821 cccaaaagaa acttcaggtg gccacagccg tgcagtggac caaggcagaa ggcagcaatg
     2881 gtgtcccgtt gtggatcatc atcctagcca ttctttttgg cctcctgctc ctaggtctgc
     2941 tcatctacgt cctctacaag ctcggcttct tcaaacgttc cctcccctac ggcacagcca
     3001 tggaaaaagc tcagctcaag cctccagcca cctcagatgc cggtaccagt actgggagtg
     3061 gggccaccaa cttttctctg ctcaagcaag ccggggatgt tgaagaaaat ccagggccaa
     3121 tggtgtctaa gggcgcagaa ctgttcaccg gaatcgtacc tattttgata gaacttaatg
     3181 gggatgtcaa tggacataaa ttcagtgtca gcggtgaggg tgaaggcgat gcgacttatg
     3241 ggaaactgac gctcaaattt atctgtacca ccggtaaact ccccgttccg tggccgaccc
     3301 ttgtcaccac cctgtcctat ggcgttcagt gttttagcag gtatccagat cacatgaaac
     3361 agcatgactt tttcaagtcc gccatgcctg agggttacat tcaagagcga acgatattct
     3421 ttgaagacga tggcaactat aagtccaggg cggaggtcaa gtttgagggc gacacgcttg
     3481 tgaataggat tgagcttacg gggactgatt ttaaagaaga cggcaatatc cttgggaata
     3541 aaatggaata caactataat gcccacaacg tgtatataat gacggataag gccaagaacg
     3601 gaatcaaagt caactttaaa atcagacaca acatcgaaga tggcagcgtg caacttgccg
     3661 accattacca gcaaaacacg cctattggcg acggtcctgt cttgctccct gacaatcact
     3721 atctcagcac gcaaagtgcc ctgagtaaag atcctaacga gaaacgggat cacatgattt
     3781 actttgggtt tgtgacagcg gctgctataa cgcatggtat ggacgaattg tacaaatgat
     3841 aaagtacttg ataagctctt caggttgagg tctctaaaag cgtcttcctg ttctcatcac
     3901 atcatatcaa ggttatatac catcaatatt gccacagatg ttacttagcc ttttaatatt
     3961 tctctaattt agtgtatatg caatgatagt tctctgattt ctgagattga gtttctcatg
     4021 tgtaatgatt atttagagtt tctctttcat ctgttcaaat ttttgtctag ttttattttt
     4081 tactgatttg taagacttct ttttataatc tgcatattac aattctcttt actggggtgt
     4141 tgcaaatatt ttctgtcatt ctatggcctg acttttctta atggtttttt aattttaaaa
     4201 ataagtctta atattcatgc aatctaatta acaatctttt ctttgtggtt aggactttga
     4261 gtcataagaa atttttctct acactgaagt catgatggca tgcttctata ttattttcta
     4321 aaagatttaa agttttgcct tctccattta gacttataat tcactggaat ttttttgtgt
     4381 gtatggtatg acatatgggt tcccttttat tttttacata taaatatatt tccctgtttt
     4441 tctaaaaaag aaaaagatca tcattttccc attgtaaaat gccatatttt tttcataggt
     4501 cacttacata tatcaatggg tctgtttctg agctctactc tattttatca gcctcactgt
     4561 ctatccccac acatctcatg ctttgctcta aatcttgata tttagtggaa cattctttcc
     4621 cattttgttc tacaagaata tttttgttat tgtctttggg ctttctatat acattttgaa
     4681 atgaggttga caagttaata acaggcctat tgattggaaa gtttgtcaac gaattgtggg
     4741 tcttttgggg tttgctgccc cttttacgca atgtggatat cctgctttaa tgcctttata
     4801 tgcatgtata caagcaaaac aggcttttac tttctcgcca acttacaagg cctttctcag
     4861 taaacagtat atgacccttt accccgttgc tcggcaacgg cctggtctgt gccaagtgtt
     4921 tgctgacgca acccccactg gttggggctt ggccataggc catcagcgca tgcgtggaac
     4981 ctttgtgtct cctctgccga tccatactgc ggaactccta gccgcttgtt ttgctcgcag
     5041 caggtctgga gcaaacctca tcgggaccga caattctgtc gtactctccc gcaagtatac
     5101 atcgtttcca tggctgctag gctgtgctgc caactggatc ctgcgcggga cgtcctttgt
     5161 ttacgtcccg tcggcgctga atcccgcgga cgacccctcc cggggccgct tggggctcta
     5221 ccgcccgctt ctccgtctgc cgtaccgtcc gaccacgggg cgcacctctc tttacgcgga
     5281 ctccccgtct gtgccttctc atctgccgga ccgtgtgcac ttcgcttcac ctctgcacgt
     5341 cgcatggagg ccaccgtgaa cgcccaccgg aacctgccca aggtcttgca taagaggact
     5401 cttggacttt cagcaatgtc atctcagtgg ctaataaagg aaatttattt tcattgcaat
     5461 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5521 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5581 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5641 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5701 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5761 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatctttgca
     5821 tttttagaca tttagaagcc tatatcttgt tacagaattg gaattacaca aaaattctac
     5881 catattttga aagcttaggt tgttctgaaa aaaacaatat attgttttcc tgggtaaact
     5941 aaaagtcccc tcgaggaaag gcccctaaag tgaaacagtg caaaacgttc aaaaactgtc
     6001 tggcaataca agttccactt tgaccaaaac ggctggcagt aaaagggtta agaagactgt
     6061 cagccttgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6601 ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc
     6841 gtaactcacg ttaagggatt ttggtcatga gttagaaaaa ctcatcgagc atcaaatgaa
     6901 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     6961 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     7021 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     7081 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     7141 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     7201 catcaaccaa accgttattc attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc
     7261 tgttaaaagg acaattacaa acaggaatcg agtgcaaccg gcgcaggaac actgccagcg
     7321 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaac gctgtttttc
     7381 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     7441 tcggaagtgg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     7501 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     7561 agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     7621 aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttgg atatggctca
     7681 ttttttactt cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat
     7741 tgtcgacact gcgggggctc tgtgtggtaa gcaggtctta acctttttac tgccaatgac
     7801 gcatgggata cgtcgtggca gtaaaagggc ttaaatgcca acgacgcgtc ccatacgttg
     7861 ttggcatttt aattcttctc tctgcagcgg cagcatgtgc cgccgctgca gagagtttct
     7921 agcgatgaca gcccctctgg gcaacgagcc gggggggctg tctttcttta tgttttaaat
     7981 gcactgacct cccacattcc ctttttagta aaatattcag aaataattta aatacatcat
     8041 tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc ccttcataat
     8101 atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga aattggacag
     8161 caagaaagcg agtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     8221 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     8281 cgcggatgtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     8341 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gacacgccga cggtggccag
     8401 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     8461 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     8521 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     8581 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     8641 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     8701 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     8761 gtactcggtc atggtggcgg acgaaaggcc cggagatgag gaagaggaga acagcgcggc
     8821 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
     8881 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
     8941 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca
     9001 ttgctcagcg gtgctgtcca tctgcacgag actagtgagt cgtgctactt ccatttgtca
     9061 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
     9121 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
     9181 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
     9241 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagagaa acttgatctg
     9301 tcgccgcaat tcaaacttcg tgaggctccg gtgcccgtca gtgacctgct atactctgga
     9361 gacgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     9421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     9481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     9541 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     9601 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     9661 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     9721 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     9781 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
     9841 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
     9901 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     9961 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
    10021 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
    10081 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
    10141 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
    10201 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
    10261 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
    10321 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
    10381 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
    10441 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
    10501 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
    10561 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
    10621 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
    10681 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
    10741 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
    10801 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
    10861 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
    10921 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
    10981 gttattgtgc tgtctcatca ttttggcaaa gaattcggct tgatcgaagc cgtctcaggg
    11041 gagagaccac acccaagctg tctagagccg ccaccatgcc tttgctcctc ctgttgccct
    11101 tgctctggg
//
