LOCUS       sequence-432384-       11124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse integrin av expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Yuxin Hao, Jiabin Yan, Courtney Fraser, Aiping Jiang, Murali 
            Anuganti, Roushu Zhang, Kenneth Lloyd, Joseph Jardine, Jessica 
            Coppola, Rob Meijers, Jing Li, Timothy A.
  TITLE     Synthetic integrin antibodies discovered by yeast display reveal 
            alpha-V subunit pairing preferences with beta subunits
  JOURNAL   bioRxiv 2024.01.26.577394
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.26.577394"
COMMENT     Sequence Label: mouse av full length
FEATURES             Location/Qualifiers
     source          1..11124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3078..3134
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    5448..5503
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5502..5521)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6213..6801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6293..6312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6887..7696)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7610..7629
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7715..7762)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(8188..8787)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8271..8291)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8768..8787
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8797..9296)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8845..8864)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9238..9261
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        9380..9683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          9961..10977
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10901..10923
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10985..11004
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ctggcgccct cgcgttcaac ctggacgtcg aaagtcccgc cgagtatgcg ggtcccgagg
       61 gaagttactt cggattcgcc gtggacttct tcgagcccag cacgtcctcc aggatgtttc
      121 tcctggtggg agcccccaaa gcgaacacga cccagcctgg gattgtagaa ggagggcaag
      181 ttctcaaatg tgaatgctca tccagccgcc ggtgccagcc cattgagttt gattcaacag
      241 gcaatcgaga ttatgccaaa gatgacccac tggagtttaa gtcccaccag tggtttggag
      301 cctctgtgag gtcaaagcag gataaaatct tggcctgtgc tccattgtac cactggagga
      361 ctgagatgaa gcaggagaga gagccagttg gaacctgctt tcttcaggac ggcacaaaga
      421 ccgttgagta tgctccatgc aggtcaaaaa atattgatgc tgatggccag ggattttgtc
      481 aaggaggatt cagcattgat tttactaaag ctgacagagt acttctcggt ggtcctggta
      541 gcttttattg gcaaggtcag ctcatttcgg accaagtggc agaaatcata tctaaatatg
      601 acccaaatgt ctacagcatc aaatataata accaattagc aacacggact gcacaagcaa
      661 tttttgatga cagttatttg ggttactctg tggccgtggg agacttcaat ggtgacggca
      721 ttgaagattt tgtctcagga gttccaagag cagcaaggac tttgggaatg gtttatattt
      781 atgatgggaa aaatatgtcc tctttacaca attttactgg tgaacagatg gctgcgtatt
      841 ttggattttc tgtagctgct actgacatta atggggatga ttacgcagat gtgtttattg
      901 gagcccccct gttcatggac cgaggttccg atgggaaact ccaggaggtt ggccaggtct
      961 cagtgtctct gcagagagca gtgggagact tccagactac aaagctgaac ggctttgagg
     1021 tttttgccag gtttggaagt gccatagctc cattgggaga cctggaccag gatggcttca
     1081 atgatattgc aattgctgct ccctatggtg gtgaagataa gaagggactt gtttatatct
     1141 tcaatggaag gtccacaggc ttgaattcgg tgccatctca aatcctcgag gggcagtggg
     1201 ctgctcagag catgccccca agctttggct attcaatgaa gggagctaca gatgtagaca
     1261 ggaatggata tccagactta gttgtaggag cttttggtgt ggatcgagct gtcttataca
     1321 gagccagacc cgttgtcact gtaaatgctg gccttgaagt gtaccctagc attttaaatc
     1381 aagacaataa gatctgcccg ttgcctggga cagctctcaa ggtttcctgt tttaatgtca
     1441 gattctgctt aaaggcagat ggcaagggaa cccttcctcg gaagctccac ttccaggtgg
     1501 agcttctact ggataaactc aagcagaagg gagccatccg acgagcactg tttctccata
     1561 acaggtcccc ggtccactcc aagaccatga ctgttttcag ggggggacag atgcagtgtg
     1621 aggaactggt cgcctatctt cgggatgaat ctgaatttag agacaagctc actcccatca
     1681 ccattttcat ggagtatcgg ttggaccaga gaacggctgc tgatgccaca ggcttgcagc
     1741 ccatcctgaa ccagttcacg ccggccaacg tcagtcggca ggctcatatt ctacttgact
     1801 gtggtgaaga caatgtctgt aaacctaaat tggaagtttc tgtaaatagt gatcaaaaga
     1861 agatctatat tggggatgac aaccctctga cactgactgt gaaggcgcag aatcaagggg
     1921 aaggtgccta tgaagctgag ctcatcgttt ctatcccgcc gcaggccgac ttcatcgggg
     1981 ttgtccggaa caacgaagcc ttagcaagac tgtcctgtgc atttaagaca gaaaaccaaa
     2041 ctcggcaggt ggtgtgcgac cttggaaacc caatgaaggc tggaactcaa ctgctcgctg
     2101 gccttcgttt cagcgtgcac cagcagtcag agatggatac ttctgtgaag tttgacttga
     2161 aaatccagag ctctaattcc tttgacaatg taagcccagt tgtgtcttac aaggttgacc
     2221 tcgccgtttt ggctgctgtg gagataagag gagtttcaag tccagatcat atctttcttc
     2281 caattccaaa ttgggagtac aaggaaaacc ctgagactga agaagacgtt gggcctattg
     2341 ttcagcacat atatgagctg agaaacaatg gtccaagttc attcagcaag gcaattctaa
     2401 atctccagtg gccttacaaa tacaacaaca acactctgtt gtacattctt cattacgaca
     2461 ttgacgggcc aatgaactgc acggcagata cagagatcaa ccctttgaga atcaagacac
     2521 ccgaaaagaa tgacacaggg gcggcgggac agggagaaag gaatcatctc atcaccaagc
     2581 gggatctcac cctcagagag ggagatgttc acactttggg ctgtggaatc gccaagtgct
     2641 tgcagatcac ctgccaggtt ggtcgactgg atagaggcaa gagcgcaatc ctgtacgtga
     2701 aatccctgct gtggaccgag acgttcatga acaaggagaa ccagaaccat tcctattcgc
     2761 tgaagtcatc tgcttccttt aatatcatag aattccctta caagaacctg ccgatcgagg
     2821 atctcttcaa ctctacacta gtcaccacta acatcacctg gggcattcag ccggcgccca
     2881 tgcctgtgcc tgtgtgggtg atcatcttgg cagttctcgc agggctgctg ctactggctg
     2941 tgttggtatt tgtaatgtac aggatgggct ttttcaaacg tgtccgacca cctcaagaag
     3001 agcaagaaag agaacagctt cagcctcatg agaatggcga aggaaactct gaaaccggta
     3061 ccagtactgg gagtggggcc accaactttt ctctgctcaa gcaagccggg gatgttgaag
     3121 aaaatccagg gccaatggtg tctaagggcg cagaactgtt caccggaatc gtacctattt
     3181 tgatagaact taatggggat gtcaatggac ataaattcag tgtcagcggt gagggtgaag
     3241 gcgatgcgac ttatgggaaa ctgacgctca aatttatctg taccaccggt aaactccccg
     3301 ttccgtggcc gacccttgtc accaccctgt cctatggcgt tcagtgtttt agcaggtatc
     3361 cagatcacat gaaacagcat gactttttca agtccgccat gcctgagggt tacattcaag
     3421 agcgaacgat attctttgaa gacgatggca actataagtc cagggcggag gtcaagtttg
     3481 agggcgacac gcttgtgaat aggattgagc ttacggggac tgattttaaa gaagacggca
     3541 atatccttgg gaataaaatg gaatacaact ataatgccca caacgtgtat ataatgacgg
     3601 ataaggccaa gaacggaatc aaagtcaact ttaaaatcag acacaacatc gaagatggca
     3661 gcgtgcaact tgccgaccat taccagcaaa acacgcctat tggcgacggt cctgtcttgc
     3721 tccctgacaa tcactatctc agcacgcaaa gtgccctgag taaagatcct aacgagaaac
     3781 gggatcacat gatttacttt gggtttgtga cagcggctgc tataacgcat ggtatggacg
     3841 aattgtacaa atgataaagt acttgataag ctcttcaggt tgaggtctct aaaagcgtct
     3901 tcctgttctc atcacatcat atcaaggtta tataccatca atattgccac agatgttact
     3961 tagcctttta atatttctct aatttagtgt atatgcaatg atagttctct gatttctgag
     4021 attgagtttc tcatgtgtaa tgattattta gagtttctct ttcatctgtt caaatttttg
     4081 tctagtttta ttttttactg atttgtaaga cttcttttta taatctgcat attacaattc
     4141 tctttactgg ggtgttgcaa atattttctg tcattctatg gcctgacttt tcttaatggt
     4201 tttttaattt taaaaataag tcttaatatt catgcaatct aattaacaat cttttctttg
     4261 tggttaggac tttgagtcat aagaaatttt tctctacact gaagtcatga tggcatgctt
     4321 ctatattatt ttctaaaaga tttaaagttt tgccttctcc atttagactt ataattcact
     4381 ggaatttttt tgtgtgtatg gtatgacata tgggttccct tttatttttt acatataaat
     4441 atatttccct gtttttctaa aaaagaaaaa gatcatcatt ttcccattgt aaaatgccat
     4501 atttttttca taggtcactt acatatatca atgggtctgt ttctgagctc tactctattt
     4561 tatcagcctc actgtctatc cccacacatc tcatgctttg ctctaaatct tgatatttag
     4621 tggaacattc tttcccattt tgttctacaa gaatattttt gttattgtct ttgggctttc
     4681 tatatacatt ttgaaatgag gttgacaagt taataacagg cctattgatt ggaaagtttg
     4741 tcaacgaatt gtgggtcttt tggggtttgc tgcccctttt acgcaatgtg gatatcctgc
     4801 tttaatgcct ttatatgcat gtatacaagc aaaacaggct tttactttct cgccaactta
     4861 caaggccttt ctcagtaaac agtatatgac cctttacccc gttgctcggc aacggcctgg
     4921 tctgtgccaa gtgtttgctg acgcaacccc cactggttgg ggcttggcca taggccatca
     4981 gcgcatgcgt ggaacctttg tgtctcctct gccgatccat actgcggaac tcctagccgc
     5041 ttgttttgct cgcagcaggt ctggagcaaa cctcatcggg accgacaatt ctgtcgtact
     5101 ctcccgcaag tatacatcgt ttccatggct gctaggctgt gctgccaact ggatcctgcg
     5161 cgggacgtcc tttgtttacg tcccgtcggc gctgaatccc gcggacgacc cctcccgggg
     5221 ccgcttgggg ctctaccgcc cgcttctccg tctgccgtac cgtccgacca cggggcgcac
     5281 ctctctttac gcggactccc cgtctgtgcc ttctcatctg ccggaccgtg tgcacttcgc
     5341 ttcacctctg cacgtcgcat ggaggccacc gtgaacgccc accggaacct gcccaaggtc
     5401 ttgcataaga ggactcttgg actttcagca atgtcatctc agtggctaat aaaggaaatt
     5461 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     5521 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     5581 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     5641 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     5701 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     5761 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     5821 atggagatct ttgcattttt agacatttag aagcctatat cttgttacag aattggaatt
     5881 acacaaaaat tctaccatat tttgaaagct taggttgttc tgaaaaaaac aatatattgt
     5941 tttcctgggt aaactaaaag tcccctcgag gaaaggcccc taaagtgaaa cagtgcaaaa
     6001 cgttcaaaaa ctgtctggca atacaagttc cactttgacc aaaacggctg gcagtaaaag
     6061 ggttaagaag actgtcagcc ttgagcggta tcagctcact caaaggcggt aatacggtta
     6121 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6181 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6241 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6301 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6361 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6421 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6481 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6541 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6601 ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta
     6661 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6721 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6841 tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagttag aaaaactcat
     6901 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     6961 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     7021 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     7081 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     7141 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     7201 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc
     7261 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgagtgc aaccggcgca
     7321 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     7381 ggaacgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     7441 taaaatgctt gatggtcgga agtggcataa attccgtcag ccagtttagt ctgaccatct
     7501 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     7561 cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     7621 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc
     7681 gttggatatg gctcattttt tacttcctca ccttgtcgta ttatactatg ccgatatact
     7741 atgccgatga ttaattgtcg acactgcggg ggctctgtgt ggtaagcagg tcttaacctt
     7801 tttactgcca atgacgcatg ggatacgtcg tggcagtaaa agggcttaaa tgccaacgac
     7861 gcgtcccata cgttgttggc attttaattc ttctctctgc agcggcagca tgtgccgccg
     7921 ctgcagagag tttctagcga tgacagcccc tctgggcaac gagccggggg ggctgtcttt
     7981 ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata ttcagaaata
     8041 atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat ccagatgctc
     8101 aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa ggaaccttta
     8161 atagaaattg gacagcaaga aagcgagtca ggcaccgggc ttgcgggtca tgcaccaggt
     8221 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     8281 ggggaggttg cggggcgcgg atgtctccag gaaggcgggc accccggcgc gctcggccgc
     8341 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgacac
     8401 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     8461 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     8521 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     8581 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     8641 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     8701 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     8761 gcgcaccgtg ggcttgtact cggtcatggt ggcggacgaa aggcccggag atgaggaaga
     8821 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     8881 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     8941 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     9001 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagtcgtgc
     9061 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     9121 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     9181 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     9241 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     9301 gagaaacttg atctgtcgcc gcaattcaaa cttcgtgagg ctccggtgcc cgtcagtgac
     9361 ctgctatact ctggagacgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9421 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9481 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9541 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9601 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9661 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     9721 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     9781 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg sggggsgggg sgrggggsgg
     9841 ggsggggsgr ggsggagagg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     9901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     9961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
    10021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
    10081 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
    10141 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
    10201 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
    10261 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
    10321 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
    10381 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
    10441 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
    10501 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg kgggggtgcc gggcggggcg
    10561 gggccgcckc gggccggggr gggctcgggg grggggcgcg gcggcccccg gagcgccggc
    10621 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
    10681 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
    10741 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
    10801 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
    10861 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
    10921 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
    10981 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cggcttgatc
    11041 gaagccgtct caggggagag accacaccca agctgtctag agccgccacc atgcctttgc
    11101 tcctcctgtt gcccttgctc tggg
//
