LOCUS       sequence-432395-       12468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express 
            HaloTag-MDC1 in human cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Heyza JR, Mikhova M, Bahl A, Broadbent DG, Schmidt JC
  TITLE     Systematic analysis of the molecular and biophysical properties of 
            key DNA damage response factors.
  JOURNAL   Elife. 2023 Jun 21;12:e87086. doi: 10.7554/eLife.87086.
  PUBMED    37341699
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-21"; volume: "12:e87086. doi"; pages: " 
            10.7554/eLife.87086"
COMMENT     Sequence Label: Halo-MDC1
FEATURES             Location/Qualifiers
     source          1..12468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1070..1135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1136..1169
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1178..2068
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2081..2101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(8419..8437)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    8469..8590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8480..8499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8534..8553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8835..9192
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8835..9026
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8858..8878)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9043..9178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9243..10037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9297..9316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9907..9926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10101..10149
                     /note="synthetic polyadenylation signal"
     promoter        10305..10409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10410..10478,10479..11270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10628..10647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(11428..12016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11508..11527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    12132..12415
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg actacaaaga
     1081 ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg acaagataac
     1141 ttcgtataat gtatgctata cgaagttatc cggtaccatg gcagaaatcg gtactggctt
     1201 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     1261 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     1321 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     1381 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     1441 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     1501 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     1561 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     1621 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1681 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1741 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1801 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1861 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1921 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     1981 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     2041 ctggctgtcg acgctcgaga tttccggcga gccaaccact gaggatctgt actttcagag
     2101 cgataacgcg atcgcttcca tggaggacac ccaggctatt gactgggatg ttgaagaaga
     2161 ggaggagaca gagcaatcca gtgaatcctt gaggtgtaac gtggagccag tagggcggct
     2221 acatatcttt agtggtgccc atggaccaga aaaagatttc ccactacacc tcgggaagaa
     2281 tgtggtaggc cgaatgcctg actgctctgt ggccctgccc tttccatcta tctccaaaca
     2341 acatgcagag attgaaatct tagcctggga caaggcacct atcctccgag actgtgggag
     2401 ccttaatggt actcaaatcc tgagacctcc taaggttttg agccctgggg tgagtcaccg
     2461 tctgagggac caggaattga ttctctttgc tgacttgctc tgccagtacc atcgcctgga
     2521 tgtctctctg ccctttgtct cccggggccc tctgacagta gaagagacac ccagagtaca
     2581 gggagaaact caaccccaga ggcttctgtt ggctgaggac tcggaggagg aagtagattt
     2641 tctttctgaa aggcgtatgg taaaaaaatc aaggaccaca tcttcctctg tgatagttcc
     2701 agagagtgat gaagaggggc attccccggt cctgggcggc cttgggccgc cttttgcctt
     2761 caatttgaac agtgacacag atgtggaaga aggtcagcaa ccagccacag aggaggcctc
     2821 ctcagctgcc agaagaggtg ccactgtaga ggcaaagcag tctgaagctg aagttgtaac
     2881 tgaaatccag cttgaaaagg atcagccttt agtgaaggag agggacaatg atacaaaagt
     2941 caagaggggt gcagggaatg gggtggttcc agctggggtg attctggaga ggagccaacc
     3001 tcctggagag gacagtgaca cagatgtgga tgatgacagc aggcctcctg gaaggccagc
     3061 tgaggtccat ttggaaaggg ctcagccttt tggcttcatc gacagcgaca ctgatgcgga
     3121 agaagagagg atcccagcaa ccccagttgt cattcctatg aagaagagga agatcttcca
     3181 tggagtaggt acaaggggtc ctggagcacc aggcctggcc catctgcagg agagccaggc
     3241 tggtagtgat acagatgtgg aagaaggcaa ggccccacag gctgtccctc tggagaaaag
     3301 ccaagcttcc atggttatca acagcgatac agatgacgag gaagaagtct cagcagcgct
     3361 gactttggca catctgaaag agagccagcc tgctatatgg aacagagatg cagaagagga
     3421 catgccccaa cgtgtggtcc ttctgcagcg aagccaaacc accactgaga gagacagtga
     3481 cacagacgtg gaggaggaag agctcccagt ggaaaataga gaagctgtcc tcaaggatca
     3541 cacaaagatt agagcccttg ttagagcaca ttcagaaaag gaccaacctc cttttgggga
     3601 cagtgatgac agtgtggaag cagataagag ctcacctggg atccacctgg agagaagcca
     3661 agcctccacc acagtggaca tcaacacaca agtggagaag gaagtcccgc cagggtcagc
     3721 cattatgcat ataaagaagc atcaggtgtc tgtggagggg acaaatcaaa cagatgtgaa
     3781 agcagttggg ggaccagcaa agctgcttgt ggtatctcta gaggaagcct ggcctctgca
     3841 tggggactgt gaaacagatg cagaggaggg cacctcccta acagcctcag tagttgcaga
     3901 tgtaagaaag agccagcttc cagcagaagg ggatgctggg gcagagtggg ctgcagctgt
     3961 tcttaagcag gagagagctc atgaggtggg ggcccagggt gggccacctg tggcacaagt
     4021 ggagcaggac ctccctatct caagagagaa cctcacagat ctggtggtgg acacagacac
     4081 tctaggggaa tccacccagc cacagagaga gggagcccag gtccccacag gaagggagag
     4141 agaacaacat gtgggtggga ccaaggactc tgaagacaac tatggtgatt ctgaagatct
     4201 ggacctacaa gctacccagt gctttctgga gaatcagggc ctggaagcag tccagagcat
     4261 ggaggatgaa cctacccagg ccttcatgtt gactccaccc caagagcttg gcccttccca
     4321 ttgcagcttc cagacaacag gtaccctaga tgaaccatgg gaggtcctgg ctacacagcc
     4381 attctgtctg agagagtctg aggactctga gacccagcct tttgacacgc accttgaggc
     4441 ctatggacct tgcctgtctc cacctagggc aataccagga gaccaacatc cagagagccc
     4501 agttcacaca gagccaatgg ggattcaagg cagagggagg cagactgtgg ataaagtcat
     4561 gggtatacca aaagaaacag cagagagggt gggccctgag agagggccat tggagagaga
     4621 aactgagaaa ctgctaccag aaagacagac agatgtgaca ggagaggaag aattaaccaa
     4681 ggggaaacag gacagagaac aaaaacagtt gttagctaga gacacccaga gacaagaatc
     4741 tgacaaaaat ggggaaagtg caagtcctga aagagatagg gagagtttga aggtagaaat
     4801 tgagacatct gaggaaatac aagagaaaca agtacagaag cagacccttc caagcaaagc
     4861 atttgagaga gaagtagaga gaccagtagc aaacagagag tgcgatccag ccgagttaga
     4921 agagaaggtg cccaaagtga tcctggagag agatacacag agaggggagc cagagggagg
     4981 gagccaggac cagaaagggc aggcctccag cccaacacca gagcctgggg tgggggcggg
     5041 ggaccttccg ggacctacct cagcccccgt accttctggg agccagtcag gtggaagggg
     5101 atccccagtg agccccagga ggcatcagaa aggcctcctg aattgcaaga tgccacctgc
     5161 tgagaaggct tccaggatca gagctgctga gaaggtttcc aggggcgatc aggaatctcc
     5221 agatgcttgt ctgcctcctg cagtacctga agccccagcc ccaccccaaa agccccttaa
     5281 ctctcagagc cagaaacatc ttgcacctcc gccccttctt tctccccttt taccttctat
     5341 caagccaacc gttcgtaaga ccaggcaaga tgggagtcag gaagctccag aggctccctt
     5401 gtcctcagag ctggagcctt tccacccaaa gcctaaaatt agaactcgga agtcctccag
     5461 aatgacaccc tttccagcta cctctgctgc ccctgagccc cacccttcca cctccacagc
     5521 ccagccagtc actcccaagc ccacatctca ggccactagg agcaggacaa ataggtcctc
     5581 tgtcaagacc cctgaaccag ttgtccccac agcccctgag ctccagcctt ccacctccac
     5641 agaccagcct gtcacctctg agcccacatc tcaggttact aggggaagaa aaagtagatc
     5701 ctctgtcaag acccctgaaa cagttgtgcc cacagccctt gagctccagc cttccacctc
     5761 caccgaccga cctgtcacct ctgaacccac ctctcaggct actaggggaa gaaaaaatag
     5821 atcctctgtc aagacccctg aaccagttgt ccccacagcc cctgagctcc agccttccac
     5881 ctccacagac cagcctgtca cttctgagcc cacatatcag gctactaggg gaagaaaaaa
     5941 tagatcctct gtcaagaccc ctgaaccagt tgtgcccaca gcccctgagc tccggccttc
     6001 cacctccaca gaccgacctg tcacccccaa gcccacatct cggaccacta ggagcaggac
     6061 aaatatgtcc tctgtcaaga cccctgaaac agttgtcccc acagcccctg agctccagat
     6121 ttccacctcc acagaccaac ctgtcacccc taagcccaca tctcggacca ctaggagcag
     6181 gacaaatatg tcctctgtga agaaccctga atcaactgtc cctatagccc ctgagctccc
     6241 accttccacc tccacagagc agcctgtcac ccctgagccc acatctcggg ctactagggg
     6301 aagaaaaaat agatcctctg gcaagacccc tgaaacactt gtccccacag cccctaagct
     6361 cgagccttcc acttccacag accaacctgt cactcctgag cccacatctc aggccaccag
     6421 gggcaggaca aataggtcct ctgtgaagac ccctgaaaca gttgtcccca cagcccctga
     6481 gctccagcct tccacctcca cagaccagcc tgttacccct gagcctacgt ctcaggctac
     6541 taggggaaga acagatagat cctctgtcaa gactcctgaa acagttgtcc ccacagcccc
     6601 tgagctacag gcttccgcct ccacagacca gcctgtcacc tctgagccca catctcggac
     6661 cactagggga agaaaaaatc ggtcctctgt caagacccct gaaacagttg tgcccgcagc
     6721 ccctgagctc cagcctccca cctccacaga ccgacctgtc acccctgagc ccacatctcg
     6781 ggccactagg ggcaggacaa ataggtcctc tgtcaagacc cctgaatcaa ttgtccctat
     6841 agcccctgag cttcagcctt ccacctccag aaaccagctt gtcacccctg agcccacatc
     6901 tcgggccact aggtgcagga caaataggtc ctctgtcaag acccctgagc cagttgtccc
     6961 cacagcccct gagccccatc ctaccacctc cacagaccag cctgtcaccc ccaagctcac
     7021 atctagggcc actaggagaa agacaaatag gtcctctgtc aagactccca aaccagttga
     7081 accagcagcc tctgatcttg agccttttac ccccacagac cagtccgtca cccctgaggc
     7141 catagctcag ggtggtcaga gcaaaacact gaggtcttcc acagtaagag ctatgccggt
     7201 tcctaccacc cctgaattcc aatctcctgt caccacagac cagcctattt cccctgagcc
     7261 tattactcaa cccagttgca tcaagaggca gagagccgct gggaaccctg gctccctcgc
     7321 agctcccatt gaccataagc cttgctctgc acccttggaa cctaaatccc aggcctcaag
     7381 gaaccaaaga tggggagcag tgagagcagc tgaatccctt acagccattc ctgagcctgc
     7441 ctctccccag cttcttgaga caccaattca tgcctcccag atccaaaagg tggaaccagc
     7501 aggtagatct aggttcaccc cggagctcca gcctaaggcc tctcaaagcc gcaagaggtc
     7561 tttagctacc atggattcac caccacatca aaaacagccc caaagagggg aagtctccca
     7621 gaagacagtg attatcaagg aagaggaaga agatactgca gagaagccag ggaaggaaga
     7681 ggatgtcgtg actccaaaac caggcaagag aaagagagac caggcagagg aggagcccaa
     7741 cagaatacca agccgcagcc tccgacggac caaacttaac caagaatcaa cagcccccaa
     7801 agtgctcttc acaggagtgg tggatgctcg gggagagcgg gctgtgctgg cactgggggg
     7861 aagtctggct ggttcagcgg cagaggcttc ccacctggtc actgatcgca tccgccggac
     7921 agtcaagttc ctgtgtgccc tggggcgggg aatccccatt ctgtccctgg actggctgca
     7981 tcagtcccgc aaggctggtt tcttcttacc cccggatgaa tatgtggtga ccgaccctga
     8041 gcaagagaag aactttggct ttagccttca agacgcactg agcagggctc gggagcgaag
     8101 gctgctagag ggctatgaga tctatgtgac ccctggagtc cagccaccac cacctcagat
     8161 gggagagatt attagctgct gtggaggcac atacctaccc agcatgcctc ggtcctataa
     8221 gcctcagaga gttgtgatca catgccctca ggacttccct cattgctcca ttccactacg
     8281 ggttgggctg cccctcctct cgcctgagtt cctgctgact ggagtgctga agcaggaagc
     8341 caagccagag gcctttgtcc tctccccttt ggagatgtca tccacctgag cggccgcttg
     8401 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggc cgcttcgagc
     8461 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     8521 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     8581 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     8641 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     8701 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     8761 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     8821 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     8881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8941 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9001 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9061 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9121 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9181 ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca
     9241 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9301 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9361 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9421 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9481 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9541 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9601 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9661 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9721 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     9781 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9841 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9901 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9961 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    10021 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    10081 cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
    10141 ttttgtgtga atcgatagcg ataaggatcc tctttgcgct tgcgttttcc cttgtccaga
    10201 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
    10261 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    10561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttca
    10621 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10801 ctgcggccaa cttacttctg acaactatcg gaggaccgaa ggagctaacc gcttttttgc
    10861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    11041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    11101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    11161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    11221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ttcgaaatga
    11281 ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    11881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12001 aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12121 ccttttatag tccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa
    12181 accatgaaaa atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg
    12241 ttgcataggt attcatacgg ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg
    12301 gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc
    12361 gtacaattaa gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa
    12421 accgcagtac tccggccacg atgcgtccgg cgtagaggat cgagatct
//
