LOCUS       sequence-432437-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MRGPRB2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Al Hamwi G, Namasivayam V, Buschbell B, Gedschold R, Golz S, Muller 
            CE
  TITLE     Proinflammatory chemokine CXCL14 activates MAS-related G 
            protein-coupled receptor MRGPRX2 and its putative mouse ortholog 
            MRGPRB2.
  JOURNAL   Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5.
  PUBMED    38184723
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-01-6"; volume: "7(1):52. doi"; pages: " 
            10.1038/s42003-023-05739-5"
COMMENT     Sequence Label: pLVX-IRES-MRGPRB2-mCherry
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2820..2846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3878..4450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3901..4452
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4045..4062)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4452..5162
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4597..4615)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4865..4884
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5176..5764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5229..5249)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5647..5658)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5971..6604
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6732..6748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6732..6748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6745..6767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6756..6772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6780..6786,6787..6804,6805..6810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6825..6846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6963..6980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7134..7722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7214..7233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7893..8684,8685..8753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8516..8535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8754..8858)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8906..9040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8943..8962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8997..9016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9208,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcctc gagaccatgt
     2821 acccctacga cgtgcccgac tacgcgaccg gtatgagtgg agatttccta atcaagaatc
     2881 taagcacctc agcctggaaa acgaacatca cagtgctgaa tggaagctac tacatcgata
     2941 cttcagtttg tgtcaccagg aaccaagcca tgattttgct ttccatcatc atttccctgg
     3001 ttgggatggg actaaatgcc atagtgctgt ggttcctggg catccgtatg cacacgaatg
     3061 ccttcactgt ctacattctc aacctggcta tggctgactt tctttacctg tgctctcagt
     3121 ttgtaatttg tcttcttatt gccttttata tcttctactc aattgacatc aacatccctt
     3181 tggttcttta tgttgtgcca atatttgctt atctttcagg tctgagcatt ctcagcacca
     3241 ttagcattga gcgctgcttg tctgtaatat ggcccatttg gtatcgctgt aaacgtccaa
     3301 gacacacatc agctatcaca tgttttgtgc tttgggttat gtccttattg ttgggtctcc
     3361 tggaagggaa ggcatgtggc ttactgttta atagctttga ctcttattgg tgtgaaacat
     3421 ttgatgttat cactaatata tggtcagttg ttttttttgg tgttctctgt gggtctagcc
     3481 tcaccctgct tgtcaggatc ttctgtggct cacagcgaat tcctatgacc aggctgtatg
     3541 tgactattac actcacagtc ttggtcttcc tgatctttgg tcttcccttt gggatctatt
     3601 ggatactcta tcagtggatt agcaattttt attatgttga aatttgtaat ttttatcttg
     3661 agatactatt cctatcctgt gttaacagct gtatgaaccc catcatttat ttccttgttg
     3721 gctccattag gcaccgaagg ttcaggcgga agactctcaa gctacttctg cagagagcca
     3781 tgcaagacac ccctgaggag gaacaaagtg gaaataagag ttcttcagaa caccctgaag
     3841 aactggaaac tgttcagagc tgcagctgag gatcccgccc ctctccctcc ccccccccta
     3901 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3961 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     4021 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     4081 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     4141 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     4201 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtaagttgg atagttgtgg
     4261 aaagagtcaa atggctcacc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     4321 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     4381 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4441 acgatgataa tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     4501 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     4561 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     4621 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     4681 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     4741 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     4801 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     4861 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     4921 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     4981 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     5041 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5101 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     5161 gaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5221 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5281 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5341 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5401 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5461 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5521 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5581 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5641 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5701 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5761 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     5821 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5881 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5941 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     6001 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     6061 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6121 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6181 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6241 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     6301 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6361 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     6421 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6481 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6541 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6601 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6661 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     6721 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6781 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7561 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8881 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9001 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     9061 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     9121 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     9181 ttaaatatgt actacaaact tagtagtt
//
