LOCUS       sequence-432469-        9689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ppdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and
            other neurons expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH4609
FEATURES             Location/Qualifiers
     source          1..9689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3149..3182)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      4059..4382
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(4386..4419)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7129..7151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7251..7270
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7348..7366)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7434..7538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7539..7607,7608..8399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7757..7776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8570..9158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9059..9078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9312..9329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9446..9467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9482..9487,9488..9505,9506..9512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9520..9536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9525..9547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9544..9560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9544..9560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgcaaaa ggttgcttta ccgagacgtg acgggacact
       61 ccttatcagc gatctaactt tgtatggaac ttttcacaac tctggatgct ggcgatcgta
      121 aatcgtactg ctccaaatgt ctagaacttt aaagacaata aaaagccgca aaagtatttg
      181 taaaaaatca aagaaaatag tttatatcga aaattaagta tcaagacctt tttagaagaa
      241 tggccttgta tgatttgtga gttgacatct tctgattcta aagatgcgta aactttgaaa
      301 ctgaataaac ccctctttca tattcaaaag acctaaattc ttgtaacaca acatccaaaa
      361 ttgtacgtga caactggaat aatgactgat tttcactata gcctcctatt tcggttgttt
      421 tgtttatttg tttattcatt aatattttca taataatgaa attatcgagg gtctttaaca
      481 cttttgagca catagagctc tcatgtgacg tcaaagttgg ttttgaaacc tcgtgtggta
      541 cttcttattc tttttttctt cggtcgatcg gaacaattca attaaaacta atgcacatac
      601 atcccctttt ctaccactcc accatcttct tcttctcttt ttcttcttcc aacacacgaa
      661 gattctgatt ctgattccat catgacgtgt tgatgaaaga aggaaaccaa gtagtagcag
      721 aagtaaaaag aggaggaaaa aagttttttg gaaggaaaaa ggaaaaagca atttgatttt
      781 ggagaagatt cttccccttc tcactaccat cccacaaaat ttcttgcgga ggcttaatct
      841 tttcctgcaa aataagataa attggatgcg cgaattccgt gtcatttgca caagggcaac
      901 agaaatcgag taccaagagt tcataaactt ttcttgtttc aatgtaaaaa caaatgaaca
      961 tttagacaga aattccaata aaccaccaaa tttgaatttc cttaaacatc tcatatatgg
     1021 acacggctaa caaggtcctc ttgcaaatat aataggtttc attcggaagt cataggacga
     1081 gctgaaaggg ttcccttgga agacaaaaat tattaaagac taaaaacgac tgctccaggt
     1141 aggtagacag gggaaggcat gtcaaagtga ttgcctataa cggaagtcct agaaaatgta
     1201 aaatattatt ctactgtttt gttttatttc attcgatttt caaacacttg tgaaggaatc
     1261 tcgaagtaaa attttctcga aacggaggtt gtagtacagt tggcaaaaag tgagtaactg
     1321 tcttcagtag ctgtattagg ctagataata aactaagctt tataatatct ggtcacccaa
     1381 attaaacaag attaaacgaa gcacaagaaa tggagaaaaa gcaagtaaag actcaggacc
     1441 aaaggaattc cccgcgaaac tagcagacca aaacaatata tctatctttc tctctagtaa
     1501 tttaaatttc atctgaaact tgtacatgac ctctcttcct tctccacgcc tacttcctcc
     1561 tttttttttc attgaggctt cccgagttga cgaagaatca tcatgtccat taattttaca
     1621 ttaaatatta aaaatgggaa aacggacatt tgtatctcgt catttatctt tcggaaactc
     1681 ccagatagta ttgctccttg atcttctaga aaacttcaag ctcaactaaa tttaccgtac
     1741 tcacgggtac ggtagctgag aattttcatg tatgaatgtt aggaccgccc atcgagaaca
     1801 ttcccataac ctgtccagct acaataatca tcttgaatgc tcaaaaagcg cccctttttt
     1861 caaacggttt aaaaagttta aaaagttcaa aaaatggttt taaattattt ttccttgaga
     1921 atttgacaaa gaataaggtt ttataaaaaa ctaattttgt tttcaaaaca agtaacttct
     1981 aaaggaaatc ctctttctcc agtattgaat attccagtga ctctatattt ctgagaattt
     2041 atttttgacc ttcgacattt tcaaaatatt ttctagtttc tcaaggcgtt cttttgtatc
     2101 aataagccaa acgtgtgtaa gtgtgtaagt ggaaggcaaa atatgttggt gtagaaaata
     2161 ctatacctcg gcacgagctg actagactca ggtaacaaaa agctgtaaaa acaagtgaat
     2221 tttgctacgc aaacctgttt aagactttct gaattaaaca ctctcaccaa atattttttt
     2281 taagattgaa gatacttgac gatctctttt tgaaactttc ataaaacttc ttgttcataa
     2341 aatctattgt acacccaaaa taaaatctct gtttatttca tcattgctca ctaaaaactc
     2401 tcccattgat ttgtcaatgc tttgattcta gttaattaga cttaaacaga agaggcaggg
     2461 gaaaaggaaa aaaggattag ggaattcttt gaaaccgctt tgtttgtggt cagagttcgt
     2521 gtcaatctga aaataattcc catgaaaatg acggaaaatg tgaagaacgg agaattaaat
     2581 ttcacctgta atttttaatg gtatatacga catgaattat gcatagcagg tttgttgatt
     2641 ttttcaaggt ctcgcctcta ggaattttgt ttcaagaatt gaacaacttt gtagaggaaa
     2701 aacatggaaa cagtagaaag ctagaatgtt ttaatgatca tatatatctt gtaaatgtaa
     2761 aaagtttaga ctttcaagaa agcaaatttt ctactcaaaa ctcaaaatat gaatacgatc
     2821 cggtggcaga tctttgttga attgttgaaa tgttggatac tttttgtttc ttccttgtga
     2881 ttttccaggt tatacaaact ttcctgattt ttgaagagtt tatcgataac atgatgtatt
     2941 ttcgaaagtt tgttgaaact catcaaagtt taccgcagtc gattgaaaat tgataagttc
     3001 atggccaaaa actgtgcaac cttcctcctt tgaagatttc aagtcttcag ctttccttgt
     3061 ctggcatgga gcactcctat tcctgaggtc gcagttgatt ttttgcaagt ttttcctcaa
     3121 tctcattaag attatcccac tacccgggat aacttcgtat agcatacatt atacgaagtt
     3181 atatggtctc caagggagag gagctcttca ccggagtcgt cccaatcctc gtcgagctcg
     3241 acggagacgt caacggacac aagttctccg tccgtggaga gggagaggga gacgccacca
     3301 acggaaagct caccctcaag ttcatctgca ccaccggaaa gctcccagtc ccagtaagtt
     3361 taaacatata tatactaact aaccctgatt atttaaattt tcagtggcca accctcgtca
     3421 ccaccctctc ccacggagtc cagtgcttcg cccgttaccc agaccacatg aagcagcacg
     3481 acttcttcaa gtccgccatg ccagagggat acgtccagga gcgtaccatc ttcttcaagg
     3541 acgacggaac ctacaagagt aagtttaaac atgattttac taactaacta atctgattta
     3601 aattttcagc ccgtgccgag gtcaagttcg agggagacac cctcgtcaac cgtatcgagc
     3661 tcaagggagt cgacttcaag gaggacggaa acatcctcgg acacaagctc gagtacaact
     3721 tcaactccca caacatctac atcatggccg tcaagcagaa gaacggaatc aaggtaagtt
     3781 taaacatgat tttactaact aactaatctg atttaaattt tcaggtcaac ttcaagatcc
     3841 gtcacaacgt cgaggacgga tccgtccagc tcgccgacca ctaccagcag aacaccccaa
     3901 tcggagacgg accagtcctc ctcccagact cccactacct ctccacccag tccgtcctct
     3961 ccaaggaccc aaacgagaag cgtgaccaca tggtcctcct cgagttccgt accgccgccg
     4021 gaatcaccct cggaatggac gagctctaca agtgataaat gcaaaatcct ttcaagcatt
     4081 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
     4141 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
     4201 aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc
     4261 cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta
     4321 ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag
     4381 tctcaataac ttcgtatagc atacattata cgaagttatg ctagcatggc ctcccaggtc
     4441 gtgtacggcg agtgggccag cacccacaca gagtgctaca acatgagcag gatcgacagc
     4501 accttcgtga gcctgctgca gctggtgtgg gccgtggtga gcggatgcca gaccatcttc
     4561 atgatcagca gggcccccaa ggtgccctgg gagagcgtgt acctgccctt cgtggagtcc
     4621 atcacatacg ccctggccag caccggcaat ggcacactgc agatgaggga cggcaggttc
     4681 tttccctggt ccaggatggc cagctggctg tgcacatgcc ccattatgct gggccagatc
     4741 tccaacatgg ctctggtgaa gtacaagagc atccctctga accccatcgc ccaggccgcc
     4801 agcattatca gggtggtgat gggcattacc gctaccatct cccccgccga gtacatgaag
     4861 tggctgttct tcttcttcgg cgctacctgc ctggtcttcg aatacagcgt ggtgttcacc
     4921 atcttccagg tgggactgta cggcttcgag tccgtgggca cccctctggc ccagaaggtg
     4981 gtggtgagga tcaagatgct gaggctgatc ttcttcatcg cctggaccat gttccccatc
     5041 gtgtggctga tttcccccac cggcgtgtgc gtgatccacg agaacgtgag cgccatcctg
     5101 tatctgctcg ccgacggcct gtgcaagaac acctacggcg tgatcctgtg gtccaccgcc
     5161 tggggagtgc tcgagggaaa gtgggacccg gcttgcctcc ctggccagga gaagcctgag
     5221 gccgacgacc ccttcggact gaaccacgag aagaatgccc cccccaacga cgaggtcaac
     5281 atcagaatgt tcggcagggc ggccgccgcg gtaccgggag gaggaaccat ggtctcaaag
     5341 ggtgaagaag ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag
     5401 ggatctgtca atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag
     5461 ggaacacaaa ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat
     5521 tatttaaatt ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct
     5581 ccacagttca tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat
     5641 ttgaaacttt cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga
     5701 ggagttgtta cagtgacgca agactcaagg taagtttaaa cagttcggta ctaactaacc
     5761 atacatattt aaattttcag cctccaagat ggagaattta tttataaagt caaacttcga
     5821 ggaacgaatt tcccctcgga tggatctgtt atgcagaaga agactatggg atgggaagct
     5881 tcaagtgaaa gaatgtaccc tgaagacggt gctcttaagg gagagattaa acaacgtctt
     5941 aaattgaaag atggaggaca ttacgatgct gaggtaagtt taaacatgat tttactaact
     6001 aactaatctg atttaaattt tcaggtgaag acaacttaca aagccaaaaa accagttcag
     6061 ctgccaggag cgtacaatgt taatattaaa ctggatatca cctcccacaa cgaggattac
     6121 actatcgttg agcaatatga aagagctgaa gggcggcact cgacaggtgg catggatgaa
     6181 ttgtataagt tctgctacga gaacgaggtc taagaattcc aactgagcgc cggtcgctac
     6241 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     6301 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     6361 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     6421 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     6481 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     6541 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     6601 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     6661 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     6721 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     6781 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     6841 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     6901 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     6961 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     7021 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
     7081 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7141 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7201 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     7261 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7321 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     7381 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7441 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7501 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7561 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7621 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7681 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7741 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7801 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7861 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7921 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7981 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8041 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8101 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8161 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8221 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8281 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8341 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8401 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8701 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9421 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9541 acacaggaaa cagctatgac catgattacg ccaagctgta agtttaaaca tgatcttact
     9601 aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca ctcacaacga
     9661 tggatacgct aacaacttgg aaatgaaat
//
