LOCUS       sequence-432475-        7980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker 
            TA, Romling U
  TITLE     The membrane-cytoplasmic linker defines activity of FtsH proteases 
            in Pseudomonas aeruginosa clone C.
  JOURNAL   J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622.
            Epub 2024 Jan 3.
  PUBMED    38176647
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2024-02"; volume: "300(2):105622. doi"; pages: " 
            10.1016/j.jbc.2023.105622. Epub 2024 Jan 3"
COMMENT     Sequence Label: pSG068
FEATURES             Location/Qualifiers
     source          1..7980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(260..793)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(982..987,988..1004,1005..1010))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1280..1301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1316..1321,1322..1339,1340..1346)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1354..1370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1359..1381
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1378..1394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1378..1394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1413..1433
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1415..1433
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1462..1478
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1463..1479
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1571..2449)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2476..2760
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2683..2702
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(4770..4789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4771..4789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4799..4815)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4808..4830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(5524..6186)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      6187..6958
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga
      241 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
      301 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
      361 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
      421 cggtggcaat gccctgcctc cggtgctctc cggagactgc gagatcatag atatagatct
      481 cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt
      541 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
      601 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
      661 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
      721 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
      781 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
      841 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
      901 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
      961 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     1021 gccttcatcc gtttccacgg tgtgcgtcca tgggcaaata ttatacgcaa ggcgacaagg
     1081 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1141 gaatgcttaa tgaattacaa cagtttttat gcatgcgccc aatacgcaaa ccgcctctcc
     1201 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1261 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1321 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     1381 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     1441 agctgggtac cgggcccccc ctcgaggtcg acggtatcga tgcataatgt gcctgtcaaa
     1501 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
     1561 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     1621 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     1681 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     1741 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     1801 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     1861 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     1921 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     1981 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     2041 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     2101 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     2161 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     2221 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     2281 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     2341 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     2401 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     2461 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     2521 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     2581 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     2641 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     2701 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     2761 acccgttttt ttgggctagc ttgcgaggaa cattgcccat ggcaaagaat ctgattctgt
     2821 ggctgatcat cgcggcggta ctggtgaccg tgatgaacaa tttctcgagc ccgagcgagc
     2881 cacagacgct gaactactcg gacttcatcc agcaggtcaa ggatggcaag gtcgagcgag
     2941 tgaccgtgga tggctatgtc atcaccggca agcgtagcga cggcgatacc ttcaagacca
     3001 tccgcccggc gatccaggac aacggcctga tcggcgacct ggtcaacaac aacgtcgtgg
     3061 tcgagggcaa gcagcccgaa cagcagagca tctggaccca gttgctggtc gccagcttcc
     3121 cgatcctggt gatcatcgcc gtcttcatgt tcttcatgcg ccagatgcag ggtggcggcg
     3181 gcggtcgcgg cggcccgatg agcttcggca agagcaaggc gcgcctgctg tccgaggacc
     3241 aggtcaagac caccttcgct gacgtcgccg gctgcgacga ggccaaggag gaggtcagcg
     3301 agctggtcga gttccttcgc gatccgggca agttccagcg cctgggcggc cgtatcccgc
     3361 gcggcgtgct gatggtcggc ccgcccggca ccggcaagac cctgctggcc aaggccatcg
     3421 ccggcgaagc caaggtgccg ttcttcacca tttccggttc cgacttcgtc gagatgttcg
     3481 tcggcgtggg cgcttcccgt gtccgcgaca tgttcgacca ggccaagaag catgcgccgt
     3541 gcatcatctt catcgacgag atcgacgccg tcggccgcca tcgcggcgcc ggcctgggcg
     3601 gtggtcacga cgagcgcgag cagaccctca accagttgct ggtggagatg gatggcttcg
     3661 agatgaacga tggcatcatc gtcatcgctg ccaccaaccg tccggacgtt ctggacccgg
     3721 cgctgctgcg tccgggccgc ttcgaccgcc aggtggtggt cggcttgccg gatatccgcg
     3781 ggcgcgaaca gatcctcaag gtgcacatgc gcaaggtgcc gctgggcgac catgtcgatc
     3841 ctgccgtcat cgcccgcggt acgcccggtt tctccggcgc cgacctggcc aacctggtca
     3901 acgaggcctc gctgttcgcc gcgcgctcca acaagcgcat cgtcgacatg cgcgagttcg
     3961 agctggccaa ggacaagatc atgatgggcg ccgagcgcaa gaccatggtc atgtccgaga
     4021 aggagaagcg caacaccgcc taccacgaag ccggccacgc catcgtcggt cgcctggtgc
     4081 cggagcatga cccggtctac aaggtgtcca tcattccgcg cggacgcgcc ctgggcgtga
     4141 ccatgttcct ccccgaggaa gatcgctaca gcctgtccaa gcgcgcgctg gaaagccaga
     4201 tctgctcgct gttcggcggc cgtatcgcgg aagagatgac cctcggcttc gaaggcgtca
     4261 ccactggcgc ttccaacgac atcatgcgtg ctacccagtt ggcgcggaac atggtgacca
     4321 agtggggtct gtccgagaag ctcggcccgc tgatgtacgc ggaggaggag ggcgaggtgt
     4381 tcctcggccg tagcgccggc agccagcacg ccaatgtgtc gggcgagacc gccaagatga
     4441 tcgaccagga agtccggcgc atcatcgacg actgctacgg taccgccaag cgactgctcg
     4501 acgagaaccg cgacaagctg gagatgatgg ccgacgcact gatgaagtac gaaaccatcg
     4561 actccgacca gatcgacgac atcatggccg gtcgcgtgcc gcgcgagccg cgtgactggc
     4621 agggtggttc gggtaccggc actccgccgg ccaacctgga agagtcgggc cgccgcgaga
     4681 acacccctcc catcggtggg ccggccggcg agcactgaga gccctgaatg agtctagagc
     4741 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4801 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4861 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4921 cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt
     4981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgtactgcg
     5041 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     5101 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     5161 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     5221 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     5281 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     5341 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     5401 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     5461 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa
     5521 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag
     5581 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt
     5641 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc
     5701 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag
     5761 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact
     5821 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac
     5881 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac
     5941 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt
     6001 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag
     6061 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc
     6121 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt
     6181 ggccatgctt atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag
     6241 ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac
     6301 agattttctt taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt
     6361 accccccttt tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc
     6421 tttggtgccc aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc
     6481 ctccggcaaa aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc
     6541 ccaaggtggc gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag
     6601 gcgggcgggc ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc
     6661 gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt
     6721 ccaaccggca agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa
     6781 aaaaattgat ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa
     6841 ggctgggtag ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca
     6901 tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc
     6961 gcggccatcg tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa
     7021 gcccggagag caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag
     7081 caaagtctag tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg
     7141 cccccgtcga gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca
     7201 tgcgatgcaa gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc
     7261 gcgagcgcga gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg
     7321 ccagcagcac cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca
     7381 aggacgctgt gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg
     7441 ccagccaaga acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt
     7501 acggggcgga tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga
     7561 ccgcgttcgt ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca
     7621 acaaagcgca gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc
     7681 tgcaacgggg catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg
     7741 ccctggagcg gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg
     7801 cctatgcacc gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg
     7861 aagccgtggc cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg
     7921 ccgccgcagg agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag
//
