LOCUS       sequence-432479-       11526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Nova in DRG neurons promotes the ""mature"" 
            splicing pattern observed in CNS neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Weyn-Vanhentenryck SM, Feng H, Ustianenko D, Duffie R, Yan Q, Jacko 
            M, Martinez JC, Goodwin M, Zhang X, Hengst U, Lomvardas S, Swanson 
            MS, Zhang C
  TITLE     Precise temporal regulation of alternative splicing during neural 
            development.
  JOURNAL   Nat Commun. 2018 Jun 6;9(1):2189. doi: 10.1038/s41467-018-04559-0.
  PUBMED    29875359
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-6"; volume: "9(1):2189. doi"; pages: " 
            10.1038/s41467-018-04559-0"
COMMENT     Sequence Label: Lenti-Nova1-GFP
FEATURES             Location/Qualifiers
     source          1..11526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..248
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(257..685)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..388)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     579..598
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    731..955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     944..961
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             987..1167
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1673..1689
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1692..2280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1798..1809
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2207..2227
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2321..3034,3035..3037,3038..3040))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2324..3034,3035..3037,3038..3040))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2366..2387)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2715..2734
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2974..2995
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3037..3046
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4665..5876)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4809..4828)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5941..6058
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6356..6400)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6585..6818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7311..7436
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7483..7663
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7681..7879)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7706..7726)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7881..8260
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8432..8451
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8526..8630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8631..8699,8700..9491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8849..8868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9662..10250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10151..10170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10404..10421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10538..10559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10574..10579,10580..10597,10598..10604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10612..10628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10617..10639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10636..10652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10636..10652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10701..10822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10712..10731)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10766..10785
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10952..11326)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(11345..11392)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(11440..11526,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(11454..11526,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(join(11508..11526,1))
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      301 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      361 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      421 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      481 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      541 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      601 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      661 cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc
      721 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
      781 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
      841 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
      901 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
      961 gctgatcagc gggtttaaac gggccctgct agagattttc cacactgact aaaagggtct
     1021 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     1081 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     1141 tcagatctgg tctaaccaga gagacccagt acagtccgga tgcagctctc gggccatgtg
     1201 atgaaatgct aggcggctgt caaacctcca ctctaatact tctctctccg ggtcatccat
     1261 cccatgcagg ctcacagggt gtaacaagcg ggtgttctct ccttcattgg cttcttctac
     1321 cttctcttgc tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgat
     1381 ccctggccct ggtgtgtagt tctgccaatc agggaagtag ccttgtgtgt ggtagatcca
     1441 cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     1501 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtacctg
     1561 aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc
     1621 attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt cacgaggtcg
     1681 acggtatcga tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg
     1741 aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt
     1801 gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc
     1861 gagcagccaa ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa
     1921 cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat
     1981 agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc
     2041 agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca
     2101 caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat
     2161 acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg
     2221 agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt
     2281 atcgataagc ttgatatcga attctcgagt cgcggccgct ttacttgtac agctcgtcca
     2341 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     2401 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     2461 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     2521 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     2581 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     2641 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     2701 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     2761 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     2821 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     2881 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     2941 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     3001 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtattccag
     3061 gattctcttc gacatctccg gcttgtttca gcagagagaa gtttgttgcg ccggatccac
     3121 ccactttctg aggattggca gcccgaactc cttgctcata tgtgatcctc tgtgtaatta
     3181 aatactgagc agcctgtgtt gcagctggtg ttccagtaat ggttaccttc cgattccttg
     3241 tgccaggtac gaattctccc tttttggaga tctgtatcct tgcaccagtc aactcctggt
     3301 attccactaa ggttttccct cctttgccaa gtattgcacc aactaagttt tctggaactg
     3361 ctatttcaac tacatccttt gatccatctg tggatttctc tgtccctaga atggcactgg
     3421 cagctagggg cgaggcagct ccaaagtatc cattggttgc agcagtagca gcagccaggc
     3481 tacctaatgc aaatgtcccc gccgttccac cagccgtgct gccgctggct gaggcttcac
     3541 tggcataggt ggccaacaaa ttggctgctg ctgctgctgg gttggcactg gcagctgcgg
     3601 cagccaaagc ccccgttgct gctgcttggc tgagacctaa acctaatgtg ttgagattat
     3661 atccatagct ggctaatgta ttaagtgcag aggtgatggc caccaggtca ttgcctgtga
     3721 agccagataa aactgctggg aaggccgcaa cgccagcaag gttagcatgt cctaatagcc
     3781 ctgcggctgc tgcggcagtt ggtaacactt cagcagtgtt tgcataagga gatccggttg
     3841 gattggaatt tgccactgga cctgtcacat tggcataact gatattgaga cagctgccac
     3901 tctgtggatc ctcttgtatc ttctggatga taagttcaac agcttttcgg ttttgttcag
     3961 gttctccact cacagtgaca accctctctt gcaagttgat cccatcgggt ttctgggaaa
     4021 gctgcaccca agcccctgac tgctccatta tagccttcac agtagcacct cccttcccta
     4081 ttatcagacc tgctgtgctg ttgggaacta taatctttac ctgattggct ctggaggtgg
     4141 tcatggggtc agatggagag gacttggtgg tagttgggga agatggcaat gtttgtttga
     4201 tgcgatcagg attaacggtg gtctgaggtt gtaggatact gactggttct gtcttggcaa
     4261 cattttgggg catttctcgg attttttctg caatgaatcc atgaactgcg ttcagcgctt
     4321 caattgttcc ctggatcaag caaaccctct cagtagtacc tggataaaaa tctttggatt
     4381 tagacagctt gatagtggct ccagtttctt tttgcaactg aacaattgtc tgtcctccct
     4441 tcccaattat agatccagca gcataactag gtatgagaac ctttagaaag tactggccgt
     4501 cttcgcccgt gttggtcctc ttggtgctgc cggcttcagg gggcgcttca agcggccttt
     4561 tccgcgagtc cggcgggtcc agatctatgg gaacccccgt atgggtcccg ttctgctgaa
     4621 tgggagctgc cgccattcta gagtcgacct gcagcccaag cttcgtctaa caaaaaagcc
     4681 aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt acatattgac
     4741 ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact cgccaacccc
     4801 gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca taaaaccgac
     4861 caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc
     4921 aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca
     4981 cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga cgtcacgaca
     5041 cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc gccataactg
     5101 ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg
     5161 tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca ttagcgaagg
     5221 cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca acgacctcac
     5281 agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt ttgctgcgct
     5341 ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag cccttggcgg
     5401 tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc
     5461 cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga accgcgaccc
     5521 aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg
     5581 gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc
     5641 gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa
     5701 aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt
     5761 cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc
     5821 gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat
     5881 ctggagccga cacgggttaa ttaaccaaac tggatctctg ctgtccctgt aataaacccg
     5941 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     6001 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     6061 gtggatgaat actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta
     6121 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
     6181 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
     6241 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
     6301 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
     6361 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
     6421 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
     6481 aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca ttccaaggca
     6541 cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct
     6601 ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga
     6661 gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta
     6721 taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc
     6781 ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc
     6841 tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta
     6901 ctttatattt atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga
     6961 agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg
     7021 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     7081 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     7141 cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt
     7201 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     7261 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     7321 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     7381 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     7441 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     7501 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     7561 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     7621 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaacgcg
     7681 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     7741 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     7801 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     7861 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     7921 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     7981 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     8041 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     8101 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     8161 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     8221 gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt
     8281 acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc
     8341 aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca
     8401 caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     8461 caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa
     8521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10441 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10501 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10561 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10621 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg
    10681 tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
    10741 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
    10801 tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct
    10861 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
    10921 cggcggtgga atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca
    10981 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
    11041 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
    11101 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
    11161 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
    11221 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
    11281 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtt tagttcctca
    11341 ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca acacgtgctg
    11401 atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc
    11461 aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc
    11521 ataaat
//
