LOCUS       sequence-432480-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression vector for 6xHis-TEV-hsDDB1∆B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, 
            Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, 
            Deb A, Choudhary A, Fischer ES, Liu DR
  TITLE     Continuous evolution of compact protein degradation tags regulated 
            by selective molecular glues.
  JOURNAL   Science. 2024 Mar 15;383(6688):eadk4422. doi: 
            10.1126/science.adk4422. Epub 2024 Mar 15.
  PUBMED    38484051
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-03-15"; volume: "383(6688):eadk4422. doi"; pages: " 
            10.1126/science.adk4422. Epub 2024 Mar 15"
COMMENT     Sequence Label: pSSRB069_pAC8-hsDDB1dB
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..102
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     27..44
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             126..143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             156..176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_recomb     2733..4090
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2749..4090)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4237..4692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4324..4343)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4534..4555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4709..4728)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4828..4850
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4888..4906)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4974..5078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5079..5147,5148..5939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5297..5316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6110..6698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6599..6618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6852..6869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     7261..8088
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             7261..7385
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(7423..8028)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(8092..8125)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    8315..8363
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8388..8407
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(8441..9118)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(8701..8720)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(9127..9236)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 agttgctgat atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
       61 tactgttttc gtaacagttt tgtaataaaa aaacctataa atacggatct accatgggca
      121 gcagccatca tcatcatcat cactccgcgg tcgacgagaa cctctacttc cagggaggcg
      181 gccgcatgtc gtacaactac gtggtaacgg cccagaagcc caccgccgtg aacggctgcg
      241 tgaccggaca ctttacttcg gccgaagact taaacctgtt gattgccaaa aacacgagat
      301 tagagatcta tgtggtcacc gccgaggggc ttcggcccgt caaagaggtg ggcatgtatg
      361 ggaagattgc ggtcatggag cttttcaggc ccaaggggga gagcaaggac ctgctgttta
      421 tcttgacagc gaagtacaat gcctgcatcc tggagtataa acagagtggc gagagcattg
      481 acatcattac gcgagcccat ggcaatgtcc aggaccgcat tggccgcccc tcagagaccg
      541 gcattattgg catcattgac cctgagtgcc ggatgattgg cctgcgtctc tatgatggcc
      601 ttttcaaggt tattccacta gatcgcgata ataaagaact caaggccttc aacatccgcc
      661 tggaggagct gcatgtcatt gatgtcaagt tcctatatgg ttgccaagca cctactattt
      721 gctttgtcta ccaggaccct caggggcggc acgtaaaaac ctatgaggtg tctctccgag
      781 aaaaggaatt caataagggc ccttggaaac aggaaaatgt cgaagctgaa gcttccatgg
      841 tgatcgcagt cccagagccc tttggggggg ccatcatcat tggacaggag tcaatcacct
      901 atcacaatgg tgacaaatac ctggctattg cccctcctat catcaagcaa agcacgattg
      961 tgtgccacaa tcgagtggac cctaatggct caagatacct gctgggagac atggaaggcc
     1021 ggctcttcat gctgcttttg gagaaggagg aacagatgga tggcaccgtc actctcaagg
     1081 atctccgtgt agaactcctt ggagagacct ctattgctga gtgcttgaca taccttgata
     1141 atggtgttgt gtttgtcggg tctcgcctgg gtgactccca gcttgtgaag ctcaacgttg
     1201 acagtaatga acaaggctcc tatgtagtgg ccatggaaac ctttaccaac ttaggaccca
     1261 ttgtcgatat gtgcgtggtg gacctggaga ggcaggggca ggggcagctg gtcacttgct
     1321 ctggggcttt caaggaaggt tctttgcgga tcatccggaa tggaattgga ggaaatggca
     1381 actcgggcga gatccagaag ctgcacattc gcacagttcc cctctatgag tctccaagga
     1441 agatctgcta ccaggaagtg tcccagtgtt tcggggtcct ctccagccgc attgaagtcc
     1501 aagacacgag tgggggcacg acagccttga ggcccagcgc tagcacccag gctctgtcca
     1561 gcagtgtaag ctccagcaag ctgttctcca gcagcactgc tcctcatgag acctcctttg
     1621 gagaagaggt ggaggtgcac aacctactta tcattgacca acacaccttt gaagtgcttc
     1681 atgcccacca gtttctgcag aatgaatatg ccctcagtct ggtttcctgc aagctgggca
     1741 aagaccccaa cacttacttc attgtgggca cagcaatggt gtatcctgaa gaggcagagc
     1801 ccaagcaggg tcgcattgtg gtctttcagt attcggatgg aaaactacag actgtggctg
     1861 aaaaggaagt gaaaggggcc gtgtactcta tggtggaatt taacgggaag ctgttagcca
     1921 gcatcaatag cacggtgcgg ctctatgagt ggacaacaga gaaggagctg cgcactgagt
     1981 gcaaccacta caacaacatc atggccctct acctgaagac caagggcgac ttcatcctgg
     2041 tgggcgacct tatgcgctca gtgctgctgc ttgcctacaa gcccatggaa ggaaactttg
     2101 aagagattgc tcgagacttt aatcccaact ggatgagtgc tgtggaaatc ttggatgatg
     2161 acaattttct gggggctgaa aatgccttta acttgtttgt gtgtcaaaag gatagcgctg
     2221 ccaccactga cgaggagcgg cagcacctcc aggaggttgg tcttttccac ctgggcgagt
     2281 ttgtcaatgt cttttgccac ggctctctgg taatgcagaa tctgggtgag acttccaccc
     2341 ccacacaagg ctcggtgctc ttcggcacgg tcaacggcat gatagggctg gtgacctcac
     2401 tgtcagagag ctggtacaac ctcctgctgg acatgcagaa tcgactcaat aaagtcatca
     2461 aaagtgtggg gaagatcgag cactccttct ggagatcctt tcacaccgag cggaagacag
     2521 aaccagccac aggtttcatc gacggtgact tgattgagag tttcctggat attagccgcc
     2581 ccaagatgca ggaggtggtg gcaaacctac agtatgacga tggcagcggt atgaagcgag
     2641 aggccactgc agacgacctc atcaaggttg tggaggagct aactcggatc cattagggta
     2701 cccccggacg gccgcttaat taattgatcc gggttattag tacatttatt aagcgctaga
     2761 ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt
     2821 ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct
     2881 gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa
     2941 gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa
     3001 acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg
     3061 taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc
     3121 gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat atttaacatc
     3181 gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt
     3241 tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac
     3301 gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg ggcgtttttg
     3361 ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag aaagcgatgg
     3421 tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg gtggagctga
     3481 tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg gaggcggagg
     3541 tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca acacagtcgg
     3601 cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc tgagacgagt
     3661 gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta aaggtgcagc
     3721 gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg atggtggtgg
     3781 tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg
     3841 tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc
     3901 tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg gcaattcaat
     3961 attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt cgctatcgtt
     4021 taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa gagattgtct
     4081 caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact acagcattgt
     4141 agtggcgaga cacttcgctg tcgtcgcctg atgcggtatt ttctccttac gcatctgtgc
     4201 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4441 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4561 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4681 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4741 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4861 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4921 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5101 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5161 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5221 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5281 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5341 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5401 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5461 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5521 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5581 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5641 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5701 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5761 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5821 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5881 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5941 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6001 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6061 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6241 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6721 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6781 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6901 ctctccccgc gcgttggccg attcattaat gcagaacggc tccgcccact attaatgaaa
     6961 ttaaaaattc caattttaaa aaacgcagca agagaaacat ttgtatgaaa gaatgcgtag
     7021 aaggaaagaa aaatgtcgtc gacatgctga acaacaagat taatatgcct ccgtgtataa
     7081 aaaaaatatt gaacgatttg aaagaaaaca atgtaccgcg cggcggtatg tacaggaaga
     7141 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
     7201 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
     7261 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
     7321 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
     7381 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
     7441 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
     7501 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
     7561 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
     7621 ctcctcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
     7681 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
     7741 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
     7801 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
     7861 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
     7921 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
     7981 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
     8041 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tataacttcg
     8101 tatagcatac attatacgaa gttatctggt tgaaacgtac ccgtagtggc tatggcaggg
     8161 cttgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg gggttggggt
     8221 ggggaaaagg aagaaacgcg ggcgtattgg tcccaatggg gtctcggtgg ggtatcgaca
     8281 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt
     8341 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     8401 tgtttcagtt agcctccccc atctcccgct attactcgag tcactgggag ccggagtggc
     8461 gggcctcggc gtgctcgtac tgctccacca cggtgtagtc ctcgttgtgg ttggtgatgt
     8521 ccagcttgga gtccacgtag tggttgccgg gcagctgcac gggcttcttg gccttgtaca
     8581 cggtcttgaa gtcgcaggtg tagtggccgc cgtccttcag cttcagggcg tgggagatct
     8641 cgcccttcag cacgccgtcc tgggggtaca gcttctcggt ggagggctcc cagccggcag
     8701 tcttcttctg cattacgggg ccgtcggcgg ggaagttcac gcccttgaac ttcaccttgt
     8761 agatgaaggt gccgtcctgc agggaggagt cctgctgcac ctccaccacg ccgccgtcct
     8821 cgaagttcat ggagcgctcc caggtgaagc cctcggggaa ggacagcttc atgtagtcgg
     8881 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta ctggaactgg ggggacagga
     8941 tgtcccaggc gaagggcagg gggccgccct tggtcacctg cagcttggcg gtctgggtgc
     9001 cctcgtaggg cttgccctcg ccctcgccct cgatctcgaa gtagtggccg ttcacggagc
     9061 cctccatgcg caccttgaac tgcatgaact ccttgatgac gtcctcggtg ttgtccatgg
     9121 tagagtgatt gtaaataaaa tgtaatttac agtatagtat tttaattaat atacaaatga
     9181 tttgataata attcttattt aactataata tattgtgttg ggttgaatta aaggtccgta
     9241 tactagtatc gattcgcgac ctactccgga atattaatag
//
