LOCUS       sequence-432483-        8842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, 
            Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, 
            Deb A, Choudhary A, Fischer ES, Liu DR
  TITLE     Continuous evolution of compact protein degradation tags regulated 
            by selective molecular glues.
  JOURNAL   Science. 2024 Mar 15;383(6688):eadk4422. doi: 
            10.1126/science.adk4422. Epub 2024 Mar 15.
  PUBMED    38484051
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-03-15"; volume: "383(6688):eadk4422. doi"; pages: " 
            10.1126/science.adk4422. Epub 2024 Mar 15"
COMMENT     Sequence Label: pSSRB274_pAC8-eGFP-hsCRBN
FEATURES             Location/Qualifiers
     source          1..8842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     759..776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     1168..1995
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1168..1292
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1330..1935)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(1999..2032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2222..2270
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2295..2314
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2348..3025)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(2608..2627)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(3034..3143)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3198..3289
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3214..3231
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3304..3327
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3340..3360
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3370..3372,3373..4083)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             4084..4107
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_recomb     5482..6839
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5498..6839)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      6986..7441
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7073..7092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7283..7304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7458..7477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7577..7599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7637..7655)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7723..7827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7828..7896,7897..8688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8046..8065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gaacggctcc gcccactatt aatgaaatta aaaattccaa ttttaaaaaa cgcagcaaga
      901 gaaacatttg tatgaaagaa tgcgtagaag gaaagaaaaa tgtcgtcgac atgctgaaca
      961 acaagattaa tatgcctccg tgtataaaaa aaatattgaa cgatttgaaa gaaaacaatg
     1021 taccgcgcgg cggtatgtac aggaagaggt ttatactaaa ctgttacatt gcaaacgtgg
     1081 tttcgtgtgc caagtgtgaa aaccgatgtt taatcaaggc tctgacgcat ttctacaacc
     1141 acgactccaa gtgtgtgggt gaagtcatgc atcttttaat caaatcccaa gatgtgtata
     1201 aaccaccaaa ctgccaaaaa atgaaaactg tcgacaagct ctgtccgttt gctggcaact
     1261 gcaagggtct caatcctatt tgtaattatt gaataataaa acaattataa atgtcaaatt
     1321 tgttttttat taacgataca aaccaaacgc aacaagaaca tttgtagtat tatctataat
     1381 tgaaaacgcg tagttataat cgctgaggta atatttaaaa tcattttcaa atgattcaca
     1441 gttaatttgc gacaatataa ttttattttc acataaacta gacgccttgt cgtcttcttc
     1501 ttcgtattcc ttctcttttt catttttctc ctcataaaaa ttaacatagt tattatcgta
     1561 tccatatatg tatctatcgt atagagtaaa ttttttgttg tcataaatat atatgtcttt
     1621 tttaatgggg tgtatagtac cgctgcgcat agtttttctg taatttacaa cagtgctatt
     1681 ttctggtagt tcttcggagt gtgttgcttt aattattaaa tttatataat caatgaattt
     1741 gggatcgtcg gttttgtaca atatgttgcc ggcatagtac gcagcttctt ctagttcaat
     1801 tacaccattt tttagcagca ccggattaac ataactttcc aaaatgttgt acgaaccgtt
     1861 aaacaaaaac agttcacctc ccttttctat actattgtct gcgagcagtt gtttgttgtt
     1921 aaaaataaca gccattgtaa tgagacgcac aaactaatat cacaaactgg aaatgtctat
     1981 caatatatag ttgctgatat aacttcgtat agcatacatt atacgaagtt atctggttga
     2041 aacgtacccg tagtggctat ggcagggctt gccgccccga cgttggctgc gagccctggg
     2101 ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc
     2161 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     2221 tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     2281 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgctatt
     2341 actcgagtca ctgggagccg gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg
     2401 tgtagtcctc gttgtggttg gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca
     2461 gctgcacggg cttcttggcc ttgtacacgg tcttgaagtc gcaggtgtag tggccgccgt
     2521 ccttcagctt cagggcgtgg gagatctcgc ccttcagcac gccgtcctgg gggtacagct
     2581 tctcggtgga gggctcccag ccggcagtct tcttctgcat tacggggccg tcggcgggga
     2641 agttcacgcc cttgaacttc accttgtaga tgaaggtgcc gtcctgcagg gaggagtcct
     2701 gctgcacctc caccacgccg ccgtcctcga agttcatgga gcgctcccag gtgaagccct
     2761 cggggaagga cagcttcatg tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     2821 agccgtactg gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg
     2881 tcacctgcag cttggcggtc tgggtgccct cgtagggctt gccctcgccc tcgccctcga
     2941 tctcgaagta gtggccgttc acggagccct ccatgcgcac cttgaactgc atgaactcct
     3001 tgatgacgtc ctcggtgttg tccatggtag agtgattgta aataaaatgt aatttacagt
     3061 atagtatttt aattaatata caaatgattt gataataatt cttatttaac tataatatat
     3121 tgtgttgggt tgaattaaag gtccgtatac tagtatcgat tcgcgaccta ctccggaata
     3181 ttaatagagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     3241 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatctacc
     3301 atggactaca aagacgatga cgacaagtcc gcggtcgacg agaacctcta cttccaggga
     3361 ggcggccgcg tatcaaaggg agaagagtta ttcactggcg tagtgcctat cctagtcgag
     3421 ttagatggcg acgtcaatgg ccataagttc tccgtctccg gtgaaggaga aggagacgcc
     3481 acgtacggaa aattgacact caagttcata tgcaccaccg gtaaactacc cgtaccatgg
     3541 cctacgcttg tcaccacctt gacctatggt gtgcaatgct tctcaaggta tcctgaccac
     3601 atgaagcaac atgacttctt taaatccgcg atgcccgagg gctacgttca ggaacgaact
     3661 attttcttta aagatgacgg taactacaaa acgagggcag aagtcaagtt cgaaggagat
     3721 actctggtca accgcattga attgaaaggc attgacttta aggaggacgg taacatcttg
     3781 ggacataaac tcgagtacaa ttacaacagt cacaacgtgt acataatggc tgataaacaa
     3841 aagaacggta taaaagtgaa cttcaagata agacacaata tagaggacgg cagcgtgcaa
     3901 ctggctgatc actaccaaca gaatactccg atcggagacg gcccagtgtt gttgcctgac
     3961 aatcactatt tgagtactca gtccgctctc tcaaaggacc caaacgagaa gcgtgaccac
     4021 atggtcctac tcgagttcgt tactgcagct ggaataactt taggtatgga tgagttgtac
     4081 aagctcgagg tcttgttcca aggaccgggc ggatccatgg ccggtgaggg agaccagcag
     4141 gacgcagcac acaacatggg caatcacctg ccattgcttc ctgccgagtc tgaggaagaa
     4201 gacgagatgg aggtcgaaga ccaagactca aaggaagcta agaaacctaa catcatcaat
     4261 ttcgatacca gcttgcctac ctcccacaca tacttgggcg ctgacatgga agaatttcat
     4321 ggacgtacct tacacgacga tgattcctgc caggtgatcc ctgtgttacc tcaagtcatg
     4381 atgatcttaa tcccgggaca aaccctgcca cttcagctgt tccacccaca agaagtgtcc
     4441 atggtccgta acctcataca gaaggaccgt accttcgcag tattggccta ctcgaacgtc
     4501 caagaacgtg aagcgcagtt cggtacgacc gccgagatct acgcttatcg cgaggagcaa
     4561 gacttcggta tcgagattgt caaagtgaaa gccataggca gacagcgctt caaggtactc
     4621 gagctgcgta cacagtcaga tggaatccag caagctaaag ttcagatctt gcctgagtgc
     4681 gtcttgccga gcacgatgtc agccgtccaa ctggaaagct tgaataagtg ccaaattttc
     4741 ccttccaaac ccgtatccag agaggaccaa tgctcttaca agtggtggca gaagtaccag
     4801 aagaggaaat tccactgtgc aaaccttact tcatggccca ggtggctgta ttcgctgtac
     4861 gacgccgaga ccttgatgga tcgaattaaa aagcagctgc gagagtggga cgagaacttg
     4921 aaggatgact ccctgccctc caatccgata gacttctcct accgcgtagc ggcctgttta
     4981 ccaatcgacg acgttctacg catccaactg ctgaaaatag gctcagcaat ccagaggctg
     5041 cgatgcgagc tggacattat gaacaagtgc acgtccttat gctgcaaaca atgtcaggag
     5101 actgagataa cgactaagaa cgagattttc tcgctatctt tgtgcggacc catggcagct
     5161 tacgtaaacc cacatggtta cgtccatgag actcttactg tgtacaaagc ttgtaacctt
     5221 aacctcatag gaagaccatc gactgagcac tcctggtttc caggttatgc gtggacagtt
     5281 gctcagtgca agatctgcgc cagccacatc ggttggaagt ttaccgcaac aaaaaaggac
     5341 atgtctccac agaagttctg gggcctcacc aggagcgctc tcttgccaac catccccgat
     5401 actgaggacg aaatctcccc agacaaagta attctgtgcc tgtaaggtac ccccggacgg
     5461 ccgcttaatt aattgatccg ggttattagt acatttatta agcgctagat tctgtgcgtt
     5521 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
     5581 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
     5641 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
     5701 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
     5761 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
     5821 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca
     5881 attgactcga cgtaaacacg ttaaataaag cttggacata tttaacatcg ggcgtgttag
     5941 ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag
     6001 acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca
     6061 aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca
     6121 atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg
     6181 gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta
     6241 ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg
     6301 atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta
     6361 ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt
     6421 cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt
     6481 ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag
     6541 gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt
     6601 ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa
     6661 cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg
     6721 aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga
     6781 tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcggat
     6841 cgatcccgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
     6901 acttcgctgt cgtcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6961 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7021 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7081 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7141 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7201 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7261 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7321 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7381 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     7441 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7501 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7561 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7621 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7681 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7801 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7861 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7921 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7981 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8041 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8101 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8161 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8221 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8281 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8341 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8401 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8461 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8521 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8581 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8641 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8701 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8761 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8821 tgagcgtcag accccgtaga aa
//
