LOCUS       sequence-432516-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable, inducible expression in lysosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, 
            Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ,
            Huttenhain R
  TITLE     An automated proximity proteomics pipeline for subcellular proteome 
            and protein interaction mapping.
  JOURNAL   bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 
            10.1101/2023.04.11.536358. Preprint.
  PUBMED    37090610
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. 
            Preprint."
COMMENT     Sequence Label: LAMTOR1-APEX2
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    76..191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(454..498)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    683..916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1413..1538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1585..2218
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2386..2520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2568..2672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2673..2741,2742..3533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2891..2910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3704..4292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4193..4212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4446..4463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4580..4601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4616..4621,4622..4639,4640..4646)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4654..4670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4659..4681
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4678..4694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4678..4694
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4822..5455
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5662..6250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5768..5779
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6177..6197
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6264..6863)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6347..6367)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6844..6863
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6872..7201)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6886..7021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7212..7958)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7977..8487)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8040..8058)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8504..8871
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8512..8530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8548..8566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8584..8602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8620..8638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8656..8674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8692..8710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8728..8746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8878..8887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9031..9033,9034..9036,9037..9747)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9031..9033,9034..9036,9037..9747)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9076..9097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9337..9356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9684..9705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      9760..9769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9769..9792
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9793..10539
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             10546..10572
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    join(10759..10861,1..32)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttgtaaccat tataagctgc aataaacaag ttaagtcgat aaactggatc tctgctgtcc
       61 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
      121 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
      181 tttcttttaa aggcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc
      241 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
      301 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
      361 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
      421 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
      481 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
      541 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
      601 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
      661 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
      721 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
      781 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
      841 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
      901 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
      961 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     1021 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc
     1081 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     1141 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     1201 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     1261 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     1321 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     1381 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     1441 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     1501 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     1561 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     1621 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     1681 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     1741 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     1801 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     1861 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     1921 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     1981 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     2041 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     2101 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     2161 tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag cccttccaac
     2221 tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa ataattcaga
     2281 ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa tagggtatgg
     2341 ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata
     2401 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2461 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2521 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2581 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2641 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2701 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2761 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2821 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2881 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2941 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3001 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3061 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3181 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3241 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3301 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3541 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4501 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4561 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4621 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4681 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt aaacgctagc
     4741 aatgtcaagg cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     4801 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     4861 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     4921 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     4981 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     5041 agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa
     5101 agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg cagctctcgg
     5161 gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc tctctccggg
     5221 tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc tttattggcc
     5281 tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     5341 tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg
     5401 tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc ttccagtccc
     5461 ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     5521 ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc tagccaggca
     5581 caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt ttctaggtct
     5641 cgactgcaga attaattcca ggcggggagg cggcccaaag ggagatccga ctcgtctgag
     5701 ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt
     5761 ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg
     5821 caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc acggaattgt
     5881 cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg
     5941 ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa
     6001 tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga
     6061 caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa
     6121 tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata
     6181 gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca
     6241 gaggttgatt gttccagacg cgttcaggca ccgggcttgc gggtcatgca ccaggtgcgc
     6301 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
     6361 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
     6421 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg
     6481 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
     6541 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
     6601 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg
     6661 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
     6721 tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt ggcgcgtggc ggggtagtcg
     6781 gcgaacgcgg cggcgagggt gcgtacggcc cgggggacgt cgtcgcgggt ggcgaggcgc
     6841 accgtgggct tgtactcggt catggtcgcg ttttgcaaaa gcctaggcct ccaaaaaagc
     6901 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     6961 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     7021 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     7081 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     7141 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     7201 cattcgcgcg tttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     7261 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     7321 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     7381 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     7441 gcttgtttca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     7501 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     7561 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     7621 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
     7681 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     7741 tcgccttcca gggggcagga gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     7801 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     7861 ttttgagcga gtttccttgt cgtcaggcct tcgataccga ctccattgag taattccaga
     7921 gcagagttta tgactttgct cttgtccagt ctagacatgg taattcgatg atccccctgg
     7981 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     8041 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     8101 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     8161 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     8221 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     8281 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     8341 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     8401 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     8461 ccttggaaaa ggcgcaaccc caaccccgtg gaattatcac ctcgagttta ctccctatca
     8521 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
     8581 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
     8641 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
     8701 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagctttg
     8761 cttatgtaaa ccagggcgcc tataaaagag tgctgatttt ttgagtaaac ttcaattcca
     8821 caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa agaattcgcc
     8881 accatggggt gctgctacag cagcgagaac gaggactcgg accaggaccg agaggagcgg
     8941 aagctgctgc tggaccctag cagcccccct accaaagctc tcaatggagc cgagcccaac
     9001 taccctggtg gatctggagg ttctggtgga atggtgagca agggcgagga gctgttcacc
     9061 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     9121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     9181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     9241 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     9301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     9361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     9421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     9481 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     9541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     9601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     9661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     9721 actctcggca tggacgagct gtacaagtcc ggagcggccg ccaccatgga ctacaaggat
     9781 gacgacgata agggaaagtc ttacccaact gtgagtgctg attaccagga cgccgttgag
     9841 aaggcgaaga agaagctcag aggcttcatc gctgagaaga gatgcgctcc tctaatgctc
     9901 cgtttggcat tccactctgc tggaaccttt gacaagggca cgaagaccgg tggacccttc
     9961 ggaaccatca agcaccctgc cgaactggct cacagcgcta acaacggtct tgacatcgct
    10021 gttaggcttt tggagccact caaggcggag ttccctattt tgagctacgc cgatttctac
    10081 cagttggctg gcgttgttgc cgttgaggtc acgggtggac ctaaggttcc attccaccct
    10141 ggaagagagg acaagcctga gccaccacca gagggtcgct tgcccgatcc cactaagggt
    10201 tctgaccatt tgagagatgt gtttggcaaa gctatggggc ttactgacca agatatcgtt
    10261 gctctatctg ggggtcacac tattggagct gcacacaagg agcgttctgg atttgagggt
    10321 ccctggacct ctaatcctct tattttcgac aactcatact tcacggagtt gttgagtggt
    10381 gagaaggaag gtctccttca gctaccttct gacaaggctc ttttgtctga ccctgtattc
    10441 cgccctctcg ttgacaaata tgcagcggac gaagatgcct tctttgctga ttacgctgag
    10501 gctcaccaaa agctttccga gcttgggttt gctgatgccc tgcagctgcc tcccctggag
    10561 cgcctgaccc tggactaagg atccagacca cctcccctgc gagctaagct ggacagccaa
    10621 tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact
    10681 gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc
    10741 agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc
    10801 aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta
    10861 t
//
