LOCUS       sequence-432517-       10975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable, inducible expression in Golgi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, 
            Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ,
            Huttenhain R
  TITLE     An automated proximity proteomics pipeline for subcellular proteome 
            and protein interaction mapping.
  JOURNAL   bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 
            10.1101/2023.04.11.536358. Preprint.
  PUBMED    37090610
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. 
            Preprint."
COMMENT     Sequence Label: GALT-APEX2
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(511..555)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    740..973
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1470..1595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1642..2275
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2443..2577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2625..2729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2730..2798,2799..3590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2948..2967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3761..4349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4250..4269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4503..4520
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4637..4658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4673..4678,4679..4696,4697..4703)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4711..4727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4716..4738
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4735..4751
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4735..4751
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4879..5512
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5719..6307
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5825..5836
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6234..6254
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6321..6920)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6404..6424)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6901..6920
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6929..7258)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6943..7078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7269..8015)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(8034..8544)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8097..8115)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8561..8928
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8569..8587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8605..8623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8641..8659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8677..8695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8713..8731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8749..8767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8785..8803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8938..8947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8944..9693
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     CDS             9700..9726
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      10015..10024
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10021..10023,10024..10026,10027..10740)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10021..10023,10024..10026,10027..10737)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10066..10087)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10327..10346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10674..10695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    join(10930..10975,1..89)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt gccttatttg
       61 taaccattat aagctgcaat aaacaagtta agtcgataaa ctggatctct gctgtccctg
      121 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
      181 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
      241 cttttaaagg cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct
      301 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
      361 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
      421 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
      481 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
      541 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
      601 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
      661 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
      721 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
      781 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
      841 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
      901 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
      961 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     1021 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     1081 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccggc cgcttgctgt
     1141 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     1201 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     1261 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     1321 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
     1381 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     1441 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     1501 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     1561 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     1621 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     1681 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1741 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1801 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     1861 agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa
     1921 agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg cagctctcgg
     1981 gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc tctctccggg
     2041 tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc tttattggcc
     2101 tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     2161 tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg
     2221 tagatccaca gatcaaggat atcttgtctt ctttgggagt gaattagccc ttccaactac
     2281 taagtttgta gtacatattt aacaaataca atttctttaa aatgaaaata attcagagga
     2341 atcacaggtt tagagtaaat gaaaccacag gtaattggca gtggtaatag ggtatggggt
     2401 gggaagtttg ggatgatttt ggttagcttg agttatccag ttgatccaga catgataaga
     2461 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2521 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttgat
     2581 ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4381 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4441 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4501 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4561 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4621 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4681 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4741 acagctatga ccatgattac gccaagctct agctagaggt cgacggtaaa cgctagcaat
     4801 gtcaaggcct ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa
     4861 gatgacatga actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     4921 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     4981 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     5041 ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg
     5101 gctcgccact ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt
     5161 cccttgtagc aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc
     5221 acgtgatgaa atgctaggcg gctgtcaaac ctccactcta acacttctct ctccgggtca
     5281 tccatcccat gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct
     5341 tctaccttat ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     5401 ctgacccctg gccctggtgt gtagttctgc taatcaggga agtagccttg tgtgtggtag
     5461 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccctc
     5521 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     5581 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     5641 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     5701 ctgcagaatt aattccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     5761 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     5821 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     5881 cacaggcgag cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag
     5941 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     6001 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     6061 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     6121 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     6181 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     6241 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     6301 gttgattgtt ccagacgcgt tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     6361 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     6421 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     6481 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     6541 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     6601 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     6661 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     6721 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     6781 gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg
     6841 aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc
     6901 gtgggcttgt actcggtcat ggtcgcgttt tgcaaaagcc taggcctcca aaaaagcctc
     6961 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     7021 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     7081 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     7141 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     7201 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     7261 tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
     7321 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
     7381 agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
     7441 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
     7501 tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
     7561 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
     7621 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
     7681 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
     7741 gagaggagag cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
     7801 ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
     7861 agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
     7921 tgagcgagtt tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagca
     7981 gagtttatga ctttgctctt gtccagtcta gacatggtaa ttcgatgatc cccctgggga
     8041 gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg
     8101 cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc
     8161 tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg
     8221 gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg
     8281 gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa
     8341 ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg
     8401 aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg
     8461 ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct
     8521 tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc gagtttactc cctatcagtg
     8581 atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac
     8641 tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta
     8701 tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg
     8761 atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata agctttgctt
     8821 atgtaaacca gggcgcctat aaaagagtgc tgattttttg agtaaacttc aattccacaa
     8881 cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaaga attcgccgcc
     8941 accatgggaa agtcttaccc aactgtgagt gctgattacc aggacgccgt tgagaaggcg
     9001 aagaagaagc tcagaggctt catcgctgag aagagatgcg ctcctctaat gctccgtttg
     9061 gcattccact ctgctggaac ctttgacaag ggcacgaaga ccggtggacc cttcggaacc
     9121 atcaagcacc ctgccgaact ggctcacagc gctaacaacg gtcttgacat cgctgttagg
     9181 cttttggagc cactcaaggc ggagttccct attttgagct acgccgattt ctaccagttg
     9241 gctggcgttg ttgccgttga ggtcacgggt ggacctaagg ttccattcca ccctggaaga
     9301 gaggacaagc ctgagccacc accagagggt cgcttgcccg atcccactaa gggttctgac
     9361 catttgagag atgtgtttgg caaagctatg gggcttactg accaagatat cgttgctcta
     9421 tctgggggtc acactattgg agctgcacac aaggagcgtt ctggatttga gggtccctgg
     9481 acctctaatc ctcttatttt cgacaactca tacttcacgg agttgttgag tggtgagaag
     9541 gaaggtctcc ttcagctacc ttctgacaag gctcttttgt ctgaccctgt attccgccct
     9601 ctcgttgaca aatatgcagc ggacgaagat gccttctttg ctgattacgc tgaggctcac
     9661 caaaagcttt ccgagcttgg gtttgctgat gccctgcagc tgcctcccct ggagcgcctg
     9721 accctggacg tcgagctcaa gcttgccgcc accggaaggc ttcgggagcc gctcctgagc
     9781 ggcagcgccg cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc
     9841 tgcgctctgc accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc
     9901 ctgccccaac tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc
     9961 atcgggcagt cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc
    10021 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
    10081 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
    10141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
    10201 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
    10261 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
    10321 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
    10381 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
    10441 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
    10501 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
    10561 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
    10621 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
    10681 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
    10741 agcggccgcg gatccagacc acctcccctg cgagctaagc tggacagcca atgacgggta
    10801 agagagtgac atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc
    10861 aaagacgggt aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga
    10921 gggatttgag atccagacat gataagatac attgatgagt ttggacaaac caaaa
//
