LOCUS       sequence-432521-       10804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable, inducible expression in plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, 
            Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ,
            Huttenhain R
  TITLE     An automated proximity proteomics pipeline for subcellular proteome 
            and protein interaction mapping.
  JOURNAL   bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 
            10.1101/2023.04.11.536358. Preprint.
  PUBMED    37090610
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. 
            Preprint."
COMMENT     Sequence Label: PM-APEX2
FEATURES             Location/Qualifiers
     source          1..10804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             141..167
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    354..488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    532..647
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(910..954)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1139..1372
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1869..1994
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2041..2674
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2842..2976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3024..3128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3129..3197,3198..3989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3347..3366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4160..4748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4649..4668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4902..4919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5036..5057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5072..5077,5078..5095,5096..5102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5110..5126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5115..5137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5134..5150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5134..5150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             5278..5911
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6118..6706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6224..6235
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6633..6653
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6720..7319)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6803..6823)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7300..7319
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7328..7657)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7342..7477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7668..8414)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(8433..8943)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8496..8514)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8960..9327
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8968..8986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9004..9022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9040..9058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9076..9094
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9112..9130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9148..9166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9184..9202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(9403..9405,9406..10116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9403..9405,9406..10116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9445..9466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9706..9725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10053..10074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      10159..10168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10168..10191
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(10192..10804,1..134)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 cttctgacaa ggctcttttg tctgaccctg tattccgccc tctcgttgac aaatatgcag
       61 cggacgaaga tgccttcttt gctgattacg ctgaggctca ccaaaagctt tccgagcttg
      121 ggtttgctga tgccctgcag ctgcctcccc tggagcgcct gaccctggac taaggatcca
      181 gaccacctcc cctgcgagct aagctggaca gccaatgacg ggtaagagag tgacattttt
      241 cactaaccta agacaggagg gccgtcagag ctactgccta atccaaagac gggtaaaagt
      301 gataaaaatg tatcactcca acctaagaca ggcgcagctt ccgagggatt tgagatccag
      361 acatgataag atacattgat gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat
      421 gccttatttg tgaaatttgt gatgctattg ccttatttgt aaccattata agctgcaata
      481 aacaagttaa gtcgataaac tggatctctg ctgtccctgt aataaacccg aaaattttga
      541 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
      601 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaggc gataccgtcg
      661 agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
      721 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
      781 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
      841 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
      901 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
      961 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
     1021 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
     1081 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
     1141 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
     1201 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
     1261 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
     1321 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
     1381 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     1441 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     1501 tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt acttttgttt
     1561 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     1621 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     1681 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     1741 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     1801 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     1861 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     1921 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     1981 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     2041 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     2101 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     2161 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     2221 cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca ctccccagtc
     2281 ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta gcaagctcga
     2341 tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg aaatgctagg
     2401 cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc atgcaggctc
     2461 acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt atctggctca
     2521 actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc tggccctggt
     2581 gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata
     2641 tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag tacatattta
     2701 acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt agagtaaatg
     2761 aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg gatgattttg
     2821 gttagcttga gttatccagt tgatccagac atgataagat acattgatga gtttggacaa
     2881 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2941 ttatttgtaa ccattataag ctgcaataaa caagttgatc tcccgatccg tcgacgtcag
     3001 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3061 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5161 ccaagctcta gctagaggtc gacggtaaac gctagcaatg tcaaggcctc tcactctctg
     5221 atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa ctactactgc
     5281 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     5341 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     5401 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     5461 tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg
     5521 cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat
     5581 cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg
     5641 ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca
     5701 gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact
     5761 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg
     5821 tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
     5881 tgtcttcgtt gggagtgaat tagcccttcc agtcccctct tttcttttaa aaagtggcta
     5941 agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt gactggaaaa
     6001 cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt agctgctgta
     6061 ttgctacttg tgattgctcc atgtttttct aggtctcgac tgcagaatta attccaggcg
     6121 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     6181 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     6241 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccatggaa
     6301 aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     6361 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     6421 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     6481 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     6541 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     6601 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     6661 gaataccagt caatctttca caaattttgt aatccagagg ttgattgttc cagacgcgtt
     6721 caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg
     6781 gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc
     6841 aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg
     6901 cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc
     6961 tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa
     7021 ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc
     7081 tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac cttgccgatg
     7141 tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg
     7201 tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt
     7261 acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg
     7321 gtcgcgtttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     7381 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     7441 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     7501 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     7561 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7621 gggagcctgg ggactttcca caccctaact gacacacatt cgcgcgttta cccggggagc
     7681 atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca
     7741 agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg
     7801 ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag
     7861 gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtttcagaag tgggggcata
     7921 gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat
     7981 acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag
     8041 aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt
     8101 gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggtat
     8161 gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg gcaggagtgg
     8221 gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc
     8281 acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc
     8341 aggccttcga taccgactcc attgagtaat tccagagcag agtttatgac tttgctcttg
     8401 tccagtctag acatggtaat tcgatgatcc ccctggggag agaggtcggt gattcggtca
     8461 acgagggagc cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg
     8521 ggcaggaaca gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg
     8581 ccgctgctct cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg
     8641 cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct
     8701 tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga
     8761 gcaggaagcg tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga
     8821 acggacgtga agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc
     8881 ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac
     8941 cccgtggaat tatcacctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt
     9001 tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac
     9061 gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca
     9121 gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt
     9181 tactccctat cagtgataga gaacgtataa gctttgctta tgtaaaccag ggcgcctata
     9241 aaagagtgct gattttttga gtaaacttca attccacaac acttttgtct tataccaact
     9301 ttccgtacca cttcctaccc tcgtaaagaa ttcaccatgg gatgtataaa atcaaaaggg
     9361 aaagacagcg cggatagtgc tggtagtgct ggtagtgctg gtgtgagcaa gggcgaggag
     9421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     9481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     9541 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     9601 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     9661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     9721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     9781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     9841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     9901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     9961 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
    10021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
    10081 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagaac tcgagctcaa
    10141 gcttcgaatt ctgcggccgc caccatggac tacaaggatg acgacgataa gggaaagtct
    10201 tacccaactg tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga
    10261 ggcttcatcg ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct
    10321 ggaacctttg acaagggcac gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc
    10381 gaactggctc acagcgctaa caacggtctt gacatcgctg ttaggctttt ggagccactc
    10441 aaggcggagt tccctatttt gagctacgcc gatttctacc agttggctgg cgttgttgcc
    10501 gttgaggtca cgggtggacc taaggttcca ttccaccctg gaagagagga caagcctgag
    10561 ccaccaccag agggtcgctt gcccgatccc actaagggtt ctgaccattt gagagatgtg
    10621 tttggcaaag ctatggggct tactgaccaa gatatcgttg ctctatctgg gggtcacact
    10681 attggagctg cacacaagga gcgttctgga tttgagggtc cctggacctc taatcctctt
    10741 attttcgaca actcatactt cacggagttg ttgagtggtg agaaggaagg tctccttcag
    10801 ctac
//
