LOCUS       sequence-432599-        7257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human FLVCR1/FLVCR1a cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Kenny TC, Khan A, Son Y, Yue L, Heissel S, Sharma A, Pasolli HA, Liu
            Y, Gamazon ER, Alwaseem H, Hite RK, Birsoy K
  TITLE     Integrative genetic analysis identifies FLVCR1 as a plasma-membrane 
            choline transporter in mammals.
  JOURNAL   Cell Metab. 2023 Jun 6;35(6):1057-1071.e12. doi: 
            10.1016/j.cmet.2023.04.003. Epub 2023 Apr 25.
  PUBMED    37100056
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2023.04.003"; journalName: "Cell Metab"; date: 
            "2023-06-6- 6"; volume: "35"; issue: "6"; pages: "1057-1071.e12"
COMMENT     Sequence Label: pMXS-IRES-Blast FLVCR1/FLVCR1a
FEATURES             Location/Qualifiers
     source          1..7257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(3609..4183,4184..4186,4187..4195)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3776..3793)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4003..4022
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4196..4591
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4762..5231
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5361..5378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5532..6120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5612..5631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6284..7075,7076..7144))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6907..6926
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7145..7249)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaatgg
     1921 cccgccccga tgacgaagag ggagctgccg tagctcctgg ccatccactg gccaaggggt
     1981 atctgcctct cccacggggt gcccctgtgg gaaaagaatc cgtcgaactt caaaatggcc
     2041 ctaaggccgg aacttttcct gttaacggcg cgcctcgtga ttctctggca gctgctagcg
     2101 gtgtattggg tggaccacaa accccacttg cacccgaaga agaaacacaa gccagattgc
     2161 tgccagccgg cgcgggcgcc gaaacccccg gcgccgaatc atcaccactt cctctgacag
     2221 ccctgtctcc tagacgattt gtcgtcctgc tcatatttag tctttactct ctcgtgaacg
     2281 cattccaatg gatacagtat tcaataatct caaatgtgtt tgaaggattt tatggcgtga
     2341 ctctgttgca tatagattgg cttagcatgg tctatatgct cgcttatgtt cctctgatat
     2401 ttccagctac atggcttctc gatactaggg gtctccgcct tacagcactc ctcgggtcag
     2461 gtctgaattg tctcggagca tggataaaat gtggatccgt ccaacaacac ctgttttggg
     2521 tgacgatgct cgggcaatgt ctttgttcag ttgcacaagt ctttattctc gggctgccat
     2581 caagaattgc ttctgtctgg ttcggcccta aggaagtaag cacggcctgc gcaacggcag
     2641 tccttgggaa ccaacttggc accgctgtcg ggttcctgct gccacccgtg ctggtgccta
     2701 atacccaaaa cgatacgaac ctgctcgcct gcaacatttc cactatgttc tacggcacct
     2761 ctgcagtggc gactctcctg ttcatactga ccgcgatcgc gttcaaggag aagccgcgct
     2821 acccaccgtc acaagcccag gctgccttgc aggattcacc acccgaggaa tatagctaca
     2881 agaagagcat caggaatttg ttcaagaata tcccgttcgt gctgttgctg attacctacg
     2941 gcattatgac aggggcattt tatagtgtgt caactctcct caatcaaatg attctcacat
     3001 attatgaagg tgaggaagtg aacgcaggca gaatcggcct gacccttgtg gtcgccggca
     3061 tggtaggtag catcctgtgc ggtttgtggc tcgactacac caagacgtat aagcaaacga
     3121 cccttattgt ctacatactg tccttcatcg gcatggtcat tttcaccttt actctggatc
     3181 tgaggtacat cattattgtc ttcgtgaccg gtggcgtcct ggggttcttt atgacaggct
     3241 atctgcccct ggggttcgag ttcgccgtcg agattacata tcccgagtca gaggggacat
     3301 cttccgggct gctgaacgca agcgcccaaa ttttcggtat cctgtttacc ctggcccagg
     3361 ggaaattgac ctctgattac ggaccgaaag ctggtaatat cttcctttgc gtgtggatgt
     3421 tcattgggat tatcctgacg gccctgatta aatccgacct tcggcggcat aatatcaaca
     3481 ttgggatcac caacgtcgac gtcaaggcca tccccgccga tagcccgact gatcaggagc
     3541 ctaagactgt gatgctgagt aaacaatctg agagtgctat ctaggctacg taaattccgc
     3601 cccccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     3661 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3721 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3781 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3841 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3901 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3961 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     4021 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     4081 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     4141 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     4201 tcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     4261 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     4321 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     4381 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     4441 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     4501 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     4561 gctgccctct ggttatgtgt gggagggcta agtcgacgat aaaataaaag attttattta
     4621 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     4681 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     4741 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     4801 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     4861 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     4921 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     4981 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     5041 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     5101 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     5161 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     5221 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
     5281 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5341 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc
     6241 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6301 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6361 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6421 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6481 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6541 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6601 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6661 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6721 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6781 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6841 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6901 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6961 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7021 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7081 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7141 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7201 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttc
//
