LOCUS       sequence-43260-e       13264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 13264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-09-27"; volume: "2:483. doi"; pages: " 
            10.1038/ncomms1494"
COMMENT     Sequence Label: pBbA5c-MevT-MBIS
FEATURES             Location/Qualifiers
     source          1..13264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(2309..2320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5233..5244
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8851..8862
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      11276..11347
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11363..11390
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      11429..11500
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11516..11543
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(11571..11588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11705..12250)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(11785..11804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(12364..12458)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(12479..13138)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     13053..13072
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(13139..13241)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
     1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
     1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
     1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
     1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
     1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
     2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
     2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
     2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
     2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
     2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
     2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
     2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
     2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
     2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
     2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
     2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
     2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
     2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct tgaattaagg aggacagcta
     2821 aatgaaactc tcaactaaac tttgttggtg tggtattaaa ggaagactta ggccgcaaaa
     2881 gcaacaacaa ttacacaata caaacttgca aatgactgaa ctaaaaaaac aaaagaccgc
     2941 tgaacaaaaa accagacctc aaaatgtcgg tattaaaggt atccaaattt acatcccaac
     3001 tcaatgtgtc aaccaatctg agctagagaa atttgatggc gtttctcaag gtaaatacac
     3061 aattggtctg ggccaaacca acatgtcttt tgtcaatgac agagaagata tctactcgat
     3121 gtccctaact gttttgtcta agttgatcaa gagttacaac atcgacacca acaaaattgg
     3181 tagattagaa gtcggtactg aaactctgat tgacaagtcc aagtctgtca agtctgtctt
     3241 gatgcaattg tttggtgaaa acactgacgt cgaaggtatt gacacgctta atgcctgtta
     3301 cggtggtacc aacgcgttgt tcaactcttt gaactggatt gaatctaacg catgggatgg
     3361 tagagacgcc attgtagttt gcggtgatat tgccatctac gataagggtg ccgcaagacc
     3421 aaccggtggt gccggtactg ttgctatgtg gatcggtcct gatgctccaa ttgtatttga
     3481 ctctgtaaga gcttcttaca tggaacacgc ctacgatttt tacaagccag atttcaccag
     3541 cgaatatcct tacgtcgatg gtcatttttc attaacttgt tacgtcaagg ctcttgatca
     3601 agtttacaag agttattcca agaaggctat ttctaaaggg ttggttagcg atcccgctgg
     3661 ttcggatgct ttgaacgttt tgaaatattt cgactacaac gttttccatg ttccaacctg
     3721 taaattggtc acaaaatcat acggtagatt actatataac gatttcagag ccaatcctca
     3781 attgttccca gaagttgacg ccgaattagc tactcgcgat tatgacgaat ctttaaccga
     3841 taagaacatt gaaaaaactt ttgttaatgt tgctaagcca ttccacaaag agagagttgc
     3901 ccaatctttg attgttccaa caaacacagg taacatgtac accgcatctg tttatgccgc
     3961 ctttgcatct ctattaaact atgttggatc tgacgactta caaggcaagc gtgttggttt
     4021 attttcttac ggttccggtt tagctgcatc tctatattct tgcaaaattg ttggtgacgt
     4081 ccaacatatt atcaaggaat tagatattac taacaaatta gccaagagaa tcaccgaaac
     4141 tccaaaggat tacgaagctg ccatcgaatt gagagaaaat gcccatttga agaagaactt
     4201 caaacctcaa ggttccattg agcatttgca aagtggtgtt tactacttga ccaacatcga
     4261 tgacaaattt agaagatcct acgatgttaa aaaatgagga tctaaaataa ggaggattac
     4321 actatggttt taaccaataa aacagtcatt tctggatcga aagtcaaaag tttatcatct
     4381 gcgcaatcga gctcatcagg accttcatca tctagtgagg aagatgattc ccgcgatatt
     4441 gaaagcttgg ataagaaaat acgtccttta gaagaattag aagcattatt aagtagtgga
     4501 aatacaaaac aattgaagaa caaagaggtc gctgccttgg ttattcacgg taagttacct
     4561 ttgtacgctt tggagaaaaa attaggtgat actacgagag cggttgcggt acgtaggaag
     4621 gctctttcaa ttttggcaga agctcctgta ttagcatctg atcgtttacc atataaaaat
     4681 tatgactacg accgcgtatt tggcgcttgt tgtgaaaatg ttataggtta catgcctttg
     4741 cccgttggtg ttataggccc cttggttatc gatggtacat cttatcatat accaatggca
     4801 actacagagg gttgtttggt agcttctgcc atgcgtggct gtaaggcaat caatgctggc
     4861 ggtggtgcaa caactgtttt aactaaggat ggtatgacaa gaggcccagt agtccgtttc
     4921 ccaactttga aaagatccgg tgcctgtaag atatggttag actcagaaga gggacaaaac
     4981 gcaattaaaa aagcttttaa ctctacatca agatttgcac gtctgcaaca tattcaaact
     5041 tgtctagcag gagatttact cttcatgaga tttagaacaa ctactggtga cgcaatgggt
     5101 atgaatatga tttctaaagg tgtcgaatac tcattaaagc aaatggtaga agagtatggc
     5161 tgggaagata tggaggttgt ctccgtttct ggtaactact gtaccgacaa aaaaccagct
     5221 gccatcaact ggatcgaagg tcgtggtaag agtgtcgtcg cagaagctac tattcctggt
     5281 gatgttgtca gaaaagtgtt aaaaagtgat gtttccgcat tggttgagtt gaacattgct
     5341 aagaatttgg ttggatctgc aatggctggg tctgttggtg gatttaacgc acatgcagct
     5401 aatttagtga cagctgtttt cttggcatta ggacaagatc ctgcacaaaa tgttgaaagt
     5461 tccaactgta taacattgat gaaagaagtg gacggtgatt tgagaatttc cgtatccatg
     5521 ccatccatcg aagtaggtac catcggtggt ggtactgttc tagaaccaca aggtgccatg
     5581 ttggacttat taggtgtaag aggcccgcat gctaccgctc ctggtaccaa cgcacgtcaa
     5641 ttagcaagaa tagttgcctg tgccgtcttg gcaggtgaat tatccttatg tgctgcccta
     5701 gcagccggcc atttggttca aagtcatatg acccacaaca ggaaacctgc tgaaccaaca
     5761 aaacctaaca atttggacgc cactgatata aatcgtttga aagatgggtc cgtcacctgc
     5821 attaaatcct gaggatctag gaggttaatt ggatgtcatt accgttctta acttctgcac
     5881 cgggaaaggt tattattttt ggtgaacact ctgctgtgta caacaagcct gccgtcgctg
     5941 ctagtgtgtc tgcgttgaga acctacctgc taataagcga gtcatctgca ccagatacta
     6001 ttgaattgga cttcccggac attagcttta atcataagtg gtccatcaat gatttcaatg
     6061 ccatcaccga ggatcaagta aactcccaaa aattggccaa ggctcaacaa gccaccgatg
     6121 gcttgtctca ggaactcgtt agtcttttgg acccgttgtt agctcaacta tccgaatcct
     6181 tccactacca tgcagcgttt tgtttcctgt atatgtttgt ttgcctatgc ccccatgcca
     6241 agaatattaa gttttcttta aagtctactt tacccatcgg tgctgggttg ggctcaagcg
     6301 cctctatttc tgtatcactg gccttagcta tggcctactt gggggggtta ataggatcta
     6361 atgacttgga aaagctgtca gaaaacgata agcatatagt gaatcaatgg gccttcatag
     6421 gtgaaaagtg tattcacggt accccttcag gaatagataa cgctgtggcc acttatggta
     6481 atgccctgct atttgaaaaa gactcacata atggaacaat aaacacaaac aattttaagt
     6541 tcttagatga tttcccagcc attccaatga tcctaaccta tactaggatt ccaaggtcta
     6601 caaaagacct tgttgctcgc gttcgtgtgt tggtcaccga gaaatttcct gaagttatga
     6661 agccaattct agatgccatg ggtgaatgtg ccctacaagg cttagagatc atgactaagt
     6721 taagtaaatg taaaggcacc gatgacgagg ctgtagaaac taataatgaa ctgtatgaac
     6781 aactattgga attgataaga ataaatcatg gactgcttgt ctcaatcggt gtttctcatc
     6841 ctggattaga acttattaaa aatctgagcg atgatttgag aattggctcc acaaaactta
     6901 ccggtgctgg tggcggcggt tgctctttga ctttgttacg aagagacatt actcaagagc
     6961 aaattgacag cttcaaaaag aaattgcaag atgattttag ttacgagaca tttgaaacag
     7021 acttgggtgg gactggctgc tgtttgttaa gcgcaaaaaa tttgaataaa gaccttaaaa
     7081 tcaaatccct agtattccaa ttatttgaaa ataaaactac cacaaagcaa caaattgacg
     7141 atctattatt gccaggaaac acgaatttac catggacttc atagggatct taaggaggat
     7201 accctatgtc agagttgaga gccttcagtg ccccagggaa agcgttacta gctggtggat
     7261 atttagtttt agatacaaaa tatgaagcat ttgtagtcgg attatcggca agaatgcatg
     7321 ctgtagccca tccttacggt tcattgcaag ggtctgataa gtttgaagtg cgtgtgaaaa
     7381 gtaaacaatt taaagatggg gagtggctgt accatataag tcctaaaagt ggcttcattc
     7441 ctgtttcgat aggcggatct aagaaccctt tcattgaaaa agttatcgct aacgtattta
     7501 gctactttaa acctaacatg gacgactact gcaatagaaa cttgttcgtt attgatattt
     7561 tctctgatga tgcctaccat tctcaggagg atagcgttac cgaacatcgt ggcaacagaa
     7621 gattgagttt tcattcgcac agaattgaag aagttcccaa aacagggctg ggctcctcgg
     7681 caggtttagt cacagtttta actacagctt tggcctcctt ttttgtatcg gacctggaaa
     7741 ataatgtaga caaatataga gaagttattc ataatttagc acaagttgct cattgtcaag
     7801 ctcagggtaa aattggaagc gggtttgatg tagcggcggc agcatatgga tctatcagat
     7861 atagaagatt cccacccgca ttaatctcta atttgccaga tattggaagt gctacttacg
     7921 gcagtaaact ggcgcatttg gttgatgaag aagactggaa tattacgatt aaaagtaacc
     7981 atttaccttc gggattaact ttatggatgg gcgatattaa gaatggttca gaaacagtaa
     8041 aactggtcca gaaggtaaaa aattggtatg attcgcatat gccagaaagc ttgaaaatat
     8101 atacagaact cgatcatgca aattctagat ttatggatgg actatctaaa ctagatcgct
     8161 tacacgagac tcatgacgat tacagcgatc agatatttga gtctcttgag aggaatgact
     8221 gtacctgtca aaagtatcct gaaatcacag aagttagaga tgcagttgcc acaattagac
     8281 gttcctttag aaaaataact aaagaatctg gtgccgatat cgaacctccc gtacaaacta
     8341 gcttattgga tgattgccag accttaaaag gagttcttac ttgcttaata cctggtgctg
     8401 gtggttatga cgccattgca gtgattacta agcaagatgt tgatcttagg gctcaaaccg
     8461 ctaatgacaa aagattttct aaggttcaat ggctggatgt aactcaggct gactggggtg
     8521 ttaggaaaga aaaagatccg gaaacttatc ttgataaata gggatctagg aggattatga
     8581 gatgaccgtt tacacagcat ccgttaccgc acccgtcaac atcgcaaccc ttaagtattg
     8641 ggggaaaagg gacacgaagt tgaatctgcc caccaattcg tccatatcag tgactttatc
     8701 gcaagatgac ctcagaacgt tgacctctgc ggctactgca cctgagtttg aacgcgacac
     8761 tttgtggtta aatggagaac cacacagcat cgacaatgaa agaactcaaa attgtctgcg
     8821 cgacctacgc caattaagaa aggaaatgga atcgaaggac gcctcattgc ccacattatc
     8881 tcaatggaaa ctccacattg tctccgaaaa taactttcct acagcagctg gtttagcttc
     8941 ctccgctgct ggctttgctg cattggtctc tgcaattgct aagttatacc aattaccaca
     9001 gtcaacttca gaaatatcta gaatagcaag aaaggggtct ggttcagctt gtagatcgtt
     9061 gtttggcgga tacgtggcct gggaaatggg aaaagctgaa gatggtcatg attccatggc
     9121 agtacaaatc gcagacagct ctgactggcc tcagatgaaa gcttgtgtcc tagttgtcag
     9181 cgatattaaa aaggatgtga gttccactca gggtatgcaa ttgaccgtgg caacctccga
     9241 actatttaaa gaaagaattg aacatgtcgt accaaagaga tttgaagtca tgcgtaaagc
     9301 cattgttgaa aaagatttcg ccacctttgc aaaggaaaca atgatggatt ccaactcttt
     9361 ccatgccaca tgtttggact ctttccctcc aatattctac atgaatgaca cttccaagcg
     9421 tatcatcagt tggtgccaca ccattaatca gttttacgga gaaacaatcg ttgcatacac
     9481 gtttgatgca ggtccaaatg ctgtgttgta ctacttagct gaaaatgagt cgaaactctt
     9541 tgcatttatc tataaattgt ttggctctgt tcctggatgg gacaagaaat ttactactga
     9601 gcagcttgag gctttcaacc atcaatttga atcatctaac tttactgcac gtgaattgga
     9661 tcttgagttg caaaaggatg ttgccagagt gattttaact caagtcggtt caggcccaca
     9721 agaaacaaac gaatctttga ttgacgcaaa gactggtcta ccaaaggaat aaggatctag
     9781 gaggtaatga taatgcaaac ggaacacgtc attttattga atgcacaggg agttcccacg
     9841 ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct cgcgttctcc
     9901 agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact gagcaaaaaa
     9961 gcatggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg agaaagcaac
    10021 gaagacgcag tgatccgccg ttgccgttat gagcttggcg tggaaattac gcctcctgaa
    10081 tctatctatc ctgactttcg ctaccgcgcc accgatccga gtggcattgt ggaaaatgaa
    10141 gtgtgtccgg tatttgccgc acgcaccact agtgcgttac agatcaatga tgatgaagtg
    10201 atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc cacgccgtgg
    10261 gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa acgattatct
    10321 gcatttaccc agcttaaata aggatctagg aggttactca tatggacttt ccgcagcaac
    10381 tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct
    10441 ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc
    10501 tgcgaccttt cctggtttat gccaccggtc atatgttcgg cgttagcaca aacacgctgg
    10561 acgcacccgc tgccgccgtt gagtgtatcc acgcttactc attaattcat gatgatttac
    10621 cggcaatgga tgatgacgat ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg
    10681 aagcaaacgc gattctcgct ggcgacgctt tacaaacgct ggcgttctcg attttaagcg
    10741 atgccgatat gccggaagtg tcggaccgcg acagaatttc gatgatttct gaactggcga
    10801 gcgccagtgg tattgccgga atgtgcggtg gtcaggcatt agatttagac gcggaaggca
    10861 aacacgtacc tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc
    10921 gcgccgccgt tcgccttggt gcattaagcg ccggagataa aggacgtcgt gctctgccgg
    10981 tactcgacaa gtatgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg
    11041 atgtggtggg agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta
    11101 aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga
    11161 tcgacgatgc ccgtcagtcg ctgaaacaac tggctgaaca gtcactcgat acctcggcac
    11221 tggaagcgct agcggactac atcatccagc gtaataaata aggatctcca ggcatcaaat
    11281 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
    11341 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggat
    11401 cctaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
    11461 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
    11521 accttcgggt gggcctttct gcgtttatac ctagggatat attccgcttc ctcgctcact
    11581 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
    11641 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
    11701 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
    11761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
    11821 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
    11881 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
    11941 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12001 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
    12061 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
    12121 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
    12181 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
    12241 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt
    12301 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt tactagtgct
    12361 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
    12421 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
    12481 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
    12541 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
    12601 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
    12661 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
    12721 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
    12781 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
    12841 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
    12901 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
    12961 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
    13021 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
    13081 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
    13141 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
    13201 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga
    13261 tatc
//
