LOCUS       sequence-432644-        7383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB719
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1751..1938
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1806..1830)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2414..2821
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2822..3916
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4947..5762)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4947..5762)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5670..5689
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5754..5773
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5987..6575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6476..6495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga atgaaaacga
      721 gtacgataat tttgatgatt tggatgacct tttagatgaa gatcccacta aactggatga
      781 agcagagccc gatgatgtgc aagcgaaggg ttctgtgtac aacgatagtg aaaacaaaga
      841 gaagaatgcc gaaagtaagg acagtgatgg cgtacaggta gctaatgaat ctgaagagga
      901 cccagaattg aaagaaatga tggtagattt gcaaaatgag ttcgcaaatt tgatgaaaaa
      961 taacggtaac gagaataatg tcaaaacaga agattttaat aaactgatta gtgctctaga
     1021 agaggcagct aaagtacctc atcaacagat ggagcaagga tgtagtagtt tgaaaagtaa
     1081 cagcactgat aaagggaccg tgaatggtag caatccggga tttaaaaaca ttgtttccaa
     1141 tacgctagac aggttaaagg aaaatggtaa taaagttgat acatccctag ccgaagaaac
     1201 caaggaatct caacgcagtg gccaaaataa taatattgat gatatattgt cacaattatt
     1261 agatcaaatg gtagcatctg gtggaaaaga aagtgcagaa aatcaatttg atctaaaaga
     1321 tggggagatg gacgacgcaa taacaaaaat acttgatcaa atgacgtcaa aagaagtatt
     1381 atatgagcct atgaaagaaa tgcgtagtga gttcggtgtt tggtttcaag aaaacggtga
     1441 aaatgaggaa cataaggaaa agataggcac ctacaagagg caatttaata tcgtagacga
     1501 aattgtaaat atttatgaat tgaaagatta tgacgagcta aagcataaag atcgtgttac
     1561 tgaacttttg gacgaattag aacagcttgg tgatagccct attagaagtg cgaacagccc
     1621 cctaaaacac ggtaatgaag aagaagaact catgaaaatg ttggaaattg atggtaatga
     1681 tccgaacttg gggaaccttg ataaggaatt aaccgacggt tgcaaacaac aataaggatc
     1741 ctaactcgag gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     1801 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     1861 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     1921 tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctgg
     1981 aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata ccgatgactg
     2041 cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca ccactgcata
     2101 tatggcatat cacaacagtc cacgtctcaa gcagttacag agatgttacg aaccactagt
     2161 gcactgcagt acatcgagga gaacttctag tatatctaca tacctaatat tattgcctta
     2221 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
     2281 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
     2341 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
     2401 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     2461 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     2521 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     2581 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
     2641 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     2701 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     2761 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
     2821 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
     2881 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
     2941 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
     3001 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
     3061 taaatgggga accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
     3121 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
     3181 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
     3241 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
     3301 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
     3361 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
     3421 attatggaga aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaggttca
     3481 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     3541 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     3601 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     3661 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
     3721 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     3781 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     3841 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     3901 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     3961 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     4021 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     4081 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     4141 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     4201 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     4261 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
     4321 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     4381 acgacgtagt cgagagtact cgtatcgcat gtcggtgcga cacgaaatta caaaatggaa
     4441 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     4501 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     4561 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     4621 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     4681 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     4741 taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac
     4801 tctgtcagaa acggccttaa cgacgtagtc gacctcctct tcagtactaa atctaccaat
     4861 accaaatctg atggaagaat gggctaatgc atcatcctta cccagcgccg agcggccgcg
     4921 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     4981 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     5041 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     5101 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     5161 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     5221 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     5281 accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta
     5341 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     5401 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     5461 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     5521 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     5581 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     5641 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     5701 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
     5761 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     5821 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     5881 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
     5941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6661 ctgtggataa ccgtgcggcc gccaataccc agaagaacag taaaataaag caaggtacgt
     6721 gaaattaata tttttaaatg gttctaaccg atgccgaaga actgcgcagt ccggttataa
     6781 cgtctgacat gtcctttttt gatttggaat ccaaccactc aagtgactct gttcatttac
     6841 tttgcgaaaa atatacccac aaattgccca tcgaaagtga atcgcaaacc accttcagac
     6901 tggcaccgac aaagcaaaga ttatacagac agagtacttt atacgtaccg ttaagtctca
     6961 agcaaagggt tttcttattt actgaacggg taaagagtat ctgggccggc ttgccaagat
     7021 gcaaaccgaa taagtatttc aaagttgcat ttgccttagc cgtcctgaca ccattggcta
     7081 tttggatatt ttatattgac tttcgtgtac attgatcaca tcgactgttc tattggcaaa
     7141 tgaaccacgg gcattgacta tttttcaggt tactactata tattatcatc acgggcaagg
     7201 attgtaccct gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta
     7261 caagcaacga tctccaggac catctgaatc atgcgcggat gacacgaact cacgacggcg
     7321 atcacagaca ttaacccaca gtacagacac tgcgacaacg tggcaattcg tcgcaataca
     7381 acg
//
