LOCUS       sequence-432646-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, 
            Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher 
            T, Hasan SS
  TITLE     A single C-terminal residue controls SARS-CoV-2 spike trafficking 
            and incorporation into VLPs.
  JOURNAL   Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3.
  PUBMED    38102143
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-15"; volume: "14(1):8358. doi"; pages: " 
            10.1038/s41467-023-44076-3"
COMMENT     Sequence Label: SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             846..875
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             891..908
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(931..948)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    937..1161
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1207..1635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1294..1313)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1504..1525
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1644..1664)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1649..1978
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1829..1964
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1891..1910
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2045..2839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2099..2118)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2709..2728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3015..3136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3052..3071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3106..3125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3185..3201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3185..3201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3198..3220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3209..3225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3233..3239,3240..3257,3258..3263))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3278..3299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3416..3433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3587..4175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3667..3686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4346..5137,5138..5206))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4969..4988
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5207..5311)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5386..5405)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5577..5956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5957..6160
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6111..6131
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6205..6224
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6205..6223
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggctccag agcctccaga cctatgtgac acagcagctg atcagggccg ccgagatcag
       61 ggccagcgcc aatctggcag caaccaagat gtccgagtgc gtgctgggcc agtctaagag
      121 agtggacttt tgtggcaagg gctatcacct gatgtccttc cctcagtctg ccccacacgg
      181 cgtggtgttt ctgcacgtga cctacgtgcc cgcccaggag aagaacttca ccacagcccc
      241 tgccatctgc cacgatggca aggcccactt tccaagggag ggcgtgttcg tgtccaacgg
      301 cacccactgg tttgtgacac agcgcaattt ctacgagccc cagatcatca ccacagacaa
      361 caccttcgtg agcggcaact gtgacgtggt catcggcatc gtgaacaata ccgtgtatga
      421 tccactccag cccgagctgg acagctttaa ggaggagctg gataagtatt tcaagaatca
      481 cacctcccct gacgtggatc tgggcgacat cagcggcatc aatgcctccg tggtgaacat
      541 ccagaaggag atcgaccgcc tgaacgaggt ggctaagaat ctgaacgaga gcctgatcga
      601 cctccaggag ctgggcaagt atgagcagta catcaagtgg ccctggtaca tctggctggg
      661 cttcatcgcc ggcctgatcg ccatcgtgat ggtgaccatc atgctgtgct gtatgacatc
      721 ctgctgttct tgcctgaagg gctgctgtag ctgtggctcc tgctgtaagt ttgacgagga
      781 tgactctgaa cctgtgctga agggcgtgaa gctgcattac acctaaatcg gatcccgggc
      841 ccttcgaaca aaaactcatc tcagaagagg atctgaatat gcataccggt catcatcacc
      901 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      961 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     1021 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     1081 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     1141 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     1201 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1261 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1381 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1441 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1501 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1561 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1621 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1801 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1861 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     1921 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1981 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     2041 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     2101 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     2161 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     2221 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     2281 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     2341 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     2401 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     2461 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     2521 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     2581 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     2641 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     2701 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     2761 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     2821 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
     2881 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     2941 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     3001 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3121 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     3181 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3241 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3301 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3361 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3421 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3481 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3541 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4021 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5341 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     5401 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     5461 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     5521 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     5581 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5641 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     5701 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     5761 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     5821 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     5881 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     5941 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     6001 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     6061 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     6121 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     6181 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     6241 gttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc gagctcggta
     6301 cctcgcgaat gcatctagat gccaccatgt tcgtcttcct ggtcctgctg cctctggtct
     6361 cctcacagtg cgtcaatctg acaactcgga ctcagctgcc acctgcttat actaatagct
     6421 tcaccagagg cgtgtactat cctgacaagg tgtttagaag ctccgtgctg cactctacac
     6481 aggatctgtt tctgccattc tttagcaacg tgacctggtt ccacgccatc cacgtgagcg
     6541 gcaccaatgg cacaaagcgg ttcgacaatc ccgtgctgcc ttttaacgat ggcgtgtact
     6601 tcgcctctac cgagaagagc aacatcatca gaggctggat ctttggcacc acactggact
     6661 ccaagacaca gtctctgctg atcgtgaaca atgccaccaa cgtggtcatc aaggtgtgcg
     6721 agttccagtt ttgtaatgat cccttcctgg gcgtgtacta tcacaagaac aataagagct
     6781 ggatggagtc cgagtttaga gtgtattcta gcgccaacaa ctgcacattt gagtacgtga
     6841 gccagccttt cctgatggac ctggagggca agcagggcaa tttcaagaac ctgagggagt
     6901 tcgtgtttaa gaatatcgac ggctacttca aaatctactc taagcacacc cccatcaacc
     6961 tggtgcgcga cctgcctcag ggcttcagcg ccctggagcc cctggtggat ctgcctatcg
     7021 gcatcaacat cacccggttt cagacactgc tggccctgca cagaagctac ctgacacccg
     7081 gcgactcctc tagcggatgg accgccggcg ctgccgccta ctatgtgggc tacctccagc
     7141 cccggacctt cctgctgaag tacaacgaga atggcaccat cacagacgca gtggattgcg
     7201 ccctggaccc cctgagcgag acaaagtgta cactgaagtc ctttaccgtg gagaagggca
     7261 tctatcagac atccaatttc agggtgcagc caaccgagtc tatcgtgcgc tttcctaata
     7321 tcacaaacct gtgcccattt ggcgaggtgt tcaacgcaac ccgcttcgcc agcgtgtacg
     7381 cctggaatag gaagcggatc agcaactgcg tggccgacta tagcgtgctg tacaactccg
     7441 cctctttcag cacctttaag tgctatggcg tgtcccccac aaagctgaat gacctgtgct
     7501 ttaccaacgt ctacgccgat tctttcgtga tcaggggcga cgaggtgcgc cagatcgccc
     7561 ccggccagac aggcaagatc gcagactaca attataagct gccagacgat ttcaccggct
     7621 gcgtgatcgc ctggaacagc aacaatctgg attccaaagt gggcggcaac tacaattatc
     7681 tgtaccggct gtttagaaag agcaatctga agcccttcga gagggacatc tctacagaaa
     7741 tctaccaggc cggcagcacc ccttgcaatg gcgtggaggg ctttaactgt tatttcccac
     7801 tccagtccta cggcttccag cccacaaacg gcgtgggcta tcagccttac cgcgtggtgg
     7861 tgctgagctt tgagctgctg cacgccccag caacagtgtg cggccccaag aagtccacca
     7921 atctggtgaa gaacaagtgc gtgaacttca acttcaacgg cctgaccggc acaggcgtgc
     7981 tgaccgagtc caacaagaag ttcctgccat ttcagcagtt cggcagggac atcgcagata
     8041 ccacagacgc cgtgcgcgac ccacagaccc tggagatcct ggacatcaca ccctgctctt
     8101 tcggcggcgt gagcgtgatc acacccggca ccaatacaag caaccaggtg gccgtgctgt
     8161 atcaggacgt gaattgtacc gaggtgcccg tggctatcca cgccgatcag ctgaccccaa
     8221 catggcgggt gtacagcacc ggctccaacg tcttccagac aagagccgga tgcctgatcg
     8281 gagcagagca cgtgaacaat tcctatgagt gcgacatccc aatcggcgcc ggcatctgtg
     8341 cctcttacca gacccagaca aactctccca gaagagcccg gagcgtggcc tcccagtcta
     8401 tcatcgccta taccatgtcc ctgggcgccg agaacagcgt ggcctactct aacaatagca
     8461 tcgccatccc aaccaacttc acaatctctg tgaccacaga gatcctgccc gtgtccatga
     8521 ccaagacatc tgtggactgc acaatgtata tctgtggcga ttctaccgag tgcagcaacc
     8581 tgctgctcca gtacggcagc ttttgtaccc agctgaatag agccctgaca ggcatcgccg
     8641 tggagcagga taagaacaca caggaggtgt tcgcccaggt gaagcaaatc tacaagaccc
     8701 cccctatcaa ggactttggc ggcttcaatt tttcccagat cctgcctgat ccatccaagc
     8761 cttctaagcg gagctttatc gaggacctgc tgttcaacaa ggtgaccctg gccgatgccg
     8821 gcttcatcaa gcagtatggc gattgcctgg gcgacatcgc agccagggac ctgatctgcg
     8881 cccagaagtt taatggcctg accgtgctgc cacccctgct gacagatgag atgatcgcac
     8941 agtacacaag cgccctgctg gccggcacca tcacatccgg atggaccttc ggcgcaggag
     9001 ccgccctcca gatccccttt gccatgcaga tggcctatag gttcaacggc atcggcgtga
     9061 cccagaatgt gctgtacgag aaccagaagc tgatcgccaa tcagtttaac tccgccatcg
     9121 gcaagatcca ggacagcctg tcctctacag ccagcgccct gggcaagctc caggatgtgg
     9181 tgaatcagaa cgcccaggcc ctgaataccc tggtgaagca gctgagcagc aacttcggcg
     9241 ccatctctag cgtgctgaat gacatcctga gccggctgga caaggtggag gcagaggtgc
     9301 agatcgaccg gctgatcacc gg
//
