LOCUS       sequence-432669-        5793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV reporter genome plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes 
            C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G,
            Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen
            AT, Barry AJ, Eid FE, Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human transferrin receptor 
            mediates brain-wide gene delivery.
  JOURNAL   Science. 2024 Jun 14;384(6701):1220-1227. doi: 
            10.1126/science.adm8386. Epub 2024 May 16.
  PUBMED    38753766
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adm8386"; journalName: "Science"; date: 
            "2024-06-14- 14"; volume: "384"; issue: "6701"; pages: "1220-1227"
COMMENT     Sequence Label: pAAV-CMV-CBA-hGBA1-HA-hGHpA
FEATURES             Location/Qualifiers
     source          1..5793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(269..295)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1900..1909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2258..2561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   2641..2770
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(2832..3420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2912..2931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3591..4382,4383..4451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4214..4233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4452..4556)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4624..4642
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4680..4702)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4802..4821
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4838..5293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5187..5206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5368..5508
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5379..5508
                     /label=AAV2 ITR
     polyA_signal    join(5548..5793,1..231)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
ORIGIN
        1 tgccactgca ctccagcttg gttcccaata gaccccgcag gccctacagg ttgtcttccc
       61 aacttgcccc ttgctccata ccacccccct ccaccccata atattataga aggacaccta
      121 gtcagacaaa atgatgcaac ttaattttat taggacaagg ctggtgggca ctggagtggc
      181 aacttccagg gccaggagag gcactgggga ggggtcacag ggatgccacc cgtagatctc
      241 tcgagcagcg ctttgatatc gaattctatg catagtctgg gacgtcatat ggatactggc
      301 gacgccacag gtaggtgtga atggagtagc caggtgagat tgtctccagg aagcccacag
      361 caggatcctt gatggtaaga ggcacatcct tagaggagcg gtttagcacg accacaacag
      421 cagagccatc gggatgcatc agtgccactg cgtccaggtc gttcttctga ctggcaacca
      481 gccccactct ctgggagccc tcaggaatga acttgctgaa gtggccaagg tggtagaaca
      541 tgggctgttt gtaaaacgtg tccttggtga tgtctacaat gatgggactg tcgacaaagt
      601 tacgcaccca attgggtcct ccttcggggt tcagggcaag gttccagtcg gtccagccga
      661 ccacatggta caggaggttc gtgatgatgc tgtggctgta ctgcatccct cgatcccagg
      721 agcctagccg cacactctgc tcccagaact tggagcccac acaggcctct gaggcaaaga
      781 gcatggtgtt ggggaacagg cggtgtgtct cccctagggt ggctttggct ggagccagaa
      841 agtccaggta ccaatgtaca gcaatgccat gaacatattt agctgcttct gggtctgtca
      901 gtaccacctt tgcccagtgg ggcagcagca agcgttggtc atccagcatg agtaggcgga
      961 cattgtggtg agtactgttg gcgagggtag gacctaggtc acgggcaatg aagtctcgct
     1021 gatgttcagg ggtgaagccc aggcactgga aggggtatcc actcaacagc ccagcagaag
     1081 gctcattttc agctgtcact gcccagaact gtaacttgtg ctcagcatag gcatccagga
     1141 acttcacaaa gtatctggcc caggtctggt ggtagatgtc tccgggctgt cccttgagtg
     1201 accccttccc attcaccgct ccattggtct tgagccaagt gggtgatgtc caggggctgg
     1261 caaggagtga aacgggacgc tgggccaact gcagggctcg gtgaatcagg ggtatcttga
     1321 gcttggtatc ttcctctggg aggctgaagt tgtgcaactg gaaatcatca ggggtgtctg
     1381 cataggtgta ggtgcggatg gagaagtcac agctggccat gggtacccgg atgatgttat
     1441 atccgattcc ttcttcagag aagtacgatt taagtagcaa attttgggca gggggtgaca
     1501 gggcaaggat gttgagagca gcagcatctg tcatggcccc tccaaatccc ttcactttct
     1561 ggaacttctg ttctggctgc agggtcagta gcaggcctgt gcccgtgtga ttagcctgga
     1621 tgggccccat actcagctcc atccgtcgcc cactgcgtgt actctcatag cggctgaagg
     1681 taccaagggc aggaaaggtc ggggggtcaa aggagtcaca gtatgtggca ttgcagacac
     1741 acaccaccga gctgtagccg aagcttttag ggatgcaggg gcgggcacct gatgcccacg
     1801 acactgcctg aagtagaagc aatcctgtga ggctgccagc catgatgctt accctactca
     1861 aaggcttggg acattcctct ctggaaggac ttgaaaactc catggtggcg gtaccgttgg
     1921 gccgcgcccg ccgcgcgctt cgctttttat agggccgccg ccgcccccgc ctcgccataa
     1981 aaggaaactt tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc
     2041 cgccccgccc ctcgccccgc cccgccccgc ctcgcgcgcg cccccccccc cccccccccc
     2101 cccccccccc cccccccccc cccccccggg gcgcgccccc cccccccccc gcccccatcg
     2161 ctgcacaaaa taattaaaaa ataaataaat acaaaattgg gggtggggag gggggggaga
     2221 tggggagagt gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata
     2281 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     2341 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     2401 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     2461 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     2521 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     2581 tatgaactaa tgaccccgta attgattact attaataact agtgttacgc gtgcggccgc
     2641 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     2701 ccgggcgacc aaaggtcgcc cgacgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     2761 tgcctgcagg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4561 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4621 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4681 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4741 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4801 gcggcatcag agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg
     4861 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata gaccgaaatc
     4921 ggcaaaatcc cttataaatc aaaagaatag cccgagatag agttgagtgt tgttccagtt
     4981 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5041 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
     5101 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5161 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg
     5221 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5281 ctacagggcg cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg
     5341 taaggagaaa ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg
     5401 aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg
     5461 agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc ggccgctcgg
     5521 tccgcacgtg gttacctaca aaatcagaag gacagggaag ggagcagtgg ttcacgcctg
     5581 taatcccagc aatttgggag gccaaggtgg gtagatcacc tgagattagg agttggagac
     5641 cagcctggcc aatatggtga aaccccgtct ctaccaaaaa aacaaaaatt agctgagcct
     5701 ggtcatgcat gcctggaatc ccaacaactc gggaggctga ggcaggagaa tcgcttgaac
     5761 ccaggaggcg gagattgcag tgagccaaga ttg
//
