LOCUS       sequence-432670-        5712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV reporter genome plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes 
            C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G,
            Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen
            AT, Barry AJ, Eid FE, Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human transferrin receptor 
            mediates brain-wide gene delivery.
  JOURNAL   Science. 2024 Jun 14;384(6701):1220-1227. doi: 
            10.1126/science.adm8386. Epub 2024 May 16.
  PUBMED    38753766
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adm8386"; journalName: "Science"; date: 
            "2024-06-14- 14"; volume: "384"; issue: "6701"; pages: "1220-1227"
COMMENT     Sequence Label: pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA
FEATURES             Location/Qualifiers
     source          1..5712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   119..259
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   119..248
                     /label=AAV2 ITR
     rep_origin      334..789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(421..440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     631..652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(806..825)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     925..947
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(985..1003)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1071..1175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1176..1244,1245..2036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1394..1413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2207..2795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2696..2715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2857..2986
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        3066..3369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3371..3647
                     /label=chicken beta-actin promoter
     regulatory      3663..3672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    join(5315..5712,1..79)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5514..5533)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
       61 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
      121 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
      181 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      241 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      301 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
      361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
      421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      541 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      661 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
      721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
      781 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      841 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      901 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      961 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1021 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1081 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1141 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1201 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1261 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1321 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1381 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1441 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1501 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1561 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1621 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1681 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1741 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1801 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1861 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1921 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1981 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2041 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2821 ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct
     2881 cgctcactga ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
     2941 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgcacgcgt
     3001 aacactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     3061 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     3121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     3181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     3241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     3301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     3361 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     3421 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     3481 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     3541 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     3601 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgcgg cccaacggta
     3661 ccgccaccat ggagttcagc tccccctcca gggaggagtg ccctaaacca ctgtcaagag
     3721 tctctatcat ggccggatcc ctcaccggcc tgctgctgct gcaggccgtg agctgggcct
     3781 ctggcgctag accatgcatc cctaagtcct tcggctactc ctccgtggtg tgcgtgtgca
     3841 acgccaccta ctgcgactcc ttcgaccctc ctactttccc cgctctgggg accttcagcc
     3901 ggtacgagtc cacccgaagt ggcaggcgca tggagctgag catggggcct atccaggcta
     3961 accacacagg aacaggcctg ctgctgaccc tgcagcccga acagaagttc cagaaggtga
     4021 agggctttgg aggcgccatg accgacgctg ctgccctgaa cattctcgcc ctgtcccccc
     4081 ccgctcagaa cctgctcctg aagagctact tcagcgaaga agggatcggg tacaacatca
     4141 tcagggtgcc catggccagc tgcgactttt ccattagaac atacacctat gctgatactc
     4201 ccgatgactt tcagctgcac aactttagcc tgccagaaga ggacacaaag ctgaagatcc
     4261 ccctgatcca cagggccctg cagctcgctc agagacctgt gtccctgctg gccagcccat
     4321 ggacaagtcc cacctggctg aaaaccaacg gcgccgtgaa cggcaagggt tccctgaagg
     4381 ggcagcccgg agacatctac catcagacct gggcaaggta ctttgtgaaa ttcctggacg
     4441 cctatgccga gcacaaactg cagttctggg ctgtgactgc tgagaacgag ccttccgcag
     4501 gactgctgag cggataccct ttccagtgtc tgggctttac ccctgagcac cagcgcgact
     4561 tcatcgctcg ggacctggga cccaccctgg ctaattccac ccaccacaac gtgagactgc
     4621 tgatgctgga cgatcagaga ctgctgctgc cacactgggc caaggtggtg ctgactgacc
     4681 cagaggccgc aaagtacgtg cacggcatcg ctgtgcactg gtacctggat tttctggctc
     4741 cagccaaggc taccctgggc gaaacacacc ggctgttccc taacacaatg ctgttcgcct
     4801 ccgaggcctg cgtgggctcc aagttctggg agcagagcgt gagactgggt agctgggaca
     4861 gaggcatgca gtacagccac tcaatcatta caaatctcct gtaccacgtg gtggggtgga
     4921 ccgattggaa tctggctctg aaccccgagg gaggaccaaa ctgggtgcgc aacttcgtgg
     4981 atagcccaat catcgtggac atcaccaaag ataccttcta taagcagcct atgttttatc
     5041 acctgggcca tttttccaag ttcatcccag agggctcaca gcgggtaggc ctggttgcca
     5101 gccagaagaa cgacctggac gccgtggcac tcatgcaccc cgatgggtct gccgtggtgg
     5161 tggtgctgaa cagatcctct aaggacgtgc ccctgactat taaggaccca gccgtggggt
     5221 tcctggagac aatctctcca ggctacagca tccacaccta cctgtggcgg agacagtaag
     5281 aattcgatat caaagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc
     5341 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     5401 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     5461 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     5521 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     5581 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     5641 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     5701 ctccaactcc ta
//
