LOCUS       sequence-432723-        3442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector containing the gpdA promoter for Golden Gate cloning in the 
            Fg vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Darino M, Urban M, Kaur N, Machado Wood A, Grimwade-Mann M, Smith D,
            Beacham A, Hammond-Kosack K
  TITLE     Identification and functional characterisation of a locus for target
            site integration in Fusarium graminearum.
  JOURNAL   Fungal Biol Biotechnol. 2024 Feb 26;11(1):2. doi: 
            10.1186/s40694-024-00171-8.
  PUBMED    38409036
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2024-02-26"; volume: "11(1):2. doi"; pages: " 
            10.1186/s40694-024-00171-8"
COMMENT     Sequence Label: pJET-PgpdA
FEATURES             Location/Qualifiers
     source          1..3442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1253..1269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1277..1283,1284..1301,1302..1307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1322..1343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1460..1477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1631..2219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1711..1730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2390..3181,3182..3250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3013..3032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3251..3355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3423..3441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tggtctcatg tttttgaaga ttgggttcct ctttgagaga gttaatagac
      421 ttgaattgag gaatggaaat cgggctgagc tcactgcttt atatgtgaga aaaggtgatg
      481 agcaggtggt aagaggcaga tttttgtggt cggtgctgtg ttttgttggg tttgagctag
      541 gtggggtgag ggaggggcaa gctaaatagc taccagccct gaggacgtca cagagtttta
      601 gtagttcttt ttctgccatc ggacatgcag ctcacgccta gacccaatta caccctttgc
      661 gccctcccac acatccggcc ggttggatca ttgtcagata cggcagagaa atcgcaacct
      721 cggccaaggg tagccacgat tcgaagccgc gaaattgggg gtttcggaat gtcgtcaagc
      781 gggaaccaat ttgagtaccc aattcgccct atccaacatg gtggagtgag gaggttgaga
      841 ccatctttct agaagatctc ctacaatatt ctcagctgcc atggaaaatc gatgttcttc
      901 ttttattctc tcaagatttt caggctgtat attaaaactt atattaagaa ctatgctaac
      961 cacctcatca ggaaccgttg taggtggcgt gggttttctt ggcaatcgac tctcatgaaa
     1021 actacgagct aaatattcaa tatgttcctc ttgaccaact ttattctgca ttttttttga
     1081 acgaggttta gagcaagctt caggaaactg agacaggaat tttattaaaa atttaaattt
     1141 tgaagaaagt tcagggttaa tagcatccat tttttgcttt gcaagttcct cagcattctt
     1201 aacaaaagac gtctcttttg acatgtttaa agtttagggg agaattgtga aattgttatc
     1261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3361 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3421 aaaataggcg tatcacgagg cc
//
