LOCUS       sequence-432725-        3515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector containing the TrpC terminator for Golden Gate cloning in the
            Fg vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Darino M, Urban M, Kaur N, Machado Wood A, Grimwade-Mann M, Smith D,
            Beacham A, Hammond-Kosack K
  TITLE     Identification and functional characterisation of a locus for target
            site integration in Fusarium graminearum.
  JOURNAL   Fungal Biol Biotechnol. 2024 Feb 26;11(1):2. doi: 
            10.1186/s40694-024-00171-8.
  PUBMED    38409036
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2024-02-26"; volume: "11(1):2. doi"; pages: " 
            10.1186/s40694-024-00171-8"
COMMENT     Sequence Label: pJET-TtrpC
FEATURES             Location/Qualifiers
     source          1..3515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1315..1337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1350..1356,1357..1374,1375..1380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1395..1416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1533..1550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1784..1803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2463..3254,3255..3323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3086..3105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3324..3428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3496..3514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tggtctcata gtggtaccag ttcaaggaag aaacagtgca attatctttg
      421 cgaacccagg ggctggtgac ggaattttca tagtcaagct atcagagtaa agaagaggag
      481 catgtcaaag tacaattaga gacaaatata tagtcgcgtg gagccaagag cggattcctc
      541 agtctcgtag gtcgcttgac gaccgttgat ctgcttgatc tcgtctcccg aaaatgaaaa
      601 tagactctgc taagctattc ttctgcttcg ccggagcctg aagggcgtac tagggttgcg
      661 aggtccaatg cattaatgca ttgcagatga gctgtatctg gaagaggtaa acccgaaacg
      721 cgttttattc ttgttgacat ggagctatta aatcactaga aggcactctt tgctgcttgg
      781 acaaatgaac gtatcttatc gagatcctga acaccatttg tctcaactcc ggagctgaca
      841 tcgacaccaa cgatcttata tccagattcg tcaagctgtt tgatgatttc agtaacgtta
      901 agtggcagtg agaccatctt tctagaagat ctcctacaat attctcagct gccatggaaa
      961 atcgatgttc ttcttttatt ctctcaagat tttcaggctg tatattaaaa cttatattaa
     1021 gaactatgct aaccacctca tcaggaaccg ttgtaggtgg cgtgggtttt cttggcaatc
     1081 gactctcatg aaaactacga gctaaatatt caatatgttc ctcttgacca actttattct
     1141 gcattttttt tgaacgaggt ttagagcaag cttcaggaaa ctgagacagg aattttatta
     1201 aaaatttaaa ttttgaagaa agttcagggt taatagcatc cattttttgc tttgcaagtt
     1261 cctcagcatt cttaacaaaa gacgtctctt ttgacatgtt taaagtttat gtttcctgtg
     1321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3481 acattaacct ataaaaatag gcgtatcacg aggcc
//
