LOCUS       sequence-432728-        1461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding 
            sequence was optimised for expression and carries a C-terminal 
            3xHA-tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Zhang J, Sommermann T, Li X, Gieselmann L, de la Rosa K, Stecklum M,
            Klein F, Kocks C, Rajewsky K
  TITLE     LMP1 and EBNA2 constitute a minimal set of EBV genes for 
            transformation of human B cells.
  JOURNAL   Front Immunol. 2023 Dec 19;14:1331730. doi: 
            10.3389/fimmu.2023.1331730. eCollection 2023.
  PUBMED    38169736
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2023-12-19"; volume: "14:1331730. doi"; pages: " 
            10.3389/fimmu.2023.1331730. eCollection 2023"
COMMENT     Sequence Label: MSCV-opEBNA2-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcccacct tctatctggc tctgcacggc ggacagacct accacctgat cgtggataca
       61 gacagcctgg gcaaccctag cctgagcgtg atccctagca acccctacca agagcagctg
      121 agcgacacac ctctgatccc tctgacaatc ttcgtgggcg agaacacagg cgttccacct
      181 ccattgcctc caccgccacc gccgcctcca ccacctccgc ctccacctcc gccaccaccg
      241 ccaccgcctc cgccgccacc accttctcca ccgccgccac ctccaccacc accaccgcct
      301 caaagaagag atgcctggac acaagagccc agtcctctgg atagagatcc cctgggatac
      361 gatgtcggac acggacctct ggcctctgcc atgagaatgc tgtggatggc caactacatc
      421 gtgcggcagt ctagaggcga cagaggcctg attctgcctc agggacctca gacagctcca
      481 caggctagac tggtgcagcc tcatgtgcct ccactcagac caaccgctcc taccatcctg
      541 tctccactga gccagcctag actgacacct cctcagcctc tgatgatgcc tcctaggcct
      601 acacctccta caccactgcc tccagctaca ctgacagtgc ctccaagacc aaccaggcct
      661 accacacttc ctccaacacc tctgctgact gtgctgcaga ggcctacaga actgcagccc
      721 acaccatctc cacctagaat gcatctgcca gtgctgcacg tgcccgacca gtctatgcat
      781 cctctgacac accagagcac ccctaacgac cctgacagcc ctgagcctag aagccccacc
      841 gtgttctaca acatccctcc tatgccactg ccacctagtc agttgccacc tcctgctgct
      901 ccagctcaac ctcctcctgg cgtgatcaat gaccagcagc tgcaccatct gccatctgga
      961 cctccatggt ggcctccaat ctgtgaccct ccacagcctt ctaagaccca gggacagagc
     1021 agaggacagt cccgtggtag aggccgtgga cgtggaagag gtagaggcaa gggcaagagc
     1081 agagacaagc agagaaaacc tggcggccct tggaggcctg agccaaacac atctagccct
     1141 agcatgcctg agctgagccc tgtgctggga ctgcatcaag gacaaggtgc cggcgattct
     1201 cctactcctg gaccttctaa cgccgctcct gtgtgcagaa actctcacac cgccacacct
     1261 aacgtgtccc caatccacga gcctgagagc cacaatagcc ccgaggctcc tatcctgttt
     1321 cctgacgact ggtatcctcc tagcatcgac cctgccgacc tggacgagag ctgggattac
     1381 atcttcgaga caaccgagtc tcccagcagc gacgaggatt acgtggaagg acctagcaag
     1441 aggcccagac ctagcatcca a
//
