LOCUS       sequence-43277-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     Plasmid for gene disruption
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTN186
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..432
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     375..395
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gcatacatta tacgaagtta tattcctgca gcccccctac tccaaaaatg tcaaagatac
       61 agtctcagaa gaccaaaggg ctattgacac ttttcaacaa agggtatata atcgggaaga
      121 ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa
      181 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatgcctct
      241 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
      301 gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
      361 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
      421 ttggagagga cagcccaagc aaggaggtga tatgaagaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggagagttc agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttcttcag ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg aatcggtca
//
