LOCUS       sequence-43278-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Dmitriev PV, Vassetzky YS
  TITLE     A set of vectors for introduction of antibiotic resistance genes by 
            in vitro Cre-mediated recombination.
  JOURNAL   BMC Res Notes. 2008 Dec 23;1:135. doi: 10.1186/1756-0500-1-135.
  PUBMED    19105832
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2008-12-23"; volume: "1:135. doi"; pages: " 
            10.1186/1756-0500-1-135"
COMMENT     Sequence Label: pINS-Puro
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    243..276
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      329..685
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacaa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
      181 gacaccagga tttatttatt ctgcgaagtg atcttccgtc actcgagccc ggggaattcc
      241 agataacttc gtataatgta tgctatacga agttatggta ccgcggccgc gtagaggatc
      301 tgttgatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta
      361 agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac
      421 gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg
      481 tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt
      541 ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca
      601 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg
      661 acacaggaac acttaacggc tgacatggga attagcttca cgctgccgca agcactcagg
      721 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
      781 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
      841 aaagcaggta gcttgcagtg gg
//
