LOCUS       sequence-432785-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G 
            ,P681H for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 B.1.621 (Mu)
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3842..4430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3895..3915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4313..4324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4483..4604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4494..4513)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4548..4567
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4719..4765
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4795..4931
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4976..5047
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5092..5288
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5139..5274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5302..6096
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5356..5375)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5966..5985
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6107..6331
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6607..6626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7861..7929,7930..8721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8079..8098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8734..8870
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9022..9093
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9098..9144)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9162..9210
                     /note="synthetic polyadenylation signal"
     enhancer        9291..9594
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9596..9798
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9749..9769
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9932..10407
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caatagagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggtta cttctaacca taagaataac aagtcttgga tggagtcaga gttccgcgtt
      481 tacagttccg ctaacaactg tacttttgag tacgtttccc aaccatttct catggatctt
      541 gaagggaagc aaggcaactt caagaatctc cgcgaatttg tgtttaagaa tatagacggc
      601 tacttcaaaa tttatagcaa gcataccccg ataaatctgg ttcgggatct ccctcaaggc
      661 ttctccgcgt tggaacccct cgtggacttg ccgattggca tcaacatcac ccggttccaa
      721 acccttcttg cccttcatcg aagctacctc acgcctggcg acagtagtag tggttggaca
      781 gcgggcgcag cagcctacta tgtgggatat ttgcaaccac gcacctttct tctcaaatat
      841 aacgaaaacg gaacaattac cgatgccgta gactgtgccc tcgatcccct gtccgaaact
      901 aaatgcacac tcaagtcctt cacagttgag aaaggcatat accaaaccag taacttccgc
      961 gtccaaccca ccgaatccat tgttcgattc cctaacatca ccaatttgtg tcctttcggt
     1021 gaagtattta atgccactaa atttgcatca gtttatgcct ggaatcgcaa gcgaatatca
     1081 aattgtgttg cagattatag tgtcctgtat aatagcgcat cattcagcac ttttaagtgt
     1141 tatggggtca gccctacgaa attgaatgac ttgtgtttta ccaacgttta cgcagattct
     1201 ttcgtcatac gcggcgacga agtccgacag attgcaccgg ggcaaaccgg aaagatagca
     1261 gactataatt ataagttgcc ggatgacttc accggctgcg ttatagcatg gaactccaat
     1321 aatctcgatt caaaagtcgg cggcaactat aattatcttt accgcctgtt tcgcaagtca
     1381 aatctgaagc cctttgaacg tgacatctcc accgaaattt atcaagctgg cagcacccca
     1441 tgtaatggag tgaaagggtt taactgttat tttccccttc aatcatacgg atttcaacca
     1501 acatatgggg tgggctatca accatatcgg gtggtagtct tgagctttga gctgctccac
     1561 gcaccggcta cagtttgcgg accgaagaaa agcactaacc tggtgaagaa taaatgtgtg
     1621 aacttcaact ttaatgggct caccggaaca ggggtgctga cagaatcaaa caagaaattt
     1681 ctcccttttc aacaatttgg tagagacatt gccgatacca cagatgccgt ccgcgatccg
     1741 caaacactgg aaatcctcga tataacaccc tgtagcttcg gcggggtcag cgtgataact
     1801 cccggaacga acacatccaa ccaagtggct gtcctgtatc aaggtgtcaa ctgtaccgaa
     1861 gtccctgtgg cgatacacgc cgaccaactg acacccacct ggagagtcta ctcaacaggt
     1921 tccaatgtct ttcaaactag agcgggttgt ctcattggcg ctgagcatgt caataattca
     1981 tacgaatgtg atattcctat tggggcagga atctgtgcgt cttatcaaac tcaaacgaac
     2041 tcacaccggc gcgctcgctc agtagccagc cagtccataa tcgcctatac catgagtctc
     2101 ggggctgaga actccgtggc ttattcaaac aattccatcg caatcccaac taactttacc
     2161 attagcgtta ccacggaaat cttgcccgtc agcatgacta agacgagcgt agactgtacg
     2221 atgtatatct gtggggattc caccgagtgt agcaacctct tgcttcaata cgggtctttc
     2281 tgtactcaac tcaaccgagc attgaccgga attgcggtcg aacaagacaa gaatacccaa
     2341 gaggtgttcg cacaagtaaa gcaaatatac aaaacgccgc caatcaaaga ttttggcggg
     2401 tttaatttta gccaaatact gccagaccca tctaagcctt caaaacgcag ctttatcgag
     2461 gatctgctgt tcaacaaagt tacgttggct gacgcgggtt tcattaagca gtatggggat
     2521 tgtttgggag acattgcggc ccgcgacctt atctgtgctc aaaaattcaa tgggttgaca
     2581 gttctgcccc ctctgctcac tgatgagatg attgcacagt atacatcagc attgttggca
     2641 ggaaccatta catccggctg gacttttggc gcgggtgcgg cccttcaaat acctttcgct
     2701 atgcaaatgg cttacagatt caatgggatt ggtgtcacgc aaaatgtatt gtatgaaaat
     2761 cagaagctga tcgctaatca atttaacagc gcgattggca agatccaaga ttcactgtca
     2821 agcacagcgt ccgccctcgg caaactccaa aatgttgtga atcaaaacgc ccaggccctg
     2881 aataccctcg tgaagcaact gagttctaac ttcggcgcaa tctccagcgt cctcaacgac
     2941 atattgtcca gacttgataa ggtggaagcc gaggtgcaaa ttgatcggct catcacaggc
     3001 aggctccagt cactccaaac ttatgtgaca caacagttga tccgggctgc tgagatacga
     3061 gcaagcgcca acctggccgc cactaaaatg tcagagtgcg tgctcgggca gtccaaacgg
     3121 gttgactttt gtggcaaggg ctatcacttg atgtccttcc cccaatccgc cccacatgga
     3181 gtggtctttc tccacgtcac ttatgtccct gctcaagaga agaactttac gacagctcca
     3241 gcaatctgtc acgatggcaa agcgcacttt cccagggaag gcgttttcgt cagtaatggc
     3301 acgcattggt ttgtgaccca aaggaatttc tatgaacccc aaatcataac cactgacaat
     3361 acatttgtct caggcaactg tgatgtggtc attgggatcg tgaacaacac ggtatatgat
     3421 cccctgcaac ccgagttgga tagcttcaaa gaggaattgg ataaatactt taagaatcat
     3481 acttcacccg atgttgatct cggggacata tcaggaataa atgcaagtgt cgtcaacatt
     3541 cagaaagaga ttgatcgcct taatgaggtc gccaagaact tgaatgaatc tttgatcgac
     3601 ttgcaagaac tgggcaaata cgaacaatat attaaatggc cctggtacat ttggttgggc
     3661 tttatcgccg gacttatagc tatcgttatg gtcacaatta tgttgtgctg tatgactagc
     3721 tgttgttcct gcttgaaagg atgttgcagt tgcggatctt gttgtaaata aaggatcctc
     3781 tagaagttgt ctcctcctgc actgactgac tgatacaatc gatttctgga tccgcaggcc
     3841 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3901 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3961 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4021 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4081 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4141 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4201 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     4261 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4321 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4381 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtctagct
     4441 tgactgactg agatacagcg taccttcagc tcacagacat gataagatac attgatgagt
     4501 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     4561 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     4621 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     4681 tctacaaatg tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc
     4741 taaacgggtc ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg
     4801 gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt
     4861 cagcaacgga tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa
     4921 tttatcctta aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc
     4981 ctttaatggt cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc
     5041 ctccctaatc tcattggtta ccttgggcta tcgaaactta attaagcgat ctgcatctca
     5101 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5161 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
     5221 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5281 tttgcaaagg aggtagccaa catgattgaa caagatggat tgcacgcagg ttctcccgcc
     5341 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5401 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5461 tccggtgccc tgaatgaact ccaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5521 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5581 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5641 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5701 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5761 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5821 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg
     5881 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5941 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6001 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6061 atcgccttct atcgccttct tgacgagttc ttctagtatg taagccctgt gccttctagt
     6121 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6181 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6241 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6301 aggcatgctg gggatgcggt gggctctatg gttaattaac cagtcaagtc agctacttgg
     6361 cgagatcgac ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg
     6421 gtccttgcta ttgcacccgt tctccgatta cgagtttcat ttaaatcatg tgagcaaaag
     6481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7321 cgatctgtct atttcgttca tccatagttg catttaaatt tccgaactct ccaaggccct
     7381 cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc aggctacctc tcgaacgaac
     7441 tatcgcaagt ctcttggccg gccttgcgcc ttggctattg cttggcagcg cctatcgcca
     7501 ggtattactc caatcccgaa tatccgagat cgggatcacc cgagagaagt tcaacctaca
     7561 tcctcaatcc cgatctatcc gagatccgag gaatatcgaa atcggggcgc gcctggtgta
     7621 ccgagaacga tcctctcagt gcgagtctcg acgatccata tcgttgcttg gcagtcagcc
     7681 agtcggaatc cagcttggga cccaggaagt ccaatcgtca gatattgtac tcaagcctgg
     7741 tcacggcagc gtaccgatct gtttaaacct agatattgat agtctgatcg gtcaacgtat
     7801 aatcgagtcc tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc
     7861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcg
     7981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8041 gaagaacgct ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8161 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
     8281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8401 cctgtagcaa tggcaacaac cttgcgtaaa ctattaactg gcgaactact tactctagct
     8461 tcccggcaac agttgataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8701 tcactgatta agcattggta accgattcta ggtgcattgg cgcagaaaaa aatgcctgat
     8761 gcgacgctgc gcgtcttata ctcccacata tgccagattc agcaacggat acggcttccc
     8821 caacttgccc acttccatac gtgtcctcct taccagaaat ttatccttaa gatcccgaat
     8881 cgtttaaact cgactctggc tctatcgaat ctccgtcgtt tcgagcttac gcgaacagcc
     8941 gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc tatcgtcagc ttaccttttt
     9001 ggcagcgatc gcggctcccg acatcttgga ccattagctc cacaggtatc ttcttccctc
     9061 tagtggtcat aacagcagct tcagctacct ctcaattcaa aaaacccctc aagacccgtt
     9121 tagaggcccc aaggggttat gctatcaatc gttgcgttac acacacaaaa aaccaacaca
     9181 catccatctt cgatggatag cgattttatt atctaactgc tgatcgagtg tagccagatc
     9241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     9301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     9361 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     9421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     9481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     9541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgctgatg
     9601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     9661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     9721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     9781 gtctatataa gcagagctgg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     9841 tgttttgacc tccatagaag acaccgggac cgatccagcc tcccctcgaa gcttacatgt
     9901 ggtaccgagc tcggatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct
     9961 ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca
    10021 catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt
    10081 aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca
    10141 ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc
    10201 tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa attgtaactg
    10261 atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc tgcttttatt
    10321 ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg
    10381 ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc
    10441 catcactttg gcaaaggcgg ccgcc
//
