LOCUS       sequence-43279-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Dmitriev PV, Vassetzky YS
  TITLE     A set of vectors for introduction of antibiotic resistance genes by 
            in vitro Cre-mediated recombination.
  JOURNAL   BMC Res Notes. 2008 Dec 23;1:135. doi: 10.1186/1756-0500-1-135.
  PUBMED    19105832
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2008-12-23"; volume: "1:135. doi"; pages: " 
            10.1186/1756-0500-1-135"
COMMENT     Sequence Label: pINS-Blast
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      142..498
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 caattgcggc cgcggatccc catggccata tgttctcgag cccggggaat tccagataac
       61 ttcgtataat gtatgctata cgaagttatg gtaccgcggc cgcgtagagg atctgttgat
      121 cagcagttca acctgttgat agtacgtact aagctctcat gtttcacgta ctaagctctc
      181 atgtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct aacgtactaa
      241 gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc atgtttgaac
      301 aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt tttataagaa
      361 aaaaaagaat atataaggct tttaaagctt ttaaggttta acggttgtgg acaacaagcc
      421 agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga gtgacacagg
      481 aacacttaac ggctgacatg ggaattagct tcacgctgcc gcaagcactc agggcgcaag
      541 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
      601 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
      661 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
      721 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
      781 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
      841 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg t
//
