LOCUS       sequence-432790-       10471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike R214 insertion for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 InsertionR214...
FEATURES             Location/Qualifiers
     source          1..10471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3848..4436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3901..3921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4319..4330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4489..4610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4500..4519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4554..4573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4725..4771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4801..4937
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4982..5053
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5098..5294
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5145..5280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5308..6102
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5362..5381)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5972..5991
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6113..6337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6533..7121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6613..6632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7867..7935,7936..8727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8085..8104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8740..8876
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9028..9099
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9104..9150)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9168..9216
                     /note="synthetic polyadenylation signal"
     enhancer        9297..9600
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9602..9804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9755..9775
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9938..10413
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata aatctggttc gggaacccga agatctccct
      661 caaggcttct ccgcgttgga acccctcgtg gacttgccga ttggcatcaa catcacccgg
      721 ttccaaaccc ttcttgccct tcatcgaagc tacctcacgc ctggcgacag tagtagtggt
      781 tggacagcgg gcgcagcagc ctactatgtg ggatatttgc aaccacgcac ctttcttctc
      841 aaatataacg aaaacggaac aattaccgat gccgtagact gtgccctcga tcccctgtcc
      901 gaaactaaat gcacactcaa gtccttcaca gttgagaaag gcatatacca aaccagtaac
      961 ttccgcgtcc aacccaccga atccattgtt cgattcccta acatcaccaa tttgtgtcct
     1021 ttcggtgaag tatttaatgc cactcgattt gcatcagttt atgcctggaa tcgcaagcga
     1081 atatcaaatt gtgttgcaga ttatagtgtc ctgtataata gcgcatcatt cagcactttt
     1141 aagtgttatg gggtcagccc tacgaaattg aatgacttgt gttttaccaa cgtttacgca
     1201 gattctttcg tcatacgcgg cgacgaagtc cgacagattg caccggggca aaccggaaag
     1261 atagcagact ataattataa gttgccggat gacttcaccg gctgcgttat agcatggaac
     1321 tccaataatc tcgattcaaa agtcggcggc aactataatt atctttaccg cctgtttcgc
     1381 aagtcaaatc tgaagccctt tgaacgtgac atctccaccg aaatttatca agctggcagc
     1441 accccatgta atggagtgga agggtttaac tgttattttc cccttcaatc atacggattt
     1501 caaccaacaa atggggtggg ctatcaacca tatcgggtgg tagtcttgag ctttgagctg
     1561 ctccacgcac cggctacagt ttgcggaccg aagaaaagca ctaacctggt gaagaataaa
     1621 tgtgtgaact tcaactttaa tgggctcacc ggaacagggg tgctgacaga atcaaacaag
     1681 aaatttctcc cttttcaaca atttggtaga gacattgccg ataccacaga tgccgtccgc
     1741 gatccgcaaa cactggaaat cctcgatata acaccctgta gcttcggcgg ggtcagcgtg
     1801 ataactcccg gaacgaacac atccaaccaa gtggctgtcc tgtatcaaga tgtcaactgt
     1861 accgaagtcc ctgtggcgat acacgccgac caactgacac ccacctggag agtctactca
     1921 acaggttcca atgtctttca aactagagcg ggttgtctca ttggcgctga gcatgtcaat
     1981 aattcatacg aatgtgatat tcctattggg gcaggaatct gtgcgtctta tcaaactcaa
     2041 acgaactcac cacggcgcgc tcgctcagta gccagccagt ccataatcgc ctataccatg
     2101 agtctcgggg ctgagaactc cgtggcttat tcaaacaatt ccatcgcaat cccaactaac
     2161 tttaccatta gcgttaccac ggaaatcttg cccgtcagca tgactaagac gagcgtagac
     2221 tgtacgatgt atatctgtgg ggattccacc gagtgtagca acctcttgct tcaatacggg
     2281 tctttctgta ctcaactcaa ccgagcattg accggaattg cggtcgaaca agacaagaat
     2341 acccaagagg tgttcgcaca agtaaagcaa atatacaaaa cgccgccaat caaagatttt
     2401 ggcgggttta attttagcca aatactgcca gacccatcta agccttcaaa acgcagcttt
     2461 atcgaggatc tgctgttcaa caaagttacg ttggctgacg cgggtttcat taagcagtat
     2521 ggggattgtt tgggagacat tgcggcccgc gaccttatct gtgctcaaaa attcaatggg
     2581 ttgacagttc tgccccctct gctcactgat gagatgattg cacagtatac atcagcattg
     2641 ttggcaggaa ccattacatc cggctggact tttggcgcgg gtgcggccct tcaaatacct
     2701 ttcgctatgc aaatggctta cagattcaat gggattggtg tcacgcaaaa tgtattgtat
     2761 gaaaatcaga agctgatcgc taatcaattt aacagcgcga ttggcaagat ccaagattca
     2821 ctgtcaagca cagcgtccgc cctcggcaaa ctccaagatg ttgtgaatca aaacgcccag
     2881 gccctgaata ccctcgtgaa gcaactgagt tctaacttcg gcgcaatctc cagcgtcctc
     2941 aacgacatat tgtccagact tgataaggtg gaagccgagg tgcaaattga tcggctcatc
     3001 acaggcaggc tccagtcact ccaaacttat gtgacacaac agttgatccg ggctgctgag
     3061 atacgagcaa gcgccaacct ggccgccact aaaatgtcag agtgcgtgct cgggcagtcc
     3121 aaacgggttg acttttgtgg caagggctat cacttgatgt ccttccccca atccgcccca
     3181 catggagtgg tctttctcca cgtcacttat gtccctgctc aagagaagaa ctttacgaca
     3241 gctccagcaa tctgtcacga tggcaaagcg cactttccca gggaaggcgt tttcgtcagt
     3301 aatggcacgc attggtttgt gacccaaagg aatttctatg aaccccaaat cataaccact
     3361 gacaatacat ttgtctcagg caactgtgat gtggtcattg ggatcgtgaa caacacggta
     3421 tatgatcccc tgcaacccga gttggatagc ttcaaagagg aattggataa atactttaag
     3481 aatcatactt cacccgatgt tgatctcggg gacatatcag gaataaatgc aagtgtcgtc
     3541 aacattcaga aagagattga tcgccttaat gaggtcgcca agaacttgaa tgaatctttg
     3601 atcgacttgc aagaactggg caaatacgaa caatatatta aatggccctg gtacatttgg
     3661 ttgggcttta tcgccggact tatagctatc gttatggtca caattatgtt gtgctgtatg
     3721 actagctgtt gttcctgctt gaaaggatgt tgcagttgcg gatcttgttg taaataaagg
     3781 atcctctaga agttgtctcc tcctgcactg actgactgat acaatcgatt tctggatccg
     3841 caggcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3901 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3961 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4021 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4081 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4141 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4201 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     4261 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4321 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4381 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt
     4441 ctagcttgac tgactgagat acagcgtacc ttcagctcac agacatgata agatacattg
     4501 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4561 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4621 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4681 aaaacctcta caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg
     4741 ggcctctaaa cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg
     4801 gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc
     4861 cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac
     4921 cagaaattta tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag
     4981 acattccttt aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt
     5041 tcttccctcc ctaatctcat tggttacctt gggctatcga aacttaatta agcgatctgc
     5101 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5161 cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg
     5221 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5281 taggcttttg caaaggaggt agccaacatg attgaacaag atggattgca cgcaggttct
     5341 cccgccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5401 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5461 gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc
     5521 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5581 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5641 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5701 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5761 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5821 gccaggctca aggcgcggat gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc
     5881 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5941 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6001 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6061 cagcgcatcg ccttctatcg ccttcttgac gagttcttct agtatgtaag ccctgtgcct
     6121 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6181 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6241 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6301 aatagcaggc atgctgggga tgcggtgggc tctatggtta attaaccagt caagtcagct
     6361 acttggcgag atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc
     6421 gatctggtcc ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag
     6481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7321 tctcagcgat ctgtctattt cgttcatcca tagttgcatt taaatttccg aactctccaa
     7381 ggccctcgtc ggaaaatctt caaacctttc gtccgatcca tcttgcaggc tacctctcga
     7441 acgaactatc gcaagtctct tggccggcct tgcgccttgg ctattgcttg gcagcgccta
     7501 tcgccaggta ttactccaat cccgaatatc cgagatcggg atcacccgag agaagttcaa
     7561 cctacatcct caatcccgat ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct
     7621 ggtgtaccga gaacgatcct ctcagtgcga gtctcgacga tccatatcgt tgcttggcag
     7681 tcagccagtc ggaatccagc ttgggaccca ggaagtccaa tcgtcagata ttgtactcaa
     7741 gcctggtcac ggcagcgtac cgatctgttt aaacctagat attgatagtc tgatcggtca
     7801 acgtataatc gagtcctagc ttttgcaaac atctatcaag agacaggatc agcaggaggc
     7861 tttcgcatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7981 ggtgcgcgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8041 cgccccgaag aacgctttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8101 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8161 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8281 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8401 acgatgcctg tagcaatggc aacaaccttg cgtaaactat taactggcga actacttact
     8461 ctagcttccc ggcaacagtt gatagactgg atggaggcgg ataaagttgc aggaccactt
     8521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8701 ggtgcctcac tgattaagca ttggtaaccg attctaggtg cattggcgca gaaaaaaatg
     8761 cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg
     8821 cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaagatc
     8881 ccgaatcgtt taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga
     8941 acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac
     9001 ctttttggca gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct
     9061 tccctctagt ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga
     9121 cccgtttaga ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc
     9181 aacacacatc catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc
     9241 cagatctagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     9301 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     9361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     9421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     9481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     9541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     9601 ctgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     9661 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     9721 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     9781 tgggaggtct atataagcag agctggttta gtgaaccgtc agatcgcctg gagacgccat
     9841 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
     9901 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
     9961 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
    10021 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
    10081 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
    10141 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
    10201 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
    10261 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
    10321 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
    10381 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
    10441 ctggcccatc actttggcaa aggcggccgc c
//
