LOCUS       sequence-432791-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike N211I, L212 deletion, R214 insertion for 
            pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 N211I (ATT)
            d...
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3845..4433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3898..3918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4316..4327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4486..4607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4497..4516)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4551..4570
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4722..4768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4798..4934
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4979..5050
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5095..5291
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5142..5277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5305..6099
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5359..5378)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5969..5988
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6110..6334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6530..7118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6610..6629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7864..7932,7933..8724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8082..8101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8737..8873
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9025..9096
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9101..9147)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9165..9213
                     /note="synthetic polyadenylation signal"
     enhancer        9294..9597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9599..9801
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9752..9772
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9935..10410
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata attgttcggg aacccgaaga tctccctcaa
      661 ggcttctccg cgttggaacc cctcgtggac ttgccgattg gcatcaacat cacccggttc
      721 caaacccttc ttgcccttca tcgaagctac ctcacgcctg gcgacagtag tagtggttgg
      781 acagcgggcg cagcagccta ctatgtggga tatttgcaac cacgcacctt tcttctcaaa
      841 tataacgaaa acggaacaat taccgatgcc gtagactgtg ccctcgatcc cctgtccgaa
      901 actaaatgca cactcaagtc cttcacagtt gagaaaggca tataccaaac cagtaacttc
      961 cgcgtccaac ccaccgaatc cattgttcga ttccctaaca tcaccaattt gtgtcctttc
     1021 ggtgaagtat ttaatgccac tcgatttgca tcagtttatg cctggaatcg caagcgaata
     1081 tcaaattgtg ttgcagatta tagtgtcctg tataatagcg catcattcag cacttttaag
     1141 tgttatgggg tcagccctac gaaattgaat gacttgtgtt ttaccaacgt ttacgcagat
     1201 tctttcgtca tacgcggcga cgaagtccga cagattgcac cggggcaaac cggaaagata
     1261 gcagactata attataagtt gccggatgac ttcaccggct gcgttatagc atggaactcc
     1321 aataatctcg attcaaaagt cggcggcaac tataattatc tttaccgcct gtttcgcaag
     1381 tcaaatctga agccctttga acgtgacatc tccaccgaaa tttatcaagc tggcagcacc
     1441 ccatgtaatg gagtggaagg gtttaactgt tattttcccc ttcaatcata cggatttcaa
     1501 ccaacaaatg gggtgggcta tcaaccatat cgggtggtag tcttgagctt tgagctgctc
     1561 cacgcaccgg ctacagtttg cggaccgaag aaaagcacta acctggtgaa gaataaatgt
     1621 gtgaacttca actttaatgg gctcaccgga acaggggtgc tgacagaatc aaacaagaaa
     1681 tttctccctt ttcaacaatt tggtagagac attgccgata ccacagatgc cgtccgcgat
     1741 ccgcaaacac tggaaatcct cgatataaca ccctgtagct tcggcggggt cagcgtgata
     1801 actcccggaa cgaacacatc caaccaagtg gctgtcctgt atcaagatgt caactgtacc
     1861 gaagtccctg tggcgataca cgccgaccaa ctgacaccca cctggagagt ctactcaaca
     1921 ggttccaatg tctttcaaac tagagcgggt tgtctcattg gcgctgagca tgtcaataat
     1981 tcatacgaat gtgatattcc tattggggca ggaatctgtg cgtcttatca aactcaaacg
     2041 aactcaccac ggcgcgctcg ctcagtagcc agccagtcca taatcgccta taccatgagt
     2101 ctcggggctg agaactccgt ggcttattca aacaattcca tcgcaatccc aactaacttt
     2161 accattagcg ttaccacgga aatcttgccc gtcagcatga ctaagacgag cgtagactgt
     2221 acgatgtata tctgtgggga ttccaccgag tgtagcaacc tcttgcttca atacgggtct
     2281 ttctgtactc aactcaaccg agcattgacc ggaattgcgg tcgaacaaga caagaatacc
     2341 caagaggtgt tcgcacaagt aaagcaaata tacaaaacgc cgccaatcaa agattttggc
     2401 gggtttaatt ttagccaaat actgccagac ccatctaagc cttcaaaacg cagctttatc
     2461 gaggatctgc tgttcaacaa agttacgttg gctgacgcgg gtttcattaa gcagtatggg
     2521 gattgtttgg gagacattgc ggcccgcgac cttatctgtg ctcaaaaatt caatgggttg
     2581 acagttctgc cccctctgct cactgatgag atgattgcac agtatacatc agcattgttg
     2641 gcaggaacca ttacatccgg ctggactttt ggcgcgggtg cggcccttca aatacctttc
     2701 gctatgcaaa tggcttacag attcaatggg attggtgtca cgcaaaatgt attgtatgaa
     2761 aatcagaagc tgatcgctaa tcaatttaac agcgcgattg gcaagatcca agattcactg
     2821 tcaagcacag cgtccgccct cggcaaactc caagatgttg tgaatcaaaa cgcccaggcc
     2881 ctgaataccc tcgtgaagca actgagttct aacttcggcg caatctccag cgtcctcaac
     2941 gacatattgt ccagacttga taaggtggaa gccgaggtgc aaattgatcg gctcatcaca
     3001 ggcaggctcc agtcactcca aacttatgtg acacaacagt tgatccgggc tgctgagata
     3061 cgagcaagcg ccaacctggc cgccactaaa atgtcagagt gcgtgctcgg gcagtccaaa
     3121 cgggttgact tttgtggcaa gggctatcac ttgatgtcct tcccccaatc cgccccacat
     3181 ggagtggtct ttctccacgt cacttatgtc cctgctcaag agaagaactt tacgacagct
     3241 ccagcaatct gtcacgatgg caaagcgcac tttcccaggg aaggcgtttt cgtcagtaat
     3301 ggcacgcatt ggtttgtgac ccaaaggaat ttctatgaac cccaaatcat aaccactgac
     3361 aatacatttg tctcaggcaa ctgtgatgtg gtcattggga tcgtgaacaa cacggtatat
     3421 gatcccctgc aacccgagtt ggatagcttc aaagaggaat tggataaata ctttaagaat
     3481 catacttcac ccgatgttga tctcggggac atatcaggaa taaatgcaag tgtcgtcaac
     3541 attcagaaag agattgatcg ccttaatgag gtcgccaaga acttgaatga atctttgatc
     3601 gacttgcaag aactgggcaa atacgaacaa tatattaaat ggccctggta catttggttg
     3661 ggctttatcg ccggacttat agctatcgtt atggtcacaa ttatgttgtg ctgtatgact
     3721 agctgttgtt cctgcttgaa aggatgttgc agttgcggat cttgttgtaa ataaaggatc
     3781 ctctagaagt tgtctcctcc tgcactgact gactgataca atcgatttct ggatccgcag
     3841 gcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4261 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtcta
     4441 gcttgactga ctgagataca gcgtaccttc agctcacaga catgataaga tacattgatg
     4501 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4561 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4621 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4681 acctctacaa atgtggtatt ggcccatctc tatcggtatc gtagcataac cccttggggc
     4741 ctctaaacgg gtcttgaggg gttttttgtg cccctcgggc cggattgcta tctaccggca
     4801 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag
     4861 attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag
     4921 aaatttatcc ttaaggtcgt cagctatcct gcaggcgatc tctcgatttc gatcaagaca
     4981 ttcctttaat ggtcttttct ggacaccact aggggtcaga agtagttcat caaactttct
     5041 tccctcccta atctcattgg ttaccttggg ctatcgaaac ttaattaagc gatctgcatc
     5101 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5161 ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat gcagaggccg
     5221 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5281 gcttttgcaa aggaggtagc caacatgatt gaacaagatg gattgcacgc aggttctccc
     5341 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5401 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5461 ctgtccggtg ccctgaatga actccaggac gaggcagcgc ggctatcgtg gctggccacg
     5521 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5581 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5641 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5701 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5761 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5821 aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc
     5881 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5941 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6001 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6061 cgcatcgcct tctatcgcct tcttgacgag ttcttctagt atgtaagccc tgtgccttct
     6121 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6181 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6241 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6301 agcaggcatg ctggggatgc ggtgggctct atggttaatt aaccagtcaa gtcagctact
     6361 tggcgagatc gacttgtctg ggtttcgact acgctcagaa ttgcgtcagt caagttcgat
     6421 ctggtccttg ctattgcacc cgttctccga ttacgagttt catttaaatc atgtgagcaa
     6481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6961 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7141 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7201 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7261 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7321 cagcgatctg tctatttcgt tcatccatag ttgcatttaa atttccgaac tctccaaggc
     7381 cctcgtcgga aaatcttcaa acctttcgtc cgatccatct tgcaggctac ctctcgaacg
     7441 aactatcgca agtctcttgg ccggccttgc gccttggcta ttgcttggca gcgcctatcg
     7501 ccaggtatta ctccaatccc gaatatccga gatcgggatc acccgagaga agttcaacct
     7561 acatcctcaa tcccgatcta tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt
     7621 gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca
     7681 gccagtcgga atccagcttg ggacccagga agtccaatcg tcagatattg tactcaagcc
     7741 tggtcacggc agcgtaccga tctgtttaaa cctagatatt gatagtctga tcggtcaacg
     7801 tataatcgag tcctagcttt tgcaaacatc tatcaagaga caggatcagc aggaggcttt
     7861 cgcatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7921 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7981 gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8041 cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8101 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8161 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8221 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8281 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8341 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8401 atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact acttactcta
     8461 gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg accacttctg
     8521 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8581 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8641 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8701 gcctcactga ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct
     8761 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt
     8821 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagatcccg
     8881 aatcgtttaa actcgactct ggctctatcg aatctccgtc gtttcgagct tacgcgaaca
     8941 gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc agctatcgtc agcttacctt
     9001 tttggcagcg atcgcggctc ccgacatctt ggaccattag ctccacaggt atcttcttcc
     9061 ctctagtggt cataacagca gcttcagcta cctctcaatt caaaaaaccc ctcaagaccc
     9121 gtttagaggc cccaaggggt tatgctatca atcgttgcgt tacacacaca aaaaaccaac
     9181 acacatccat cttcgatgga tagcgatttt attatctaac tgctgatcga gtgtagccag
     9241 atctagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     9301 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     9361 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     9421 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     9481 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     9541 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgctg
     9601 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     9661 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     9721 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     9781 gaggtctata taagcagagc tggtttagtg aaccgtcaga tcgcctggag acgccatcca
     9841 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc gaagcttaca
     9901 tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt
     9961 tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt
    10021 acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct
    10081 tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg
    10141 gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat
    10201 ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa
    10261 ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt
    10321 attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc
    10381 atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg
    10441 gcccatcact ttggcaaagg cggccgcc
//
